Prognostic value of post thaw semen quality parameters, mitochondrial integrity and cholesterol content of sperm membrane vis-à-vis conception rate in Frieswal bulls

N SRIVASTAVA; MEGHA PANDE; T V RAJA; S TYAGI; SURESH KUMAR; SUSHIL KUMAR; RAVINDER KUMAR; A S SIROHI; NAIMI CHAND; S ARYA; AMIT KUMAR; OMER DIN

doi:10.56093/ijans.v88i8.82911

Authors

N SRIVASTAVA Senior Scientist, ICAR-Central Institute for Research on Cattle, Meerut, Uttar Pradesh 250 001 India
MEGHA PANDE Scientist, ICAR-Central Institute for Research on Cattle, Meerut, Uttar Pradesh 250 001 India
T V RAJA Senior Scientist, ICAR-Central Institute for Research on Cattle, Meerut, Uttar Pradesh 250 001 India
S TYAGI Principal Scientist, ICAR-Central Institute for Research on Cattle, Meerut, Uttar Pradesh 250 001 India
SURESH KUMAR Principal Scientist, ICAR-Central Institute for Research on Cattle, Meerut, Uttar Pradesh 250 001 India
SUSHIL KUMAR Principal Scientist, ICAR-Central Institute for Research on Cattle, Meerut, Uttar Pradesh 250 001 India
RAVINDER KUMAR Senior Scientist, ICAR-Central Institute for Research on Cattle, Meerut, Uttar Pradesh 250 001 India
A S SIROHI Senior Scientist, ICAR-Central Institute for Research on Cattle, Meerut, Uttar Pradesh 250 001 India
NAIMI CHAND Senior Scientist, ICAR-Central Institute for Research on Cattle, Meerut, Uttar Pradesh 250 001 India
S ARYA Senior Research Fellow, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh
AMIT KUMAR Senior Scientist, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh
OMER DIN MVSc Scholar, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh

https://doi.org/10.56093/ijans.v88i8.82911

Keywords:

Cholesterol content, Conception rate, Frieswal bulls, Mitochondrial membrane potential, Quality parameters

Abstract

The present study aimed to analyze the quantitative relationship of membrane cholesterol content (Chol content), mitochondrial integrity (Δψm), and quality parameters of spermatozoa (SQP) at post-thaw stage with conception rate (CR) and estimated relative conception rate (ERCR) in Frieswal bulls. For the experiment, frozen semen straws (32) were collected from the SF laboratory and CR (Total insemination 3482) was obtained from Field Progeny Testing units. Based on the CR, bulls were grouped into low, medium or high groups (G I, G II and G III, respectively). SQP, viz. viability (Eosin Nigrosin), post thaw motility, biochemical integrity of the membrane (HOS res), acrosome integrity (Giemsa, and fluorochromes fluorescein isothiocyanate Pisum sativum agglutinin and propidium iodide, respectively), chol-content, and Δψm using fluorescent probe JC-1 (5,5’,6,6’-tetrachloro- 1,1’,3,3’-tetraethylbenzimi-dazolylcarbocyanine iodide) were determined. The values thus obtained were subjected to the stepwise regression analysis using the least squares principles for each group, and the CR and ERCR were regressed on the various SQP. Pearson’s correlation coefficients were estimated between the CR and ERCR values and the various SQPs. The coefficient of determination (R2) moderately higher for all the models and ranged from 63.70–93.40% (high and medium group, respectively). High R2 value of the prediction equation for the herd and bulls with medium CR (75.9 and 93.4%, respectively) reveal their suitability to predict the CR and ERCR potential of the cryopreserved semen. Study results point to inclusion of cholesterol content and Δψm estimation in routine semen analysis before long-term storage or usage for insemination purposes.

Downloads

Download data is not yet available.

