Statistical evaluation of modalities for diagnosis of Anaplasmosis in dairy cattle

RAVINA; Y  SINGH; J  KAUR; R  JHAMBH; G  CHARAYA

doi:10.56093/ijans.v95i8.164401

Authors

RAVINA Department of Veterinary Medicine, College of Veterinary Sciences, LUVAS, Hisar, Haryana, India
Y SINGH Department of Veterinary Medicine, College of Veterinary Sciences, LUVAS, Hisar, Haryana, India
J KAUR Department of Veterinary Medicine, College of Veterinary Sciences, LUVAS, Hisar, Haryana, India
R JHAMBH Department of Veterinary Medicine, College of Veterinary Sciences, LUVAS, Hisar, Haryana, India
G CHARAYA Department of Veterinary Medicine, College of Veterinary Sciences, LUVAS, Hisar, Haryana, India

https://doi.org/10.56093/ijans.v95i8.164401

Keywords:

Anaplasmosis, cELISA, Microscopy, msp4 protein, msp5 gene, PCR assay

Abstract

Anaplasmosis is an important tick borne rickettsial disease affecting bovines. Disease diagnosis plays an important role in limiting the spread of infection because of persistence of its carrier state. Conventional microscopy of stained blood smear, serological assay and molecular tests are commonly used for the diagnosis of anaplasmosis. The present study was planned to compare different diagnostic methods for detection of Anaplasma spp. in blood samples of cattle. A total of 72 blood and serum samples were collected from cattle suspected to be suffering from anaplasmosis. Paired stained blood smear was examined for characteristic dot shaped intraerythrocytic form of organism. Serum samples were processed for competitive ELISA for the presence of antibodies to Anaplasma spp. DNA was extracted and subjected to PCR assay for detection of Anaplasma marginale targeting msp4 gene. Out of 72 samples, 91.66% of cases were found to be positive by cELISA, followed by 56.94% by PCR assay and only 25% by microscopy. Microscopy was found to be 100% specific whereas ELISA was found to be 100% sensitive with microscopy showing 100% positive predictive value and ELISA showing 100% negative predictive value. For early and rapid detection, PCR assay should be employed, while for rapid screening of herd and control strategies cELISA may be used but in routine practice, microscopic examination can suffice.

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