Enhancing breeding performance of Osteobramabelangeri (Valenciennes, 1844): Quantitative evaluation of synthetic hormone HATCHME®dosage and water quality parameters
381 / 226
Keywords:
Pengba (Osteobrama belangeri), Induced breeding, HATCHME®, Reproductive parameters, ConservationAbstract
The present study focused on enhancing the breeding performance of Pengba (Osteobrama belangeri) employing the synthetic hormone HATCHME®. Across all trials, twenty-seven brooders were equally allocated and configured at a male-to-female ratio of 2:1. Separation occurred randomly, placing the designated individuals into three distinct groups. Every group was given different dose of the inducing agent. The results demonstrated variations in reproductive performance under the varying experimental conditions. Notably, the best outcome was seen in Treatment 3, where females received 0.6 ml kg-1 body weight and males received 0.3 ml kg-1 body weight. Treatment 1 was ranked ahead of treatment 2, reflecting differences in reproductive outcomes linked to varying hormone doses. Detailed statistical analysis revealed strong correlations between improved breeding success and a combination of factors viz., fish size, hormone doses and key water quality parameters, notably temperature, pH, and dissolved oxygen levels. These important findings provide valuable insights for fish culturists seeking practical and sustainable solutions for successful induced breeding of O. belangeri, simultaneously promoting species conservation and leveraging its lucrative market potential after attaining marketable size.
Keywords: Conservation, HATCHME®, Induced breeding, Pengba, , Reproductive parameters
Downloads
References
References
Angel, J. Raymond Jani., Tiwari, V.K. and Babu, P.P. Suresh. 2015. Captive breeding of a near threatened fish, Pengba, Osteobrama belangeri (Val. 1844) using three different inducing agents. Indian J. Fish., 62(4): 66-70.
APHA Standard methods for the examination of water & wastewater, 21st edition, Eaton, AD, Clesceri, LS, Rice, EW and Greenberg, AE, (Ed.), American Public Health Association, Chapter. 2005; 2:27-61.
Basavaraja, N. 1994. Carp seed production in Karnataka, southern India, with special reference to rearing larvae in reservoir-based pens. NAGA the ICLARM Quarterly, 17 (1): 22-24
Basudha, Ch. And Singh, KH. Rishikanta. 2021. Breeding and culture of Osteobrama belangeri. Indian Journal of Fisheries. 183-191.
Basudha, Ch. And Waikhom, V. 1999. Food and feeding habit of an endemic carp, Osteobrama belangeri (Val. 1844). Indian Journal fish. 46: 71-77.
Behera, B.K., Das, P., Singh, N.S. and Sahu, A.K. 2010. Captive breeding of an endemic medium carp, Osteobrama belangeri (Val. 1844) with Wova-FH in Manipur. J. Aqua. 18: 23-29. DOI: https://doi.org/10.61885/joa.v18.2010.55
Behera, B.K., Meena, D.K., Das, P., Singh, N.S. and Prakashi, S. 2015. Pengba, a prospective species for diversification of carp polyculture; Conservation and future prospects. World Aquaculture: 52-54.
Bhosale, M.M., Suryawanshi, U.A., Somashekar, S.R. and Pai, M., 2020. Induced breeding and embryonic development of rosy barb, Puntius conchonius (Hamilton, 1822) using synthetic hormones under captive condition. Journal of Experimental Zoology India, 23(2).
Biswas, P., Jena, A.K. and Singh, S.K., 2023. Conservation aquaculture of Ompok bimaculatus (Butter catfish), a near threatened catfish in India. Aquaculture and Fisheries, 8(1), pp.1-17. DOI: https://doi.org/10.1016/j.aaf.2022.04.007
Biswas, P., Jena, A.K., Patel, B.A., Arambam, K., Pradhan, A., Singh, K.S., and Singh, D.R. 2019. Recent trends in breeding and seed production of Pengba with special reference to Northeast India. Journal of World Aquaculture.
CAMP 1999. Freshwater fishes of India: Report summary, 1998, Zoo’s Print J., 14:1-27.
