Immune response study of outer membrane vesicles from Salmonella enterica serovar Typhimurium var. Weltevreden (S. Weltevreden) associated with poultry enteritis

PREETI  GODARA; DEEPAK KUMAR  SHARMA; RAJESH  SINGATHIA; ABHISHEK  GAURAV; POOJA

doi:10.56093/ijans.v96i6.172893

Authors

PREETI GODARA College of Veterinary and Animal Science (CVAS) Navania, Udaipur 313 601 Rajasthan India
DEEPAK KUMAR SHARMA College of Veterinary and Animal Science (CVAS) Navania, Udaipur 313 601 Rajasthan India
RAJESH SINGATHIA College of Veterinary and Animal Science (CVAS) Navania, Udaipur 313 601 Rajasthan India
ABHISHEK GAURAV College of Veterinary and Animal Science, Navania, Udaipur, Rajasthan, India. Rajasthan University of Veterinary and Animal Sciences, Bikaner, Rajasthan India
POOJA College of Veterinary and Animal Science (CVAS) Navania, Udaipur 313 601 Rajasthan India

https://doi.org/10.56093/ijans.v96i6.172893

Keywords:

Humoral response, Poultry Enteritis, Outer membrane vesicles, Salmonella Weltevreden

Abstract

Salmonella enterica serovar Typhimurium var. Weltevreden (S. Weltevreden) is an important foodborne pathogen in South-East Asia and poses a growing public health risk. The present study evaluated the potential of an outer- membrane vesicle (OMV) based vaccine derived from poultry-associated isolates to stimulate immune protection in chickens. Young birds were immunized through different routes (intranasal (I.N.), intramuscular (I.M.), or subcutaneous (S.C.) routes), along with a non-vaccinated control. All vaccinated groups received a booster dose on (14^th day post vaccination). Serum samples were collected at regular intervals (0, 7^th, 14^th, 21^st, and 28^th day post-vaccination) to monitor IgG responses using indirect ELISA. Birds vaccinated through S.C. or I.N. route demonstrated an early (7^th DPV) and significant rise in antibody levels after the primary dose (p ≤ 0.01), followed by stabilization until booster administration. A marked enhancement in IgG response was observed after the booster vaccination, which continued to strengthen over the remaining observation period. I.M. vaccination produced a more gradual initial response but showed clear improvement following the booster. Overall, the study indicated that OMV- based vaccination particularly via subcutaneous and intranasal routes can effectively stimulate humoral immunity in chickens. These findings highlighted the promise of OMV formulations as a control strategy for Salmonella infections in poultry, with potential benefits for reducing zoonotic transmission. Further clinical and immunological validation is warranted to determine their protective value under field conditions.

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