Effect of dandelion leaves and fenugreek seeds with or without enzyme addition on performance and blood biochemistry of broiler chicken, and evaluation of their in vitro antibacterial activity
DOI:
https://doi.org/10.56093/ijans.v85i11.53302Keywords:
Antibacterial, Blood biochemistry, Broiler, Dandelion, Fenugreek, PerformanceAbstract
The study was conducted to evaluate effect of dietary supplementation of locally available herbs dandelion leaves and fenugreek seeds with or without enzyme treatment in broiler chicken. Day-old commercial broiler chicks (273) were reared together until 7 days of age and on 7th day, and individually weighed, and distributed randomly into 7 groups of 3 replicates with 13 chicks each. Birds in the control group were fed diets without additives (T1). The other 6 treatment groups were fed the basal diet supplemented with 0.5% dandelion leaves (T2), 1% fenugreek seeds (T3), combination of 0.5% dandelion leaves and 1% fenugreek seeds (T4), enzyme treated dandelion leaves 0.5% (T5), enzyme treated fenugreek seeds 1% (T6), and combination of enzyme treated dandelion leaves (0.5%) and (1%) fenugreek seeds (T7). The results revealed a significant improvement in the live body weight of broiler chicken fed diets supplemented with dandelion leaves and fenugreek seeds with or without enzyme treatment (T5, T6 and T7) when compared with the control group. A significant improvement in the live body weight and body weight gain was obtained in the said groups from 3 to 6 weeks of age. Highest live body weight was observed in the group fed combination of enzyme treated dandelion leaves and fenugreek seeds (T7) followed by 1892.62g±5.11 in enzyme treated dandelion group (T6). Among the groups fed dandelion leaves and fenugreek seeds without enzyme, highest body weight was achieved in the group fed 1% combination of dandelion leaves and fenugreek seeds in the diet (T4), followed by the group fed fenugreek alone (T3). Fenugreek seeds showed better results in terms of growth of birds than dandelion leaves. Highest cumulative feed consumption was recorded in all the groups in which 1% fenugreek seeds were supplemented when compared with the control group. However, a nonsignificant effect was observed in the dandelion groups compared to control in terms of cumulative feed consumption. The feed conversion ratio (FCR) was significantly improved in the birds fed diets containing either raw or enzyme treated dandelion leaves and fenugreek seeds, alone or in combination, when compared with the control group. Among the different treatment groups, best FCR was observed in the group fed combination of enzyme treated dandelion leaves and fenugreek seeds (T7), followed by enzyme treated dandelion group. The mean values of serum glucose and cholesterol significantly reduced among different treatment groups compared to control, the highest reduction being observed in the groups fed combination of dandelion leaves and fenugreek seeds with or without enzyme addition. Further, the in vitro study revealed that both the dandelion leaves and fenugreek seeds have antibacterial properties with zone of inhibition as 2 mm from dandelion leaves and 2.1 mm from fenugreek seeds for the concentration of 0.5 mg/ml and 0.05 mg/ml of extract against Escherichia coli on the Mueller Hinton Agar, respectively.References
Abbas R J. 2010. Effect of using fenugreek, parsley and sweet basil seeds as feed additives on the performance of broiler chickens. International Journal of Poultry Science 9 (7): 278– 82.
Aksa S B, Alloui N and Alloui M N. 2012. Utilization of fenugreek (Trigonella foenum- graecum) as growth promoter for broiler chickens. Journal of World’s Poultry Reseach 2 (2): 25–27.
Al-Harthi M A. 2006. Impact of supplemental feed enzymes, condiments mixture or their combination on broiler performance, nutrients digestibility and plasma constituents. International Journal of Poultry Science 5 (8): 764–71.
Ali A, Hassan A K and Nasreen N. 1995. Characterization of the hypoglycemic effects of Trigonella foenum - graecum seed. Journal of Planta Medica 61: 358–60.
Ali A H H and Ismail Z S H. 2012. Some physiological changes related to Fenugreek seeds supplementation to broiler chick’s diet. Egyptian Journal of Nutrition and Feeds. 15 (1): 95– 104.
Al-Kassie G A M, Al-Jumaa Y M F and Jameel Y J. 2008. Effect of probiotic (Aspergillus niger) and prebiotic (Taraxacum officinale) on blood picture and biochemical properties of broiler chicks. International Journal of Poultry Science 7 (12): 1182– 84.