Chakrabarti, P. P., Mohapatra, B. C., Ghosh, A., Mandal, S. C., Majhi, D., and Jayasankar, P., 2016. Seed production of Indian major and minor carps in fiberglass reinforced plastic (FRP) hatchery at Bali, a remote Island of Indian Sunderban. International Journal of Fisheries and Aquatic Studies. 4:31-34.
Chanu, T.I., Muralidhar, A., Sharma, Arun. And Karthireddy, S. 2018. Pengba, Osteobrama belangeri; An emerging species for the diversification of fish culture in Andhra Pradesh. Aqua Star: 16-18.
Chaube, R., 2023. An update on induced breeding methods in fish aquaculture and scope for new potential techniques. Frontiers in Aquaculture Biotechnology, pp.55-68. DOI: https://doi.org/10.1016/B978-0-323-91240-2.00006-3
Das, P., Behera, B.K., Meena, D.K., Singh, S.K., Mandal, S.C., Das, S.S., Bhattacharya, B.K. 2016. Comparative efficacy of different inducing agents on breeding performance of near threatened cyprinid Osteobrama belangeri in captivity. Aquaculture Reports. 4: 178-182. DOI: https://doi.org/10.1016/j.aqrep.2016.11.001
Das, P.C., Nayak, A., Sarkar, S., Choudhary, P., Kumari, R. and Mohanty, S., 2022. Growth performance and immune responses of Pengba (Osteobrama belangeri) during high‐density fingerling rearing in biofloc system. Aquaculture Research, 53(17), pp.6378-6388. DOI: https://doi.org/10.1111/are.16111
Das, P.C., Sahoo, P.K., Kamble, S.P., Murmu, K. and Basudha, C. 2020.Compatibility of Pengba, Osteobrama belangeri (Val. 1844) with Indian major carps and evaluation of its ideal incorporation level in carp polyculture system in planes of India. Science, Aquaculture 518, 734845. DOI: https://doi.org/10.1016/j.aquaculture.2019.734845
Das, S.K. and Singh, T.N. 2017. Captive breeding and embryonic development of endangered, Osteobrama belangeri (Val. 1844) under mid-hill condition in Northeast India. Indian Journal of Animal Science. 87: 1548-1550. DOI: https://doi.org/10.56093/ijans.v87i12.79902
Das, S.K., 2004. Evaluation of a new spawning agent, Ovopel in induced breeding of Indian carps. Asian Fisheries Science, 17(4), pp.313-322. DOI: https://doi.org/10.33997/j.afs.2004.17.4.004
Debnath, D. and Yengkokpam, S., 2024. Floodplain Wetlands of Ganga-Brahmaputra River Basins: Importance as Fishery Resources and Their Conservation Needs. In Aquaculture and Conservation of Inland Coldwater Fishes (pp. 401-424). Singapore: Springer Nature Singapore. DOI: https://doi.org/10.1007/978-981-97-1790-3_22
Deepti, M., Meitei, M.M., Ngasotter, S. and Mahendrajit, A. 2023. Pengba, Osteobrama belangeri – a candidate species for diversification in aquaculture. Aquaculture.
Devi, G.A., Devi, G.S., Singh, O.B., Munilkumar, S. and Reddy, A.K. 2009. Induced spawning and Hatching of Osteobrama belangeri (Val. 1844) Using Ovatide, an Ovulating Agent. Asian Fisheries Science 22(4):1107-1115. DOI: https://doi.org/10.33997/j.afs.2009.22.4.005
Ghosh, A.K., Biswas, S., Sarder, L., Sabbir, W. and Rahaman, S.M.B., 2012. Induced breeding, embryonic and larval development of Koi carp (Cyprinus carpio) in Khulna, Bangladesh. Mesopot. J. Mar. Sci, 27(1), pp.1-14. DOI: https://doi.org/10.58629/mjms.v27i1.167
Gorre, D. and Chari, T.J., 2023. Induced breeding of common carp (Cyprinus carpio) by using synthetic hormones. DOI: https://doi.org/10.22271/fish.2023.v11.i4b.2834
Gupta SD, Mohapatra BC, Routray P, Sahoo SK, Verma DK, Sarangi N. Text Book of Breeding and Hatchery Management of Carps. Narendra Publishing House, Delhi, 2008, 1-163.
