Pathogenicity of Aeromonas spp. in carp polyculture systems in the central valley zone of Assam, North-east India: Prevalence of Motile Aeromonas Septicemia in the carp polyculture system

Pabitra Kumar Saharia; Inam Akhtar Hussain; Arup Kumar Sarma; Kaustubh Bhagabati; Sangipran Baishya; Naznin Ahmed; Hemanta Pokhrel; Joynal Abedin; Jyotismita Thakuria; Dipanka Nath

doi:10.21077/ijf.2024.71.4.143618-13

Authors

Pabitra Kumar Saharia Associate Professor
Inam Akhtar Hussain Assistant Professor
Arup Kumar Sarma Professor
Kaustubh Bhagabati Assistant Professor
Sangipran Baishya Assistant Professor
Naznin Ahmed Assistant Professor
Hemanta Pokhrel Assistant Professor
Joynal Abedin
Jyotismita Thakuria
Dipanka Nath PhD Scholar

https://doi.org/10.21077/ijf.2024.71.4.143618-13

Keywords:

Motile Aeromonas, pathogen, pathogenicity, septicaemia

Abstract

The present study was undertaken to find the incidence of motile aeromonas septicemia (MAS) in the freshwater aquaculture system in three districts viz. Nagaon, Morigaon and Sonitpur of Assam. A total of 293 ponds of varying size were surveyed during the disease outbreak and thirteen different diseases were recorded. For the first time, the study of severity of MAS in freshwater aquaculture system of Assam was undertaken and characterisation of Aeromonads was done through biochemical and molecular studies. Aeromonas hydrophila, Aeromonas veronii and Aeromonas sobria were detected from diseased fishes, with A. hydrophila being the most dominant species (51.64%) followed by A. veronii (21.97%) and A. sobria (18.68%). Pathogenicity studies through experimental infection of Labeo rohita with these isolates resulted in similar clinical signs as those exhibited by the diseased fishes collected from the farm during the outbreak. LD50 (lethal dose) doses estimated for A. hydrophila, A. veronii and A. sobria through probit analysis indicated that 50% mortality was noticed at 10-7.008, 10-8.034 and10-8.213, respectively for A. hydrophila, A. veroniiand A. sobria
with 95% CI (class interval).

Keywords: Aeromonads, Histopathology, Labeo rohita, LD50, Motile aeromonas septicemia (MAS), Pathogenicity

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Author Biographies

Inam Akhtar Hussain, Assistant Professor

Department of Fish Processing Technology, College of Fisheries, AAU, Raha
Kaustubh Bhagabati, Assistant Professor

Department of Aquaculture, College of Fisheries, AAU, Raha
Sangipran Baishya, Assistant Professor

Department of Aquaculture, College of Fisheries, AAU, Raha
Naznin Ahmed, Assistant Professor

Department of Aquatic Animal Health Management, College of Fisheries, AAU, Raha
Hemanta Pokhrel, Assistant Professor

Department of Aquactic Animal Health Management, College of Fisheries, Raha
Dipanka Nath, PhD Scholar

Department of Aquatic Environment Management, College of Fisheries, AAU, Raha

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