AOAC. 2005a. Official Methods of Analysis. 18th edn. Association of Official Analytical Chemists. North Frederick Avenue, Gaithersburg, Meryland, USA.
Bansal G R, Singh V P and Sachan N. 2012. Carcass characteristics of commercial broiler chicks as affected by enzyme supplementation. International Journal for Agro Veterinary and Medical Sciences 6 (6): 413–17.
Bash E, Ulbricht C, Kuo G and Smith M. 2003. Therapeutic applications of fenugreek. Alternative Medicine Review 8: 20– 27.
BIS. 1992. Poultry Feeds Specifications. Bureau of Indian standards, Manak Bhawan, 9 Bahadurshah Zafar marg, New- Delhi.
Broca C, Manteghetti M and Gross R. 2000. 4- Hydroxyisoleucine: effects of synthetic and natural analogues on insulin secretion. European Journal of Pharmacology 390: 339–45.
Clinical and Laboratory Standards (CLSI). 2007. Methods for Antimicrobial Dilution And Disk Susceptibility Testing of Infrequently Isolated or Fastidious Bacteria. Approved guideline M45-A Clinical and Laboratory Standards Institute, Wayne, PA.
Dash B K, Sultana S and Sultana N. 2011. Antibacterial activities of methanol and acetone extracts of fenugreek (Trigonella foenum) and coriander (Coriandrum sativum). Life Sciences and Medicine Research 27: 65–72.
Duncan D B. 1955. Multiple range and multiple F test. Biometrics 11: 1–42.
Duru M, Zeynep E and Asuman D. 2013. Effect of seed powder of a herbal legume Fenugreek (Trigonella foenum-graceum L.) on growth performance, body components, digestive parts, and blood parameters of broiler chicks. Pakistan Journal of Zoology 45 (4): 1007–14.
Eman A. 2011. Pathological and biochemical studies on the effect of Trigonella foenum-graecum and Lupinus termis in alloxan induced diabetic rats. World Applied Sciences Journal 12 (10): 1839–50.
El-Gendi G M, Ismail F A and El-Aggoury S M. 1994. Effect of Cocci-Nel and Lomoton dietary supplementation as herbal growth promoters on productive performance of broiler. Annals of Agriculture Science Moshtohor 32 (4): 1511–28.
Fulton R M, Nersessian B N and Reed W M. 2002. Prevention of Salmonella enteritidis infection in commercial ducklings by oral chicken egg-derived antibody alone or in combination with probiotics. International Journal of Poultry Science 81 (1): 34–40.
Galib A M, Al-Kassi A and Noor M W. 2010. A comparative study on diet supplementation with a mixture of herbal plants and dandelion as a source of prebiotics on the performance of broilers. Pakistan Journal of Nutrition 9 (1): 67–71.
Hashemi S R, Zulkifli I, Hair-Bejo M, Farida A and Somchit M N. 2008. Acute toxicity study and phytochemical screening of selected herbal aqueous extract in broiler chickens. International Journal of Pharmacology 4: 352–60.
Hashemi S R and Davoodi H. 2010. Phytogenics as new class of feed additive in poultry industry. Journal of Animal and Veterinary Advances 9 (17): 2295–304.
Hernandez A I, Madrid J and Garcia V. 2004. Influence of two plant extracts on broiler performances, digestibility and digestive organs size. International Journal of Poultry Science 83: 169–74.
Husain M. 2001. Geography of Jammu and Kashmir. Rajesh Publication, New Delhi, India, pp. 28.
Jackson B S. 1982. The lowly dandelion deserves more respect. Canadian Geographic Magazine 102: 54–59.
Jassim M N, Safanah A F and Omar M N. 2012. Identification of dandelion Taraxacum officinale leaves components and study its extracts effect on different microorganisms. Journal of Al- Nahrain University 15 (3): 7–14.
Khan S H and Siddique B. 2006. Influence of enzymes on performance of broilers fed sunflower-corn based diets. Pakistan Veterinary Journal 26 (3): 109–14.
Langhout P. 2000. New additives for broiler chicken in vivo. World Poultry Journal 16 (6): 22–27.
Lazaro R, Garcia M, Medel P and Mateos G G. 2003. Influence of enzymes on performance and digestive parameters of broilers fed rye - based diets. Poultry Science 82 (7): 132–40.
Lin J, Opoku A R, Geheeb-Keller M, Hutchings A D and Staden J V. 1999. Preliminary screening of some traditional zulu medicinal plants for anti- inflammatory and anti-microbial activities. Journal of Ethnopharmacology 68: 267– 74.