Gurjar, U.R., Takar, S., Bunkar, K., Mohan, D. and Pandey, N.N., 2024. Coldwater Fish Diversity: Issues and Sustainable Management in India. In Coldwater Fisheries and Aquaculture Management (pp. 179-193). Apple Academic Press. DOI: https://doi.org/10.1201/9781003369905-6
Iqbal, S., Atique, U., Abbas, F., Ahmad, S., and Haider, M. S., 2021. Impact of temperature variations on breeding behaviour of Cirrhinus mrigala during Induced Spawning. Pakistan Journal of Zoology., pp 1-6.
Islam, M. M., and Amin, A. A. M., 2016. The induced breeding of common carps (Cyprinus carpio) in Bangladesh. Indian Journal of Science. 23 (84): 619- 632.
IUCN, 2010. The IUCN Red List of Threatened Species. http://dx.doi.org/10.2305/IUCN.UK.2010-4.RLTS.T168218A6467894.en. DOI: https://doi.org/10.2305/IUCN.UK.2010-4.RLTS.T168218A6467894.en
Jayasankar, P., Mohanta, K.N. and Ferosekhan, S., 2018. Freshwater aquaculture in India. Aquaculture in India (Edited by: Tripathi SD, Lakra WS, Chadha NK), pp.23-49.
Khan M. K., Shakir H. A., Ashraf M. and Ahmad Z. 2006. Induced spawning of Labeo rohita using synthetic hormone. J. Zool., Vol. 21 (1-2), pp. 67-72.
Khan, J. and Ali, F., 2021. Effect of pituitary gland extract (PGE) and Ovaprim on induced breeding in Rohu (Labeo rohita) and grass carp (Ctenopharyngodon idella). International Journal of Biology Sciences, 3(2), pp.12-16. DOI: https://doi.org/10.33545/26649926.2021.v3.i2a.30
Kumar, A., Ravi, C., Chanu, T.I., Mishra, S., Kumar, U. and Das, P.C., 2017. Effect of different weaning strategies on survival and growth of endangered fish Pengba, Osteobrama belangeri (Valenciennes, 1844) larvae. International Journal of Pure & Applied Bioscience (IJPAB), 5(5), pp.951-957. DOI: https://doi.org/10.18782/2320-7051.5153
Laskar, B.A., Banerjee, D., Chung, S., Kim, H.W., Kim, A.R. and Kundu, S., 2024. Integrative Taxonomy Clarifies the Historical Flaws in the Systematics and Distributions of Two Osteobrama fishes (Cypriniformes: Cyprinidae) in India. Fishes, 9(3), p.87. DOI: https://doi.org/10.3390/fishes9030087
Menon, A.G.K. 2004. Threatened fishes of India and their conservation. Zool. Soc. India, 170 pp.
Mohapatra, B. C., Mohanta, K. N., and Majhi, D., 2018. Indian major carps seed production through induced breeding in FRP hatchery at Bisoi, Mayurbhanj district, Odisha, India. International Journal of Fisheries and aquatic Studies. 6 (4): 492- 496.
Nazir, S., Khan, N., Azmat, H., Naveed, S., Ramzan, M.M. and Davies, S.J., 2023. Efficacy of various concentrations of synthetic hormones on the induced breeding of Channa marulius (Sole). Journal of the World Aquaculture Society, 54(1), pp.143-155. DOI: https://doi.org/10.1111/jwas.12908
Rajts, F., Dubey, S.K., Belton, B., Panemangalore, A.P. and Thilsted, S.H., 2023. Current state of knowledge on induced breeding of nutrient-rich small indigenous fish species. (Val.) from Manipur. International Journal of Fisheries and Aquaculture, 3(7), pp.142-144.
