First report on molecular characterization of Streptococcus pluranimalium from Sirohi goat

G  NAGARAJAN; GHANSHYAM  YADAV; RAJIV KUMAR; D K  SHARMA; A S  MEENA; G G  SONAWANE; S S  MISRA; ARUN  KUMAR

doi:10.56093/ijans.v96i6.174117

Authors

G NAGARAJAN ICAR-Central Sheep and Wool Research Institute, Avikanagar Rajasthan 304 501 India
GHANSHYAM YADAV ICAR-Central Sheep and Wool Research Institute, Avikanagar Rajasthan 304 501 India
RAJIV KUMAR ICAR-Central Sheep and Wool Research Institute, Avikanagar Rajasthan 304 501 India
D K SHARMA ICAR-Central Sheep and Wool Research Institute, Avikanagar Rajasthan 304 501 India
A S MEENA ICAR-Central Sheep and Wool Research Institute, Avikanagar Rajasthan 304 501 India
G G SONAWANE ICAR-Central Sheep and Wool Research Institute, Avikanagar Rajasthan 304 501 India
S S MISRA ICAR-Central Sheep and Wool Research Institute, Avikanagar Rajasthan 304 501 India
ARUN KUMAR ICAR-Central Sheep and Wool Research Institute, Avikanagar Rajasthan 304 501 India

https://doi.org/10.56093/ijans.v96i6.174117

Keywords:

16S RNA PCR, Bioinformatics analysis, Cutaneous abscess, Sequencing, Sirohi goat, Streptococcus pluranimalium

Abstract

The present study was done with an objective to identify the causative agent(s) involved in the causation of cutaneous abscessation in goat from semi-arid region of Rajasthan, India by using conventional bacteriological and PCR protocols targeting 16S ribosomal RNA gene. During January 2025, pus sample was collected from a large sized palpable cutaneous abscess of an adult female Sirohi goat and cultured on BHI agar plate. The amplicons obtained out of universal 16S ribosomal RNA using the chromosomal DNA isolated from the bacterial colonies grown on BHI agar plate, were cloned into E. coli based vector, and the confirmed recombinant plasmids were subjected for Sanger sequencing. The organism specific genes, recN and galactokinase were also targeted and sequenced after the gene cloning experiments. The obtained nucleotide sequences of both 16S ribosomal RNA and galactokinase genes of the identified bacterial species from goat were analysed using the bioinformatics tools. Upon sequence analysis, it was found that the obtained nucleotide sequences had 99 % identity with 16 S ribosomal RNA gene of Streptococcus pluranimalium. Both recN and galactokinase genes also had 100 % identity with that of S. pluranimalium. In the phylogenetic tree analysis, S. pluranimalium from Indian goat clustered with S. pluranimalium from other host species.

Downloads

Download data is not yet available.

References

Altschul S F, Gish W, Miller W, Myers E W and Lipman D J. 1990. Basic local alignment search tool. Journal of Molecular Biology 215: 403. https://doi.org/10.1016/S0022-2836(05)80360-2. DOI: https://doi.org/10.1016/S0022-2836(05)80360-2

Devriese L A, Vandamme P, Collins M D, Alvarez N, Pot B, Hommez J, Butaye P and Haesebrouck F. 1999. Streptococcus pluranimalium sp. nov., from cattle and other animals. International Journal of Systematic Bacteriology 49(3): 1221–1226 https://doi.org/10.1099/00207713-49-3-1221. DOI: https://doi.org/10.1099/00207713-49-3-1221

Duriseti P and Fleisher J. 2019. Streptococcus pluranimalium infective endocarditis and brain abscess. IDCases 18:e00587. https://doi.org/10.1016/j.idcr.2019.e00587. DOI: https://doi.org/10.1016/j.idcr.2019.e00587

Fu D J, Ramachandran A and Miller C. 2021. Streptococcus pluranimalium meningoencephalitis in a horse. Journal of Veterinary Diagnostic Investigation 33(5):956-960. https:// doi.org/10.1177/10406387211023465. DOI: https://doi.org/10.1177/10406387211023465

George K, Jacob G, John S B and Pai S. 2020. Pneumonia with synpneumonic effusion and bacteraemia: Streptococcus pluranimalium infection in a healthy adult. British Journal of Hospital Medicine 81(2): 1–3. https://doi.org/10.12968/ hmed.2019.0275. DOI: https://doi.org/10.12968/hmed.2019.0275