Author Biography

RAVINDER KUMAR, Senior Scientist, ICAR-Central Institute for Research on Cattle, Meerut, Uttar Pradesh 250 001 India

Senior Scientist,Â ICAR-Central Institute for Research on Cattle, Meerut, Uttar Pradesh 250 001 India

References

Asadpour R, Alavi-Shoushtari S, Rezaii A and Ansari A H K. 2007. SDS-polyacrylamide gel electrophoresis of buffalo bulls seminal plasma proteins and their relation with semen freezability. Animal Reproduction Science 102: 308–13. DOI: https://doi.org/10.1016/j.anireprosci.2007.03.003

Amorim E A M, Graham J K, Spizziri B, Meyers M and Torres C A A. 2009. Effect of cholesterol or cholesteryl conjugates on the cryosurvival of bull sperm. Cryobiology 58: 210–14. DOI: https://doi.org/10.1016/j.cryobiol.2008.12.007

Brouwers J F, Silva P F and Gadella B M. 2005. New assays for detection and localization of endogenous lipid peroxidation products in living boar sperm after BTS dilution or after freeze–thawing. Theriogenology 63: 458–69. DOI: https://doi.org/10.1016/j.theriogenology.2004.09.046

Cottet-Rousselle C, Ronot X, Leverve X and Mayol J. 2011. Cytometric assessment of mitochondria using fluorescent probes. Cytometry 79: 405–25. DOI: https://doi.org/10.1002/cyto.a.21061

Dogan S, Mason M C, Govindaraju A, Belser L, Kaya A, Stokes J, Rowe D and Memili E. 2013. Interrelationships between apoptosis and fertility in bull sperm. Journal of Reproduction and Development 59: 18–26. DOI: https://doi.org/10.1262/jrd.2012-068

Gillan L, Kroetsch T, Maxwell W M C and Evans G. 2008. Assessment of in vitro sperm characteristics in relation to fertility in dairy bulls. Animal Reproduction Science 103: 201–14. DOI: https://doi.org/10.1016/j.anireprosci.2006.12.010

Gliozzi T, Turri F, Manes S, Cassinelli C and Pizzi F. 2017. The combination of kinetic and flow cytometric semen parameters as a tool to predict fertility in cryopreserved bull semen. Animal 11(11): 1975–82. DOI: https://doi.org/10.1017/S1751731117000684

Gopalkrishnan K, Hinduja I N and Anandkumar T C. 1991. Assessment of mitochondrial activity of human spermatozoa with reference to motility and viability in fertile and infertile men. Molecular Andrology 3: 243–50.

Harvey W R. 1990. Users Guide for LSMLMW and MIXMDL package, PC2 version. Mimeograph, Columbus, Ohio, USA.

Hussain Ahmed S, Mutase Hassan Andrei and Sara Jahan. 2016. Semen quality parameters as fertility predictors of water buffalo bull spermatozoa during low-breeding season. Theriogenology 86: 1516–22. DOI: https://doi.org/10.1016/j.theriogenology.2016.05.010

Jeyendran R S, Vander Ven H H, Parez-Pelaez M, Crabo B G and Zaneweld L J D. 1984. Development of an assay to assess the functional integrity of the human membrane and its relationship to other semen characteristics. Journal of Reproduction and Fertility 70: 219–28. DOI: https://doi.org/10.1530/jrf.0.0700219

Kasai T, Ogawa K, Mizuno K, Nagai S, Uchida Y, Ohta S, Fujie M, Suzuki K, Hirata S and Hoshi K. 2002. Relationship between sperm mitochondrial membrane potential, sperm motility, and fertility potential. Asian Journal of Andrology 4: 97–103.

Manjunath P, Nauc V, Bergeron A and Menard M. 2002. Major proteins of bovine seminal plasma bind to the low-density lipoprotein fraction of hen’s egg yolk. Biology of Reproduction 67: 1250–58. DOI: https://doi.org/10.1095/biolreprod67.4.1250

Moce E and Graham J K. 2006. Cholesterol-loaded cyclodextrins added to raw bull ejaculates improves sperm cryosurvival. Journal of Animal Science 84: 826–33. DOI: https://doi.org/10.2527/2006.844826x

Mukhopadhyay C S, Verma A, Joshi B K, Singh Avtar, Chakravarty A K and Dubey P P. 2008. In vitro acrosome reaction: its pertinence in assessing fertility of cryopreserved semen. Indian Journal of Animal Research 42: 201–04.