Rakkannan, G., Kashyap, S., Debnath, B., Sahoo, D., Singh, Y.S., Patel, A.B. and Priyadarshi, H., 2023. Microsatellite maker analysis reveals the low genetic diversity in an endangered fish, Osteobrama belangeri in the Indo-Myanmar region. DOI: https://doi.org/10.21203/rs.3.rs-2680847/v1
Ramesh, R., Dube, K., Reddy, A. K., Prakash, C., Tiwari, V. K., Rangacharyulu, P.V., Venkateshwarlu, G. (2014). Growth and survival of Pengba, Osteobrama belangeri (Val.) larvae in response to co-feeding with live feed and microparticulate diet. Eco. Env. & Cons. 20 (4), 1715-1721.
Rath, S.C., Sarkar, S.K., Gupta, S.D. and Sarangi, N., 2007. Comparative account of induced breeding of Indian major carps with Ovaprim, Ovatide, Wova-FH and carp pituitary extract. The Indian Journal of Animal Sciences, 77(10).
Reddy P.V.G.K. 2000. Captive breeding of Osteobrama belangeri (Val.)- A threatened food species. In: Fish Biodiversity of North East India. (ed. A.G. Ponniah and U.K. Sarkar), pp 122-123. NATP Publ.2. National Bureau of Fish Genetics Re-sources, Lucknow, India.
Sahoo, L., Behera, B.K., Panda, D., Parhi, J., Debnath, C., Mallik, S.K. and Roul, S.K., 2023. Fisheries and aquaculture. In Trajectory of 75 years of Indian Agriculture after Independence (pp. 313-330). Singapore: Springer Nature Singapore.
Sahoo, L., Behera, B.K., Panda, D., Parhi, J., Debnath, C., Mallik, S.K. and Roul, S.K., Fisheries and Aquaculture 13. Trajectory of 75 years of Indian Agriculture after Independence, p.313. DOI: https://doi.org/10.1007/978-981-19-7997-2_13
Sarma, D. and Mohan, D., 2024. Himalayan Fishery Resources: Treasury of Coldwater Fishes for Sustainable Aquaculture. In Aquaculture and Conservation of Inland Coldwater Fishes (pp. 1-26). Singapore: Springer Nature Singapore. DOI: https://doi.org/10.1007/978-981-97-1790-3_1
Shashikumar, O. 1991. Studies on the biology and nutritive value of Osteobrama belangeri (Val.) Ph.D. Thesis, Manipur University, India.
Suresh, V.R. 2000. Food habits and composite culture potential of an endangered medium carp Osteobrama belangeri (Val.) in Manipur.
Surnar, S.R., Kamble, A.D., Walse, N.S., Sharma, O.P. and Saini, V.P., 2015. Hormone administration with induced spawning of Indian major carp. International Journal of Fisheries and Aquatic Studies, 3(1), pp.01-04.
Tiwana, G.S. and Raman, S., 2012. An Economically Viable Approach for Induced Breeding of Labeo rohita by Ovatide, Ovaprim and Carp Pituitary Extract. IOSR Journal of Agriculture and Veterinary Science, 1(1), pp.30-32. DOI: https://doi.org/10.9790/2380-0113032
Waikhom, V. 2010. Osteobrama belangeri. In: IUCN Red List of Threatened Species, version 2012.1.
Zamri, A.S., Zulperi, Z., Esa, Y. and Syukri, F., 2022. Hormone Application for Artificial Breeding Towards Sustainable Aquaculture–A Review. Pertanika Journal of Tropical Agricultural Science, 45(4). DOI: https://doi.org/10.47836/pjtas.45.4.11
Downloads
Submitted
Published
Issue
Section
License
Copyright (c) 2025 Indian Journal of Fisheries

This work is licensed under a Creative Commons Attribution-NoDerivatives 4.0 International License.
The copyright of the articles published in Indian Journal of Fisheries vests with the Indian Council of Agricultural Research, who has the right to enter into any agreement with any organization in India or abroad engaged in reprography, photocopying, storage and dissemination of information contained in these journals. The Council has no objection in using the material, provided the information is being utilized for academic purpose but not for commercial use. Due credit line should be given to the ICAR where information will be utilized.