Hedegaard L, Christensen H, Chadfield M S, Christensen J P and Bisgaard M. 2009. Association of Streptococcus pluranimalium with valvular endocarditis and septicaemia in adult broiler parents. Avian Pathology 38(2): 155–160. https://doi.org/10.1080/03079450902737763. DOI: https://doi.org/10.1080/03079450902737763

Kitamura Y, Kusajima Y, Okamoto M, Ueno Y, Mada T, Okura M and Takamatsu D. 2023. Development of a Streptococcus pluranimalium-specific PCR assay. Journal of Microbiological Methods 211: 106766. https://doi.org/10.1016/j.mimet.2023.106766. DOI: https://doi.org/10.1016/j.mimet.2023.106766

Kumar J, Singh F, Tripathi B N, Kumar R, Dixit S K and Sonawane G G. 2012. Epidemiological, bacteriological and molecular studies on caseous lymphadenitis in Sirohi goats of Rajasthan, India. Tropical Animal Health and Production 44(7): 1319–1322. https://doi.org/10.1007/s11250-012-0102-8. DOI: https://doi.org/10.1007/s11250-012-0102-8

Kumar J, Tripathi B N, Kumar R, Sonawane G G and Dixit S K. 2013. Rapid detection of Corynebacterium pseudotuberculosis in clinical samples from sheep. Tropical Animal Health and Production 45(6): 1429–1435. https://doi.org/10.1007/ s11250-013-0381-8. DOI: https://doi.org/10.1007/s11250-013-0381-8

Madeira F, Madhusoodanan N, Lee J, Eusebi A, Niewielska A, Tivey A R N, Lopez R and Butcher S. 2024. The EMBL-EBI Job Dispatcher sequence analysis tools framework in 2024. Nucleic Acids Research 52(1): 521–525. https://doi.org/10.1093/nar/gkae241. DOI: https://doi.org/10.1093/nar/gkae241

Pan Y, An H, Fu T, Zhao S, Zhang C, Xiao G, Zhang J, Zhao X and Hu G. 2018. Characterization of Streptococcus pluranimalium from cattle with mastitis by whole genome sequencing and functional validation. BMC Microbiology 18(1): 182. https:// doi.org/10.1186/s12866-018-1327-0. DOI: https://doi.org/10.1186/s12866-018-1327-0

Sohier M S, Selim S A, Bakry M A, Elgabry E A and Atta R M. 2017. Comparison of protection against caseous lymphadenitis in sheep induced by local isolated strain of Corynebacterium pseudotuberculosis by toxoid PLD and toxoid PLD with bacterin vaccine. International Journal of Development Research 7(9): 15326–15334.

Sunder J. De A K, Sujatha T, Chakraborty G, Mayuri S C, Bhattacharya D, Alyethodi, R R and Chakurkar E B. 2024. Isolation and molecular characterization of Corynebacterium pseudotuberculosis from goats in Andaman and Nicobar Islands, India. Microbiology Research 15(4): 2274–2285. https://doi.org/10.3390/microbiolres15040152. DOI: https://doi.org/10.3390/microbiolres15040152

Tarhane S and Büyük F. 2024. The etiological and antimicrobial susceptibility profiles of the bacteria obtained from ovine caseous lymphadenitis cases in the Çankırı region, Türkiye. Life (Basel) 14(9): 1078. https://doi.org/10.3390/life14091078. DOI: https://doi.org/10.3390/life14091078

Tripathi B, Kumar J, Sonawane G, Kumar R and Dixit S. 2016. Microbiological and molecular investigation of clinically suspected caseous lymphadenitis cases in goats. Agricultural Research 5(4): 413–419. DOI: https://doi.org/10.1007/s40003-016-0233-7

Twomey D F, Carson T, Foster G, Koylass M S and Whatmore A M. 2012. Phenotypic characterisation and 16S rRNA sequence analysis of veterinary isolates of Streptococcus pluranimalium. Veterinary Journal 192(2): 236–238. https://doi.org/10.1016/j. tvjl.2011.05.007. DOI: https://doi.org/10.1016/j.tvjl.2011.05.007

Yee M, Trimble M J, Ghosh K, Hughes G, Knowles D, Duan J, Raverty S, McGregor G and Hsiao W W L. 2025. The genotypic characterization of Streptococcus pluranimalium from aborted bovine fetuses in British Columbia, Canada. Frontiers in Microbiology l16: 1603770. https://doi. org/10.3389/fmicb.2025.1603770. DOI: https://doi.org/10.3389/fmicb.2025.1603770