Studies on involvement of Wrinkled1 transcription factor in the development of extra-long staple in cotton
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Authors
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Uzma Qaisar
School of Biological Sciences, University of the Punjab, Lahore 54590, Pakistan
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Fozia Akhtar
School of Biological Sciences, University of the Punjab, Lahore 54590, Pakistan
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Muhammed Azeem
Department of Botany, Government College University, Faisalabad
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Samina Yousaf
Botany Department, University of the Punjab, Lahore 54590, Pakistan
Abstract
In the present investigation, fiber transcriptome of
Gossypium hirsutum producing short-fiber (JKC703,
JKC737, JKC783) and long-fiber (JKC725, JKC777,
Guazuncho 2) was compared with the transcriptome of
extra-long fiber producing Gossypium barbadense (VH8-
4602). By meta-analysis, we identified 1431 differentially
expressed genes among the genotypes of different fibre
lengths. Of these, 574 genes were up-regulated while 844
were down regulated in G. barbadense as compared to G.
hirsutum. Expression of 5 genes related to fiber
development was tested in local germplasm of G.
barbadense (Bar14/5) and used G. hirsutum (MNH 886) using
real-time RT-PCR. G. arboreum (FDH786) was as a sample
with unknown expression. Expression pattern of an
ethylene responsive transcription factor wrinkeled-1 (wri1),
a vacuolar processing enzyme (vpe) and eceriferum3 (cer3)
gene showed close association with fiber lengths. Moreover,
cotton wri1 expressed in apical-meristem and bolls and
showed significantly higher expression in fiber of G.
barbadense as compared to G. hirsutum and G. arboreum
during development. Level of Transcription of wri1 was
enhanced in fiber during ball development while it remained
unchanged in fiber-less seeds.