In vitro evaluation of Dichanthium annulatum (Marvel grass) grass hay diets supplemented with browse foliage

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  • SULTAN SINGH Principal Scientist, Indian Grassland and Fodder Research Institute, Jhansi, Uttar Pradesh 284 003 India
  • B B SINGH Ex-SRF, Plant Animal Relationship Division, Indian Grassland and Fodder Research Institute, Jhansi, Uttar Pradesh 284 003 India


Dicanthium annulatum, Gas production, In vitro evaluation, Shrubs/Tree foliage


In present study 20 Dichanthium annulatum-DA grass based diets formulated with browse foliage from 5 tree leaves (Hardwickia binata-HB, Albizia lebbek-AL, Grewia optiva-GO, Anogeissus pendulla-AP and Leucaena leucocephala-LL) and five shrubs (Dichrostachys cineria-DC, Securengia virosa-SV, Zizyphus xylophyrus-ZX, Helictris isora-HI and Acacia catechu-AC) in 50:50 and 75: 25 ratios were assessed for in vitro nutrients degradation, metabolites and gas production using sheep and goat rumen liquor. CP, NDF, ADF and cellulose contents differ significantly (P<0.05) amongst the diets. CP contents were higher in DA: LL (9.55) and DA: AL (8.58) diets and lowest in DA: HB diet (4.87%), while NDF, ADF and cellulose contents were higher in DA: AL (62.86, 42.09 and 32.82%) and lowest in DA: LL (55.15, 31.75 and 24.24 %). Mean values of DM, CP, NDF and ADF degradability varied significantly across diets in rumen inoculum of both species. DA: LL, DA: SV and DA: GO diets had higher DM, CP, NDF and ADF degradability in rumen liquor of both animal species. TVFA, total-N and ammonia-N contents were higher in sheep and goat from DA: LL, DA: SV and DA: GO diets. Sheep and goat rumen liquor had higher mean total-N contents at 50:50 ration of grass-foliage diets (45.36 and 47.57) than at 75: 25 ratio (36.00 and 36.96 mg/dl), respectively. Mean values of IVDMD (%), gas (ml/g) and partition factor differed across the diets in both species. Diets mean values for IVDMD and gas tended to be more for goats (167.50 and 54.13) than sheep (142.99 ml/g and 52.25%), while partition factor values tended to be more for sheep (3.58) than goats (3.01). DA: LL, DA: GO and DA: SV diets had higher gas and IVDMD in rumen liquor of both species and lowest for DA: DC diet. Results showed that DA: LL, DA: GO and DA: SV have higher nutrients digestibility with higher metabolites concentration on fermentation in sheep and goat rumen liquor.


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A O A C. 1995. Official Methods of Analysis. 13th edn. Association of Official Analytical Chemists, Washington D C.

Alemu B, Animut G and Tolera A. 2014. Effect of Millettia ferruginea (Birbra) foliage supplementation on feed intake, digestibility, body weight change and carcass characteristics of Washera sheep fed natural pasture grass hay basal diet. Springer Plus 3: 50 DOI:

Aregheore E M. 2007. Voluntary intake, nutrient digestibility and nutritive value of foliage of fluted pumpkin (Talfairia occidentialis) - haylage mixtures by goats. Livestock Research and Rural Development 19 (4):

Aregheore E M and Perera D. 2004. Effect of supplementation of a basal diet of maize stover withErythrina variegate, Gliricidia sepiumor Leucaena leucocephala on feed intake digestibility by goats. Tropical animal Health and Production 36:175–89. DOI:

Barakat N A M, Laudadio V, CazzatoE and Tufarelli V. 2013. Potential contribution of retamaraetam (Forssk.) Webb & Berthel as a forage shrub in Sinai, Egypt. Arid Land Research Management 27: 257–71. DOI:

Blümmel M and Lebzien P. 2001. Predicting ruminal microbial efficiencies of dairy ration by in vitro techniques. Livestock Production Science 68: 107–17. DOI:

Briggs P K, Hogen J P, Reid R I. 1957. Effect of volatile fatty acids, lactic acid and ammonia on rumen pH in sheep. Australian Journal of Agriculture Research 8: 674. DOI:

Convey E J. 1957. Micro-diffusion Analysis and Volumetric Error. 4th edn. Crossby Lockwood and Sons Lords, England.

Cook B G, Pengelly B C, Brown, S D, Donnelly J L, Eagles D A, Franco M A, Hanson J, Mullen B F, Partridge I J, Peters M, and Schultze-Kraft R. 2005. Tropical Forages. CSIRO, DPI&F (Qld), CIAT and ILRI, Brisbane, Australia.

