Isolation and characterisation of Vibrio alginolyticus lytic bacteriophage φVa-1 from brackishwater clam Meretrix meretrix in India
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Authors
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E. C. Abhilash
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S. V. Alavandi
ICAR-National Referral Laboratory for Brackishwater Aquatic Animal Diseases,
Aquatic Animal Health and Environment Division,
Central Institute of Brackishwater Aquaculture (ICAR-CIBA),
#75, Santhome High Road, Raja Annamalai Puram,
Chennai, Tamil Nadu 600028
Abstract
The Gram negative bacterium Vibrio alginolyticus is generally found in marine and brackishwater systems. A lytic bacteriophage capable of specifically infecting V. alginolyticus was isolated from the brackishwater clam (Meretrix meretrix) using agar overlay technique. The phage produced plaques 3 mm in dia, which increased to 5 mm overnight on tryptone soya agar (TSA) plates and the optimum temperature and pH was found to be 32°C and 7.5 respectively. The phage was designated as φVa-1 and nucleic acid characterisation confirmed that the phage has double stranded DNA. Transmission electron microscopic observations revealed that the bacteriophage had hexagonal structure with a long contractile tail and
the phage was found to belong to the family Myoviridae.
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