Substituting dietary fishmeal with silkworm pupae meal in diets of Pacific white shrimp (Penaeus vannamei): Effects on growth performance, nutrient utilisation, whole-body composition and digestive enzyme activities

Govindharaj Sathishkumar; Nathan Felix; Amit Ranjan; Mir Ishfaq Nazir; Elangovan Prabu; Kalidoss Manikandan

doi:10.21077/ijf.2023.70.3.133916-11

Authors

Govindharaj Sathishkumar Tamil Nadu Dr. J. Jayalalithaa Fisheries University (TNJFU), Institute of Fisheries Post Graduate Studies (IFPGS), OMR – Campus, Chennai - 603 103, Tamil Nadu, India. https://orcid.org/0000-0001-5498-9027
Nathan Felix Tamil Nadu Fisheries University https://orcid.org/0000-0003-3530-5923
Amit Ranjan Tamil Nadu Dr. J. Jayalalithaa Fisheries University (TNJFU), Institute of Fisheries Post Graduate Studies (IFPGS), OMR – Campus, Chennai - 603 103, Tamil Nadu, India. https://orcid.org/0000-0002-8686-1164
Mir Ishfaq Nazir Tamil Nadu Dr. J. Jayalalithaa Fisheries University (TNJFU), Directorate of Incubation and Vocational Training in Aquaculture (DIVA), ECR – Muttukadu, Chennai – 603 112, Tamil Nadu, India. https://orcid.org/0000-0002-4039-7562
Elangovan Prabu Tamil Nadu Dr. J. Jayalalithaa Fisheries University (TNJFU), Directorate of Incubation and Vocational Training in Aquaculture (DIVA), ECR – Muttukadu, Chennai – 603 112, Tamil Nadu, India. https://orcid.org/0000-0002-1612-1638
Kalidoss Manikandan Tamil Nadu Dr. J. Jayalalithaa Fisheries University (TNJFU), Directorate of Incubation and Vocational Training in Aquaculture (DIVA), ECR – Muttukadu, Chennai – 603 112, Tamil Nadu, India. https://orcid.org/0000-0002-0072-6704

https://doi.org/10.21077/ijf.2023.70.3.133916-11

Keywords:

Amylase, Feed ingredient, Waste utilization, Weight gain

Abstract

The present study evaluated the dietary substitution effect of silkworm pupae meal (SWP) for
fish meal (FM) on growth performance, feed utilisation, whole-body proximate composition,
amino acid profile and digestive enzyme activities of Pacific white shrimp (Penaeus
vannamei). The shrimp were fed with six isonitrogenous (36% crude protein), isolipidic (8%
crude lipid) and isocaloric (16 MJ kg-1) experimental diets, containing 0, 20, 40, 60, 80 and
100% SWP, respectively, to replace dietary FM. A total of 360 shrimps (average initial weight
of 3.86±0.20 g) were distributed into 18 tanks (20 shrimp per tank). The experimental diets
were fed to triplicate groups of P. vannamei three times a day until satiation for 45 days.
Significantly (p<0.05) higher growth performance and feed utilisation were found in shrimp
fed diet with 60% incorporation level of SWP meal. No significant differences (p>0.05) were
observed in the survival rate, whole-body proximate composition and amino acid profile of
shrimp fed SWP meal-incorporated diets. Additionally, higher specific activities of digestive
enzymes such as amylase, trypsin and lipase were observed in shrimps fed diet with 60%
incorporation level of SWP meal. The present study revealed that dietary fish meal protein
up to 60% can be substituted with SWP without compromising growth performance, feed
utilisation, whole-body proximate composition, amino acid profile and digestive enzyme
activities of Pacific white shrimp fed on SWP meal based diets.

Keywords:
Amylase, Feed ingredient, Waste utilisation,
Weight gain

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