Molecular characterization of Omp31 gene of Indian field Isolates of Brucella melitensis


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Authors

  • S RAJAGUNALAN
  • GITA KUMARI
  • S K GUPTA
  • ASHOK KUMAR
  • R K AGARWAL
  • D B RAWOOL
  • D K SINGH

Keywords:

Brucella melitensis, Omp31 gene sequence

Abstract

PCR amplification and sequencing of Omp31 gene of 19 Indian field isolates of Brucella melitensis was performed. The sequence analysis revealed that all 19 isolated had 100% nucleotide sequence identity confirming the fact that the Omp31 gene of B. melitensis is highly conserved and stable. The amino acid sequences also revealed 100% sequence homology with published sequences. The protein structure predicted using the amino acid sequences confirms that this porin protein consists of 8 Beta barrels and 3 surface exposed loop portions.

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Author Biographies

  • S RAJAGUNALAN
    Ph.D. Scholar, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 India

  • GITA KUMARI
    Veterinary Officer, Department of Animal Husbandry, Government of Bihar.


  • S K GUPTA
    Scientist, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 India



  • ASHOK KUMAR
    Head, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 India

  • R K AGARWAL
    Head, Division of Bacteriology and Mycology, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 India

  • D B RAWOOL
    Senior Scientist, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 India

  • D K SINGH
    Principal Scientist, Division of Veterinary Public Health; Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 India



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Issue

Vol. 83 No. 7 (2013)

Section

Articles

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The copyright of the articles published in The Indian Journal of Animal Sciences is vested with the Indian Council of Agricultural Research, which reserves the right to enter into any agreement with any organization in India or abroad, for reprography, photocopying, storage and dissemination of information. The Council has no objection to using the material, provided the information is not being utilized for commercial purposes and wherever the information is being used, proper credit is given to ICAR.

How to Cite

RAJAGUNALAN, S., KUMARI, G., GUPTA, S. K., KUMAR, A., AGARWAL, R. K., RAWOOL, D. B., & SINGH, D. K. (2013). Molecular characterization of Omp31 gene of Indian field Isolates of Brucella melitensis. The Indian Journal of Animal Sciences, 83(7). http://epubs.icar.org.in/index.php/IJAnS/article/view/31312