Molecular marker-based validation of blast resistance gene Pi54 and identification of potential donors in temperate high altitude rice (Oryza sativa L.)
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Authors
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Gazala H. Khan
Division of Biotechnology, Sher-e-Kashmir University of Agricultural Sciences and Technology
of Kashmir, Jammu and Kashmir 191 121
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S. Najeeb
Mountain Research Centre for Field Crops, Khudwani; Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Jammu and Kashmir 191 121
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Zahoor A. Bhat
Division of Genetics and Plant Breeding, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Jammu and Kashmir 191 121
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B. A. Padder
Division of Plant Pathology; Sher-e-Kashmir University of Agricultural Sciences and Technology
of Kashmir, Jammu and Kashmir 191 121
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G. A. Parray
Mountain Research Centre for Field Crops, Khudwani; Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Jammu and Kashmir 191 121
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Shafiq A. Wani
Division of Biotechnology, Sher-e-Kashmir University of Agricultural Sciences and Technology
of Kashmir, Jammu and Kashmir 191 121
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Asif B. Shikari
Division of Biotechnology, Sher-e-Kashmir University of Agricultural Sciences and Technology
of Kashmir, Jammu and Kashmir 191 121
Abstract
More than 100 genes have been reported to impart resistance
against rice blast, however, not all are equally effective.
Their effectiveness relies on factors such as the diversity in
pathogen races prevailing in a certain area, rate of pathogen
evolution, genetic background of a host and few others.
Pi54 is a major gene showing resistance to Magnaporthe
populations in North-west Himalayas. In search of novel
temperate donors suitable to high altitudes, a set of
germplasm was screened using gene based markers for
Pi54. Eighty three exotic and indigenous germplasm lines
were genotyped using gene based markers and also
validated for disease reaction using Pi54 gene specific
isolate namely, Mo-nwi-kash-32. Nine out of 83 germplasm
lines amplified resistance specific alleles with both the
markers Pi54 MAS and Pikh-STS. All these lines expressed
resistance against the said diagnostic isolate, thereby
validating the possible presence of gene in the lines. Further
validation using more number of isolates and sequence
analysis will help in mining useful alleles for this gene