Cryoconservation of some wild species of Musa L.
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Authors
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Anuradha Agrawal
ICAR-National Bureau of Plant Genetic Resources (NBPGR), Pusa Campus, New Delhi 110 012
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Smriti Verma
ICAR-National Bureau of Plant Genetic Resources (NBPGR), Pusa Campus, New Delhi 110 012
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Namrata Sharma
ICAR-National Bureau of Plant Genetic Resources (NBPGR), Pusa Campus, New Delhi 110 012
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Priyanka Vijay
ICAR-National Bureau of Plant Genetic Resources (NBPGR), Pusa Campus, New Delhi 110 012
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D. P. S. Meena
ICAR-National Bureau of Plant Genetic Resources (NBPGR), Pusa Campus, New Delhi 110 012
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R.K. Tyagi
ICAR-National Bureau of Plant Genetic Resources (NBPGR), Pusa Campus, New Delhi 110 012
Abstract
The present work was undertaken to devise suitable medium for induction of proliferating meristems in five wild species of Musa (M. acuminata, M. balbisiana, M. basjo, M. jackeyi and M. textilis) and their subsequent cryopreservation by modification of the standard droplet
vitrification protocol. It was found that BAP (100 ìM) alone was not sufficient to induce high proliferation rates and inclusion of TDZ (1-10 ìM) was essential. The duration of pre-growth desiccation (9-14 d) and dehydration with cryoprotectant (PVS2, 90-120 min) varied with each
species.The optimized protocol for each species yielded 46-58% shoot recovery after cryopreservation. In terms of ease of explant generation, percentage shoot recovery and plantlet formation, M. jackeyi > M. basjoo > M. textilis> M. acuminata>M. balbisiana.