Profiling of bovine toll like receptors (TLRs) in foot and mouth disease vaccinated cattle


Abstract views: 303 / PDF downloads: 100

Authors

  • RAJEEV RANJAN ICAR-Project Directorate on Foot-and-Mouth Disease, Mukteshwar, Nainital, Uttarakhand 263 138 India
  • JITENDRA KUMAR BISWAL ICAR-Project Directorate on Foot-and-Mouth Disease, Mukteshwar, Nainital, Uttarakhand 263 138 India
  • AJAY KUMAR SHARMA TAH, IVRI Campus Mukteshwar
  • JYOTI MISRI Indian Council of Agricultural Research, Krishi Bhawan, New Delhi
  • BRAMHADEV PATTNAIK ICAR-Project Directorate on Foot-and-Mouth Disease, Mukteshwar, Nainital, Uttarakhand 263 138 India

https://doi.org/10.56093/ijans.v86i4.57688

Keywords:

, Cattle, FMDV, Real-time RT-PCR, TLRs profile, Vaccine

Abstract

Foot and mouth disease virus (FMDV) elicits acute humoral antibody response in both infected and vaccinated animals. Toll like receptors (TLRs) are type 1 transmembrane proteins expressed in almost all cell types and activate the innate immune system. The current study was performed to evaluate expression profiling of bovine TLRs like TLR 2, TLR 3, TLR 7, TLR 8 and TLR 10, in response to FMD inactivated vaccine using quantitative real-time RT-PCR technique. Blood samples were collected from control, test group 1 and test group 2, at 0, 14th and 21st days post-vaccination (dpv). The mRNA abundance of these target genes was calibrated with a housekeeping gene (18 S) and expressed as fold over expression of the TLRs genes in bovine over the 0th dpv as control. On 0 day, expression of all TLRs did not vary significantly. The expression of TLR2 and TLR3 genes significantly increased in both test group 1 and 2 after 14th day and 21st DPV but expression of other TLRs increase in test groups 1 and 2 did not differ significantly. Expression of TLR2 and TLR3 genes considerably increased in test group 1 and 2 but expression of these genes were more in test group 1 as compared to test group 2. From preliminary findings, if there is inclusion of TLR2 and TLR 3 agonist in vaccine, it may enhance the innate immunity of animals and helps in clearing of virus and may prevent establishment of infection.

Downloads

Download data is not yet available.

References

Alexandersen S, Zhang Z, Donaldson A I, Garland A J. 2003. The pathogenesis and diagnosis of foot-and-mouth disease. Comparative Pathology 129: 1–36. DOI: https://doi.org/10.1016/S0021-9975(03)00041-0

Belsham G J. 1993. Distinctive features of foot-and-mouth disease virus, a member of the picornavirus family; aspects of virus protein synthesis, protein processing and structure. Progress in Biophysics and Molecular Biology 60: 241–60. DOI: https://doi.org/10.1016/0079-6107(93)90016-D

Biswal J K, Sanyal A, Rodriguez L L, Subramaniam S, Arzt J, Sharma G K, Hammond J M, Parida S, Mohapatra J K, Mathapati B S, Dash B B, Ranjan R, Rout M, Venketaramanan R, Misri J, Krishna L, Prasad G, Pathak K M L and Pattnaik B. 2012. Foot-and-mouth disease: Global status and Indian perspective. Indian Journal of Animal Sciences 82 (2): 109–31.

Cheng G, Zhao X, Yan W, Wang W, Zuo X, Huang K, Liu Y, Chen J, Wang J, Cong W, Liu M, Gao H, Lu Y and Zheng Z. 2007. Alpha interferon is a powerful adjuvant for a recombinant protein vaccine against foot- and-mouth disease virus in swine, and an effective stimulus of in vivo immune response. Vaccine 25 (28): 5199–208. doi:10.1016/j.vaccine.2007.04.089 DOI: https://doi.org/10.1016/j.vaccine.2007.04.089

Doyle S E, O’Connell R, Vaidya S A, Chow E K, Yee K and Cheng G. 2003. Toll-like receptor 3 mediates a more potent antiviral response than Toll like receptor 4. Journal of Immunology 170: 3565– 71. DOI: https://doi.org/10.4049/jimmunol.170.7.3565

Gaikwad S, Kumar S, Prashanth T, Reddy G R, Suryanarayana V S, Dechamma H. 2012. Transcriptional expression profile of toll like receptor 1–10 mRNA in bovine peripheral mononuclear cells in response to foot and mouth disease antigens. Advances in Microbiology 2: 417–25. DOI: https://doi.org/10.4236/aim.2012.24053

Gilliet M, Cao W and Liu Y J. 2008. Plasmacytoid dendritic cells: sensing nucleic acids in viral infection and autoimmune diseases. Nature Reviews Immunology 8: 594. DOI: https://doi.org/10.1038/nri2358