Mukhopadhyay C S, Verma A, Joshi B K and Chakravarty A K. 2008. Association of sperm mitochondrial activity index with gross seminal parameters and fertility in crossbred bulls. Indian Journal of Animal Research 42: 282–84.

Odhiambo J F, DeJarnette J M, Geary T W, Kennedy C E, Suarez S S, Sutovsky M and Sutovsky P. 2014. Increased conception rates in beef cattle inseminated with nanopurified bull semen. Biology of Reproduction 91: 1–10. DOI: https://doi.org/10.1095/biolreprod.114.121897

Pesch S and Bergmann B. 2006. Structure of mammalian spermatozoa in respect to viability, fertility and cryopreservation. Micron 37: 597–612. DOI: https://doi.org/10.1016/j.micron.2006.02.006

Rodriguez-Martinez H. 2000. Evaluation of frozen-semen: traditional and new approaches, pp 502–600. Topics in Bull Fertility. 1st edn. (Ed.) Chenoweth P J. International Veterinary Information Service, Ithaca, NY.

RSparr E, Halin L, Markova N and Wennerstrom H. 2002. Phospholipids-cholesterol bilayers under osmotic stress. Biophysics Journal 83: 2015–25. DOI: https://doi.org/10.1016/S0006-3495(02)73963-5

Srivastava N, Srivastava S K, Ghosh S K, Amit Kumar, Perumal P and Jerome A. 2013. Acrosome membrane integrity and cryocapacitation are related to cholesterol content of bull spermatozoa. Asian Pacific Journal of Reproduction 2: 126–31. DOI: https://doi.org/10.1016/S2305-0500(13)60132-3

Strom H B, Larsson B, Linde-Forsberg C and Rodriguez-Martinez H. 2000. Evaluation of chilled and frozen-thawed canine spermatozoa using a zona pellucida binding assay. Journal of Reproduction and Fertility 119: 201–06. DOI: https://doi.org/10.1530/jrf.0.1190201

Sukardi S, Curry M R and Watson P F. 1997. Simultaneous detection of the acrosomal status and viability of incubated ram spermatozoa using fluorescent markers. Animal Reproduction Science 46: 89–96. DOI: https://doi.org/10.1016/S0378-4320(96)01599-0

Tartaglione C M and Ritta M N. 2004. Prognostic value of spermatological parameters as predictors of in vitro fertility of frozen-thawed bull semen. Theriogenology 62: 1245–52. DOI: https://doi.org/10.1016/j.theriogenology.2004.01.012

Thomas C A, Garner D L, DeJarnette J M and Marshall C E. 1998. Effect of cryopreservation of bovine sperm organelle function and viability as determined by flow cytometry. Biology of Reproduction 58: 786–93. DOI: https://doi.org/10.1095/biolreprod58.3.786

Vyt P, Maes D, Rijsselaere T, Dejonckheere E, Castryck F and Van Soom A. 2004. Motility assessment of porcine spermatozoa: a comparison of methods. Reproduction in Domestic Animals 39: 447–53. DOI: https://doi.org/10.1111/j.1439-0531.2004.00538.x

Watson P F. 1975. Use of Giemsa stain to detect change in acrosome of frozen ram spermatozoa. Veterinary Record 97: 12–15. DOI: https://doi.org/10.1136/vr.97.1.12

Zavos P M and Centola G M. 1990. Qualitative and quantitative improvements in human spermatozoa recovered via the swimup and a new semen filtration method. Infertility 13: 25–34.

Zhang B R, Larsson B, Lundeheim N, Haard M G and Rodriguez- Martinez H. 1999. Prediction of bull fertility by combined in vitro assessments of frozen-thawed semen from young dairy bulls entering an AI-program. International Journal of Andrology 22: 253–60. DOI: https://doi.org/10.1046/j.1365-2605.1999.00178.x