Phenotypic and molecular characterization of Escherichia coli isolated from food producing small ruminants and their environment in Awadh region of Uttar Pradesh

V  YADAV; R P  DIWAKAR; D P  SHRIVASTVA; S V  SINGH; D  NIYOGI; RISHIKANT

doi:10.56093/ijans.v96i6.174343

Authors

V YADAV Acharya Narendra Deva University of Agriculture and Technology, Kumarganj Ayodhya 224 229 Uttar Pradesh India
R P DIWAKAR Acharya Narendra Deva University of Agriculture and Technology, Kumarganj Ayodhya 224 229 Uttar Pradesh India
D P SHRIVASTVA Acharya Narendra Deva University of Agriculture and Technology, Kumarganj Ayodhya 224 229 Uttar Pradesh India
S V SINGH Acharya Narendra Deva University of Agriculture and Technology, Kumarganj Ayodhya 224 229 Uttar Pradesh India
D NIYOGI Acharya Narendra Deva University of Agriculture and Technology, Kumarganj Ayodhya 224 229 Uttar Pradesh India
RISHIKANT Acharya Narendra Deva University of Agriculture and Technology, Kumarganj Ayodhya 224 229 Uttar Pradesh India

https://doi.org/10.56093/ijans.v96i6.174343

Keywords:

AMR, E. coli, ESBLs, Public health, Small ruminants

Abstract

Antimicrobial resistance (AMR) and emergence of resistance to β-lactam antibiotics especially in Enterobacteriaceae is a major global concern. The present study, total 200 samples were collected from Ayodhya and Barabanki districts of Awadh region of U.P. Out of 200 samples 46.5% were confirmed as E. coli isolates by PCR analysis using species specific uspA gene with highest 60.0% from goat faecal samples followed by 56.0%, 40.0%, 36.0% and 28.0% from sheep faecal, environmental floor swab, chevon and water samples, respectively. About 74.19% and 72.04% isolates were confirmed as ESBL producers by DDST and ESBL E-strip tests, respectively. Genotypic analysis revealed the overall prevalence of bla-CTX-M-1 (72.91%) followed by bla-TEM and bla-SHV with 43.75% and 29.17%, respectively. Antibiogram of all phenotypically confirmed E. coli isolates was done against 20 antibiotics of 12 different classes, which revealed 76.47% to 100% resistance against ampicillin, and 3^rd and 4^th generation cephalosporins, while 100% sensitive to aminoglycosides, polymyxins B and chloramphenicol. Findings of this study suggested routine AMR monitoring of animal origin food, regulated use of antibiotics in food animals, awareness among stockholders and improved hygiene practices even small herds of sheep and goat and meat shops. Thus, a comprehensive one health strategy is need of hour to control AMR and secure food safety and public health.

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