Microplate-based incubation method for quality assessment of eggs of Indian major carp Catla catla

Jyotirmaya Mohanty; Sonalina Sahoo; Mohan R. Badhe; Ipsita Iswari Das; Priyanka Das; Avinash R. Rasal; Khuntia Murmu; Kanta Das Mahapatra; Pramoda Kumar Sahoo; Jitendra Kumar Sundaray

doi:10.21077/ijf.2023.70.3.129755-19

Authors

Jyotirmaya Mohanty
Sonalina Sahoo ICAR-Central Institute of Freshwater Aquaculture
Mohan R. Badhe ICAR-Central Institute of Freshwater Aquaculture
Ipsita Iswari Das ICAR-Central Institute of Freshwater Aquaculture
Priyanka Das ICAR-Central Institute of Freshwater Aquaculture
Avinash R. Rasal ICAR-Central Institute of Freshwater Aquaculture
Khuntia Murmu ICAR-Central Institute of Freshwater Aquaculture
Kanta Das Mahapatra ICAR-Central Institute of Freshwater Aquaculture
Pramoda Kumar Sahoo ICAR-Central Institute of Freshwater Aquaculture
Jitendra Kumar Sundaray ICAR-Central Institute of Freshwater Aquaculture

https://doi.org/10.21077/ijf.2023.70.3.129755-19

Keywords:

Catla catla, Microplate, Egg quality, Hatching rate

Abstract

Incubation of fertilised eggs from the Indian major carp species, Catla catla was carried
out in tissue culture microplates to assess the quality in terms of hatching rate and posthatch
survival rate. Eggs from five females (F1, F2, F3, F5 and F6) were strip spawned and
fertilised with milt from a single male. The fertilised eggs were distributed at one egg per
well of 24 well microplates, with three plates for each female. The plates were incubated at
30oC in an incubator with continuous shaking and complete hatching was observed by 36 h.
The hatching rate varied within a range of 0 to 52.8% showing the maximum of 5% for F5
followed by F1 (50%) which were significantly higher than the other three females (F2-18.1,
F6-5.6 and F3-0%). The eggs from these two females (F1 and F5) thus could be considered
as good. The survival percentage of hatchlings recorded for a period of 8 days post-hatching
was found to vary from 58.3 to 83.3 and there was low variability among different females on
any observed day. Between these two egg quality determinants, the hatching rate was found
to be more suitable considering the wide variability shown by different females indicating
the quality difference. The microplate method seems to be an appropriate tool to assess the
egg quality in catla and can be extended to other species as well.

Keywords:
Catla catla, Egg quality, Hatching rate, Microplate

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Author Biography

Jyotirmaya Mohanty

Principal Scientist, Fish Genetics and Biotechnology Division

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