FAO, 2010. Grassland Index. A searchable catalogue of grass and forage legumes. FAO.

Gebregiorgis F, Negesse T and Nurfeta A. 2012. Feed intake and utilization in sheep fed graded levels of dried moringa (Moringa stenopetala) leaf as a supplement to Rhodes grass hay. Tropical Animal Health and Production 44 (3): 511–17. DOI:

Hindrichsen I K, Osuji P O, Odenyo A A, Madsen J and Hvelplund T. 2004. Effect of supplementation of maize stover with foliage of various tropical multipurpose trees and Lablab purpureus on intake, rumen fermentation, digesta kinetics and microbial protein supply of sheep. Animal Feed Science and Technology 113:83–96. DOI:

Isah O A, Oguntuyo S A, Dawodu R O, Diya O O, Afolabi M O and Omoniyi L A. 2013. Feed utilization, rumen parameters, and microbial profile of goats fed different tropical browse plants with Pennisetum purpureumas basal diet. Pacific Journal of Science and Technology 14 (1): 397–405.

Manaye T, Tolera A and Zewdu T. 2009. Feed intake, digestibility and body weight gain of sheep fed Napier grass mixed with different levels of Sesbania sesban. Livestock Science 122: 24–29. DOI:

McKenzie H A and Wallace H S. 1954. The kjeldahl determination of nitrogen. Australian Journal of Chemistry 7: 55–61 DOI:

Molina E A, Garcia A I M and Aguilera J F. 2000. A comparative study of nutrient digestibility, kinetics of degradation and passage and rumen fermentation pattern in goats and sheep offered good quality diets. Livestock Production Science 64: 215–23. DOI:

Njidda A A, Ikhimioya I and Isidahomen C E. 2013. In situ crude protein degradation and mineral composition of browse forages of semi-arid Nigeria. International Journal of Agriculture and Bioscience 2(5): 286–96.

Olafadehan O A. 2013. Feeding value of Pterocarpus erinaceus for growing goats. Animal Feed Science and Technology 185:1–8. DOI:

Ondiek J O, Ogore P B, Shakala E K and Kaburu G M. 2013. Feed intake, digestibility and performance of growing small East African goats offered maize (Zea mays) stover supplemented with Balanites aegyptiaca and Acacia tortilis leaf forages. Basic Research. Journal of Agriculture. Science and Review 2 (1): 21–26.

Osakwe I I and Steingass H. 2013. Ruminal fermentation and nutrient digestion in West African Dwarf (WAD) sheep fed Dialium guineense supplemental diets. Proceedings of Conference on International Research on Food Security, Natural Resource Management and Rural Development. (17– 19 Sep 2013), Stuttgart, Germany.

Osakwe I I and Udeogu R N. 2007. Feed intake and nutrient digestibility of West African dwarf (WAD) goat fed Pennisetum purpureum supplemented with Gmelina arborea. Animal Research International 4 (3): 724–27. DOI:

Ouda J O, NsahlaiI V and Mahundu P M.2006. In vitro degradability and gas production parameters of Sericea lespedeza (Lespedeza cuneata) mixed with varying types and levels of roughages. South African Journal of Animal Science 36 (2): 111–21. DOI:

Purcell P J, Boland T M, O’Brien M and O’Kelly P. 2012. In vitro rumen methane output of forb species sampled in spring and summer. Agricultural and Food Science 21: 83–90. DOI:

Salem A Z M, El Dawy M M and Robinson P H. 2006. Nutritive evaluation of some browses tree foliage during dry season: Secondary compounds, feed intake and in vivo digestibility in sheep and goats. Animal Feed Science and Technology 127: 251–67. DOI:

Theodorou M K, Williams B A, Dhanoa M S, Mc Allan A B and France J. 1994. A simple gas production method using pressure transducer to determine the fermentation kinetics of ruminant feeds. Animal Feed Science Technology 48: 185–97. DOI:

Tilley J M A and Terry R A. 1963. A two-stage technique for the in vitro digestion of forage crops. Journal of British Grassland Society 18: 104–11. DOI:

Tolera A. 2007. Forage: A Pathway to Prosperity for Smallholder Farmers. Proceedings of an International Forage Symposium. Ubon Ratchathani University, Thailand, pp 165–86.

Van Soest P J, Robertson J B and Lewis B A. 1991. Method for dietary fibre, Neutral detergent fibre and non-starch polysaccharides in relation to animal nutrition. Journal of Dairy Science 74: 3588–97. DOI:









How to Cite

SINGH, S., & SINGH, B. B. (2015). In vitro evaluation of Dichanthium annulatum (Marvel grass) grass hay diets supplemented with browse foliage. The Indian Journal of Animal Sciences, 85(12), 1348–1353.