Biodegradation of the pyrethroid pesticide cyfluthrin by the halophilic bacterium Photobacterium ganghwense isolated from coral reef ecosystem


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Authors

  • V. S. Jayasree Central Marine Fisheries Research Institute - Kochi.
  • K. S. Sobhana Central Marine Fisheries Research Institute, Kochi.
  • Priyanka Poulose Central Marine Fisheries Research Institute - Kochi Cochin University of Science And Technology
  • Keerthi R. Babu Central Marine Fisheries Research Institute - Kochi Cochin University of Science And Technology
  • S. Jasmine Central Marine Fisheries Research Institute - Kochi
  • L. Ranjith Central Marine Fisheries Research Institute - Kochi
  • R. Saravanan Central Marine Fisheries Research Institute - Kochi.
  • H. Jose Kingsly Central Marine Fisheries Research Institute - Kochi.
  • K. R. Sreenath Central Marine Fisheries Research Institute - Kochi.
  • K. K. Joshi Central Marine Fisheries Research Institute - Kochi.
  • Rani Mary George Central Marine Fisheries Research Institute - Kochi.
  • A. Gopalakrishnan

https://doi.org/10.21077/ijf.2020.67.4.100553-14

Abstract

A halophilic bacterial strain T14 isolated from the mucus of coral Acropora sp. was found to be highly effective in degrading the pyrethroid pesticide, cyfluthrin. T14 was identified as Photobacterium ganghwense (GenBank Accession No. MT360254) based on phenotypic and biochemical characteristics as well as by 16S rRNA gene sequence analysis. The pyrethroid degrading efficiency of P. ganghwense T14 strain was examined under different culture conditions. It was observed that P. ganghwense T14 was able to utilise cyfluthrin as a sole carbon source and was found to grow on mineral medium with pesticide concentrations ranging from 10 to 100 mg l-1. Optimal temperature and pH conditions for efficient cyfluthrin degradation by P. ganghwense T14 were determined as 30° C and 8 respectively. Degradation of cyfluthrin by P. ganghwense T14 was quantitated by gas chromatography-tandem mass spectrometry (GC-MS/MS). Mineral medium supplemented with 100 mg l-1 cyfluthrin and inoculated with P. ganghwense T14 (106 cells ml-1) recorded 92.13% pesticide decomposition within 120 h. Cytotoxicity assay on a fish cell line EM4SpEx derived from the grouper Epinephelus malabaricus, revealed a drastic reduction in cyfluthrin toxicity as evidenced by reduction in the intensity of cell destruction as well as morphological changes when exposed to P. ganghwense T14 treated filtrate, in comparison with that of parent cyfluthrin filtrate. Results of the study clearly indicated potential bioremediative use of P. ganghwense T14 in cyfluthrin contaminated sites.

Keywords: Biodegradation, Cyfluthrin, Cytotoxicity, Halophilic bacterium, Pesticide degradation, Photobacterium ganghwense, Pyrethroid

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Submitted

2020-05-15

Published

2020-12-31

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Articles

How to Cite

Jayasree, V. S., Sobhana, K. S., Poulose, P., Babu, K. R., Jasmine, S., Ranjith, L., Saravanan, R., Kingsly, H. J., Sreenath, K. R., Joshi, K. K., George, R. M., & Gopalakrishnan, A. (2020). Biodegradation of the pyrethroid pesticide cyfluthrin by the halophilic bacterium Photobacterium ganghwense isolated from coral reef ecosystem. Indian Journal of Fisheries, 67(4). https://doi.org/10.21077/ijf.2020.67.4.100553-14