Hemmi H, Kaisho T, Takeuchi O, Sato S, Sanjo H, Hoshino K, Horiuchi T, Tomizawa H, Takeda K and Akira S. 2002. Small anti-viral compounds activate immune cells via the TLR 7 MyD88-dependent signaling pathway. Nature Immunology 3: 196–200. DOI: https://doi.org/10.1038/ni758

Kawai T and Akira S. 2007. TLR signaling. Semin Immunology 19: 24–32. DOI: https://doi.org/10.1016/j.smim.2006.12.004

Kawai T and Akira S. 2010. The role of pattern recognition receptors in innate immunity: update on Toll like receptors. Nature Immunology 11: 373–84. DOI: https://doi.org/10.1038/ni.1863

Knowles N J and Samuel A R. 2003. Molecular epidemiology of foot-and-mouth disease virus. Virus Research 91: 65–80. DOI: https://doi.org/10.1016/S0168-1702(02)00260-5

Lahmers K K, Hedges J F, Jutila M A, Deng M, Abrahamsen M S and Brown W C. 2006. Comparative gene expression by WC1 ϒδ and CD4 αβ T lymphocytes, which respond to Anaplasma

marginale, demonstrates higher expression of chemokines and other myeloidcell-associated genes by WC1 ϒδ T cells. Journal of Leukocyte Biology 80: 939– 52. DOI: https://doi.org/10.1189/jlb.0506353

Latz E, Schoenemeyer A, Visintin A, Fitzgerald K A, Monks B G, Knetter C F, Lien E, Nilsen N J, Espevik T and Golenbock D T. 2004. TLR9 signals after translocating from the ER to CpG DNA in the lysosome. Naure Immunololgy 5(2): 190–8. DOI: https://doi.org/10.1038/ni1028

Liang Shu-Mei and Chang Yu-Ching. 2014. Tlr-2 agonists and methods of use thereof. United States Patent. Patent No.: US 8,795,678 B2.

Menzies M and Ingham A. 2006. Identification and expression of Toll-like receptors 1–10 in selected bovine and ovine tissues. Veterinary Immunology and Immunopathology 109: 23–30. DOI: https://doi.org/10.1016/j.vetimm.2005.06.014

Mohapatra J K, Subramaniam S, Pandey L K, Pawar S S, De A, Das B, Sanyal A and Pattnaik B. 2011. Phylogenetic structure of serotype A foot-and-mouth disease virus: global diversity and the Indian perspective. Journal of General Virology 92: 873– 79. DOI: https://doi.org/10.1099/vir.0.028555-0

Nishiya T, Kajita E, Miwa S and Defranco A L. 2005. TLR3 and TLR7 are targeted to the same intracellular compartments by distinct regulatory elements. The Journal of Biological Chemistry 280: 37107. DOI: https://doi.org/10.1074/jbc.M504951200

Ranjan R, Kangayan M, Subramaniam S, Mohapatra J K, Biswal J K, Sharma G K, Sanyal A and Pattnaik B. 2014. Development and evaluation of a one step reverse transcription-loop mediated isothermal amplification assay (RT-LAMP) for rapid detection of foot and mouth disease virus in India. Virus Disease 25 (3): 358–64. DOI: https://doi.org/10.1007/s13337-014-0211-2

Robson K J, Harris T J and Brown F. 1977. An assessment by competition hybridization of the sequence homology between the RNAs of the seven serotypes of FMDV. Journal of General Virology 37: 271–76. DOI: https://doi.org/10.1099/0022-1317-37-2-271

Schmittgen T D and Livak KJ. 2008. Analyzing real-time PCR data by the comparative CT method. Nature Protocols 3: 1101– 08. DOI: https://doi.org/10.1038/nprot.2008.73

Schulz O, Diebold S S, Chen M, Näslund T I, Nolte M A, Alexopoulou L, Azuma Y T, Flavell R A, Liljeström P and Sousa C R. 2005. Toll-like receptor 3 promotes cross-priming to virus-infected cells. Nature 433: 887– 92. DOI: https://doi.org/10.1038/nature03326

Summerfield A, Guzylack-Piriou L, Harwood L and McCullough K C. 2009. Innate immune responses against foot- and-mouth disease virus: current understanding and future directions. Veterinary Immunology and Immunopathology 128 (1–3): 205–10. DOI: https://doi.org/10.1016/j.vetimm.2008.10.296

Takeda K and Akira S. 2005. Toll-like receptors in the innate immunity. International Immunology 17: 1–4. DOI: https://doi.org/10.2174/1568014053005336

Downloads

Submitted

2016-04-18

Published

2016-04-22

Issue

Section

Articles

How to Cite

RANJAN, R., BISWAL, J. K., SHARMA, A. K., MISRI, J., & PATTNAIK, B. (2016). Profiling of bovine toll like receptors (TLRs) in foot and mouth disease vaccinated cattle. The Indian Journal of Animal Sciences, 86(4), 367–371. https://doi.org/10.56093/ijans.v86i4.57688
Citation