Identification and characterization of phytoplasma associated with brinjal little leaf, phyllody and witches’ broom disease in four states of India

Authors

  • MEGHA MAHESHWARI, MANISH KUMAR*, MADHUPRIYA and G.P. RAO

DOI:

https://doi.org/10.24838/ip.2017.v70.i2.70754

Keywords:

BLAST, brinjal, In silico RFLP, PCR assay, Phytoplasma

Abstract

Symptoms of little leaf, phyllody and witches’ broom on brinjal plants were recorded at New Delhi, Uttar Pradesh, Maharashtra and Chhattisgarh states of India in 2016. The disease incidence was recorded in the range 5-20% in different surveyed fields. PCR assays were performed using universal primers specific to the phytoplasma 16S rRNA gene: P1/P7 followed by 3F/3R which yielded 1.3 kb amplified products in nested PCR assays in eight symptomatic plants from all the four surveyed states but not from any healthy controls. Pairwise 16S rDNA sequence comparison and phylogenetic analysis of BLL isolates revealed their close relationship with strains of ‘Candidatus Phytoplasma trifolii' (16SrVI group). In silico RFLP analysis through pDraw32 program further confirmed subgrouping of the phytoplasma strains associated with brinjal little leaf and witches’ broom disease in 16SrVI-D subgroup. 

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Issue

Vol. 70 No. 2 (2017): Indian Phytopathology

Section

Short Communications

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How to Cite

MADHUPRIYA and G.P. RAO, M. M. M. K. (2017). Identification and characterization of phytoplasma associated with brinjal little leaf, phyllody and witches’ broom disease in four states of India. Indian Phytopathology, 70(2), 258-261. https://doi.org/10.24838/ip.2017.v70.i2.70754