Immunological effect of sparfloxacin in goats

Vivek Pratap Singh; RAMESH  KUMAR NIRALA; V K  GOND; S K  GANGWAR; K  ANJANA; C  JAYACHANDRAN; M K  SINGH; R P  SINGH; V P  SINGH

doi:10.56093/ijans.v92i5.112830

Authors

Vivek Pratap Singh Mahayogi Gorakhnath Krishi Vigyan Kendra Peppeganj, Gorakhpur, U. P. India
RAMESH KUMAR NIRALA Bihar Animal Sciences University, Patna, Bihar 800 014 India
V K GOND Bihar Animal Sciences University, Patna, Bihar 800 014 India
S K GANGWAR Bihar Animal Sciences University, Patna, Bihar 800 014 India
K ANJANA Bihar Animal Sciences University, Patna, Bihar 800 014 India
C JAYACHANDRAN Bihar Animal Sciences University, Patna, Bihar 800 014 India
M K SINGH Bihar Animal Sciences University, Patna, Bihar 800 014 India
R P SINGH Bihar Animal Sciences University, Patna, Bihar 800 014 India
V P SINGH Bihar Animal Sciences University, Patna, Bihar 800 014 India

https://doi.org/10.56093/ijans.v92i5.112830

Abstract

In the present study, the immunological effects of a fluoroquinolone, sparfloxacin, were studied in goats after
multiple i.m. administration. Three groups of goats (4 goats in each) were selected. Group I consisted of saline control, Group II contained animals with antigen (Ag) control [2 ml of 7% sheep red blood cell (SRBC), Ag given on 1st day as sensitizing dose and on 10th day as challenging dose] and Group III consisted of animals exposed to sparfloxacin and SRBC Ag (Sparfloxacin @ 5 mg/kg i.m. daily for 7 days during sensitizing and challenging period and SRBC as given in Group II). The blood samples were collected on 1, 7, 10, 14, 21, 28, 35 and 42 days of the experiment. The immunological parameters evaluated were haemagglutination (HA) test for humoural immune response (HIR) whereas delayed type hypersensitivity (DTH) for evaluating cell mediated immune response (CMIR). DTH was assessed by injecting 1-chloro-2,4-dinitrobenzene (DNCB) and purified protein derivative (PPD) in the skin of the neck region as well as by absolute lymphocyte count. There was no significant effect of sparfloxacin on humoural immune response as assessed by HA test. DTH assessed by DNCB mitogen was significantly higher in sparfloxacin treated group as compared to antigen treated whereas in case of PPD mitogen there was no significant effect of the drug. Absolute lymphocyte count differed significantly in sparfloxacin treated group (6499.75±19.87) with antigen treated group (5485.00±19.87). Thus, it can be concluded that sparfloxacin has some immunomodulatory effect with respect to cell mediated immune response in goats.In the present study, the immunological effects of a fluoroquinolone, sparfloxacin, were studied in goats after multiple i.m. administration. Three groups of goats (4 goats in each) were selected. Group I consisted of saline control, Group II contained animals with antigen (Ag) control [2 ml of 7% sheep red blood cell (SRBC), Ag given on 1st day as sensitizing dose and on 10th day as challenging dose] and Group III consisted of animals exposed to sparfloxacin and SRBC Ag (Sparfloxacin @ 5 mg/kg i.m. daily for 7 days during sensitizing and challenging period and SRBC as given in Group II). The blood samples were collected on 1, 7, 10, 14, 21, 28, 35 and 42 days of the experiment. The immunological parameters evaluated were haemagglutination (HA) test for humoural immune response (HIR) whereas delayed type hypersensitivity (DTH) for evaluating cell mediated immune response (CMIR). DTH was assessed by injecting 1-chloro-2,4-dinitrobenzene (DNCB) and purified protein derivative (PPD) in the skin of the neck region as well as by absolute lymphocyte count. There was no significant effect of sparfloxacin on humoural immune response as assessed by HA test. DTH assessed by DNCB mitogen was significantly higher in sparfloxacin treated group as compared to antigen treated whereas in case of PPD mitogen there was no significant effect of the drug. Absolute lymphocyte count differed significantly in sparfloxacin treated group (6499.75±19.87) with antigen treated group (5485.00±19.87). Thus, it can be concluded that sparfloxacin has some immunomodulatory effect with respect to cell mediated immune response in goats.

