Pyramiding of leaf rust resistance genes in bread wheat variety DWR 162 through marker assisted backcrossing
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Authors
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Arati Yadawad
Dr. Sanjaya Rajaram Wheat Laboratory, Main Agricultural Research Station, University of Agricultural Sciences, Dharwad 580 005
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Anil Gadpale
Dr. Sanjaya Rajaram Wheat Laboratory, Main Agricultural Research Station, University of Agricultural Sciences, Dharwad 580 005
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R. R. Hanchinal
Dr. Sanjaya Rajaram Wheat Laboratory, Main Agricultural Research Station, University of Agricultural Sciences, Dharwad 580 005
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H. L. Nadaf
Dr. Sanjaya Rajaram Wheat Laboratory, Main Agricultural Research Station, University of Agricultural Sciences, Dharwad 580 005
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S. A. Desai
Dr. Sanjaya Rajaram Wheat Laboratory, Main Agricultural Research Station, University of Agricultural Sciences, Dharwad 580 005
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Suma Biradar
Dr. Sanjaya Rajaram Wheat Laboratory, Main Agricultural Research Station, University of Agricultural Sciences, Dharwad 580 005
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V. Rudra Naik
Dr. Sanjaya Rajaram Wheat Laboratory, Main Agricultural Research Station, University of Agricultural Sciences, Dharwad 580 005
Abstract
A popular widely grown but leaf rust susceptible Indian
bread wheat variety was pyramided with two leaf rust
resistance genes, Lr24 and Lr28 by Marker Assisted
Backcrossing (MABC). The near isogenic line of PBW 343
introgressed with Lr24 and Lr28 was used as a donor.
The MABC approach employed one SSR marker, Xwmc313
and one SCAR marker SCS421 with its locus linked to
Lr28 and two SCAR markers, SCS719 and SCS 1302 linked
to Lr24 for foreground selection to select plants carrying
Lr24 and Lr28 genes. Marker assisted background selection
in BC2F2to recover the genome of recipient parent involving
42 polymorphic SSR markers dispersed throughout the
genome facilitated mean recurrent parent genome (RPG)
recovery of 80.3% with a range from 67.5-94.7%. BC2F2
plants were characterized for DUS (Distinctiveness
Uniformity Stability) for phenotypic identification of plants
that mostly resemble DWR 162 variety. Field evaluation of
BC2F2 plants for yield and its component traits to assess
the effect of these two genes in the background of DWR
162 revealed high yield potential of selected plants along
with leaf rust resistance and no yield penalty was apparent.
This study successfully demonstrate the practical utility of
MABC in wheat rust resistance breeding for developing
resistant lines in the background of any elite and popular
wheat cultivar with relatively higher speed and precision.