Luo D, Fengxia Y, Xiaojun Y and Junhu Y. 2009. Effects of xylanase on performance, blood parameters, intestinal morphology, microflora and digestive enzyme activities of broilers fed wheat-based diets. Asian-Australasian Journal of Animal Science 22 (9): 1288–95.
Magda E E. 2012 Effect of dietary Fenugreek seeds (Trigonella foenum) as natural feed addition on broiler chicks performance. Journal of Science and Technology 13 (2): 27–31.
Malik H A, Khuroo A A and Dar G H. 2011. Ethonomedicinal Uses of some plants in the Kashmir Himalayas. Indian Journal of Traditional Knowledge 10 (2):362–66.
Mamoun T, Mukhtar A and Mohamed H T. 2014. Effect of fenugreek seed powder on the performance, carcass characteristics and some blood serum attributes. Advanced Research of Agriculture and Veterinary Science 1 (1): 6–11.
Meghwal M and Goswami T K. 2012. A review on the functional properties, nutritional content, medicinal utilization and potential application of fenugreek. Journal of Food Process Technology 3 (9): 169–78.
Michael D and Kumawat D. 2003. Legend and archeology of fenugreek, constitutions and modern applications of fenugreek seeds. International Symposium, USA, pp. 41–42.
Mir M A, Sawhney S S and Jassal M M S. 2013. Qualitative and quantitative analysis of phytochemicals of Taraxacum officinale. Wudpecker Journal of Pharmacy and Pharmocology 2 (1): 1–5.
Mullaicharam A R, Geetali D and Uma M. 2013. Medicinal values of fenugreek - A review. Research Journal of Pharmaceutical, Biological and Chemical Sciences 4 (1): 1304.
Murray R K, Granner D K, Mayes P A and Rodwell V W. 1991. The Text Book of Harpers Biochemistry. 22nd edn. Applecton and Large, Norwalk, Connecticut/Loss Altos, California.
Park C, Yusi Z and Youngsun S. 2007. Hepatoprotective effect of dandelion (Taraxacum officinale) against acute liver injury induced by carbon tetrachloride in Sprague-Dawley rats. Journal of Federation of American Societies for Experimental Biology 21 (1): 862.
Petit P, Sauvair Y and Hillaire D. 1995. Steroid saponins from fenugreek seeds: Extraction, purification, and pharmacological investigation on feeding behavior and plasma cholesterol. Steroids 60: 674–80.
Rabia J A. 2010. Effect of using fenugreek, parsley and sweet basil seeds as feed additives on the performance of broiler chickens. International Journal of Poultry Science 9 (3): 278– 82.
Raziq F, Khan S, Naila C and Asad S. 2012. Effect of water based infusion of Lycium, Aloe barbedensis, Pimpinella anisum, Berberis Trigonella foenum-graecum and Allium sativum on the performance of broiler chicks. Pakistan Veterinary Journal 32 (4): 593–96.
Schryver T. 2002. Fenugreek. Total Health 24: 42–44.
Shakmak S. 2003. ‘Studies on Improvement of productive performance in poultry.’ M.Sc. Thesis submitted to Mansoura University, Egypt.
Sundu B, Kumar A and Dingle J. 2006 Response of broiler chicks fed increasing levels of copra meal and enzymes. International Journal of Poultry Science 5 (1): 13–18.
Tabassum N, Shah M Y and Qazi M A. 2010. Prophlactic activity of extract of Taraxacum officinale against hepatocellular injury induced in mice. Pharmacologyonline 2: 344–52.
Weerasingha A S and Atapattu N S B M. 2013. Effects of Fenugreek (Trigonella foenum-graecum L.) seed powder on growth performance, visceral organ weight, serum cholesterol levels and the nitrogen retention of broiler chicken. Tropical Agricultural Research 24 (3): 289–95.
Wenk C. 2003. Herbs and botanicals as feed additives in monogastric animals. Asian-Australasian Journal of Animal Science 16 (2): 282–89.
Yarnell E and Abascal J D. 2009. Dandelion (Taraxacum officinale and T. mongolicum). Integrative Medicine 8 (2): 310–16.
Yatoo M A, Sharma R K and Khan N. 2012. Effect of fenugreek and black cumin seeds as feed additives on blood biochemical profile and performance of broilers. Indian Journal of Animal Nutrition 29 (2): 174–78.
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