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Author Biographies

Vivek Pratap Singh, Mahayogi Gorakhnath Krishi Vigyan Kendra Peppeganj, Gorakhpur, U. P. India

SMS Animal Science
Mahayogi Gorakhnath Krishi Vigyan Kendra
Peppeganj, Gorakhpur, U. P. India
RAMESH KUMAR NIRALA, Bihar Animal Sciences University, Patna, Bihar 800 014 India

In the present study, the immunological effects of a fluoroquinolone, sparfloxacin, were studied in goats after multiple i.m. administration. Three groups of goats (4 goats in each) were selected. Group I consisted of saline control, Group II contained animals with antigen (Ag) control [2 ml of 7% sheep red blood cell (SRBC), Ag given on 1st day as sensitizing dose and on 10th day as challenging dose] and Group III consisted of animals exposed to sparfloxacin and SRBC Ag (Sparfloxacin @ 5 mg/kg i.m. daily for 7 days during sensitizing and challenging period and SRBC as given in Group II). The blood samples were collected on 1, 7, 10, 14, 21, 28, 35 and 42 days of the experiment. The immunological parameters evaluated were haemagglutination (HA) test for humoural immune response (HIR) whereas delayed type hypersensitivity (DTH) for evaluating cell mediated immune response (CMIR). DTH was assessed by injecting 1-chloro-2,4-dinitrobenzene (DNCB) and purified protein derivative (PPD) in the skin of the
neck region as well as by absolute lymphocyte count. There was no significant effect of sparfloxacin on humoural immune response as assessed by HA test. DTH assessed by DNCB mitogen was significantly higher in sparfloxacin treated group as compared to antigen treated whereas in case of PPD mitogen there was no significant effect of the drug. Absolute lymphocyte count differed significantly in sparfloxacin treated group (6499.75±19.87) with antigen treated group (5485.00±19.87). Thus, it can be concluded that sparfloxacin has some immunomodulatory effect with respect to cell mediated immune response in goats.
V K GOND, Bihar Animal Sciences University, Patna, Bihar 800 014 India

In the present study, the immunological effects of a fluoroquinolone, sparfloxacin, were studied in goats after multiple i.m. administration. Three groups of goats (4 goats in each) were selected. Group I consisted of saline control, Group II contained animals with antigen (Ag) control [2 ml of 7% sheep red blood cell (SRBC), Ag given on 1st day as sensitizing dose and on 10th day as challenging dose] and Group III consisted of animals exposed to sparfloxacin and SRBC Ag (Sparfloxacin @ 5 mg/kg i.m. daily for 7 days during sensitizing and challenging period and SRBC as given in Group II). The blood samples were collected on 1, 7, 10, 14, 21, 28, 35 and 42 days of the experiment. The immunological parameters evaluated were haemagglutination (HA) test for humoural immune response (HIR) whereas delayed type hypersensitivity (DTH) for evaluating cell mediated immune response (CMIR). DTH was assessed by injecting 1-chloro-2,4-dinitrobenzene (DNCB) and purified protein derivative (PPD) in the skin of the
neck region as well as by absolute lymphocyte count. There was no significant effect of sparfloxacin on humoural immune response as assessed by HA test. DTH assessed by DNCB mitogen was significantly higher in sparfloxacin treated group as compared to antigen treated whereas in case of PPD mitogen there was no significant effect of the drug. Absolute lymphocyte count differed significantly in sparfloxacin treated group (6499.75±19.87) with antigen treated group (5485.00±19.87). Thus, it can be concluded that sparfloxacin has some immunomodulatory effect with respect to cell mediated immune response in goats

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