In-vitro Studies on Efficacy of Native Entomopathogenic Nematodes (Steinernema carpocapsae) on Cattle Ticks
Efficacy of Entomopathogenic Nematodes on Cattle Ticks
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Authors
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Subhash Kachhawaha
KVK, ICAR-Central Arid Zone Research Institute, Jodhpur 342 003, India
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Ajay Kumar Maru
B.A. College of Agriculture, A.A.U., Anand 388 110, India
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A.U. Siddiqui
Rajasthan Collage of Agriculture, M.P.U.A.T., Udaipur 313 004, India
Abstract
Entomopathogenic nematodes (EPNs) have been
successfully used as biological control agents for insects
of economically important crops. In the present study,
bio-efficacy of two different strains of entomopathogenic
nematodes, Steinernema carpocapsae STSLU and S. carpocapsae
STUDR against two different cattle hard ticks, Rhipicephalus
microplus and Hyalomma savignyi was evaluated based on
percentage mortality under laboratory conditions. The
adult female cattle ticks (of both species) were treated with
infective juveniles (IJs) of both the strains of S. carpocapsae at
different inoculum levels. All the treatments were replicated
four times at 20ºC. Percentage mortality of the cattle ticks
was determined every 24 hours up to 120 hours from the
time of inoculation. The experimental results showed the
cattle tick R. microplus was more susceptible to both strains
of Steinernema carpocapsae than that of H. savignyi. Further,
S. carpocapsae STSLU was more efficient than S. carpocapsae
STUDR and caused 100 and 97.5% mortality of R. microplus
and H. savignyi, respectively at a concentration of 250 IJs Petri
dish-1 after 120 hours of inoculation. The entomopathogenic
nematodes can be cultured easily in an artificial medium
and have high reproductive efficiency, broad host range,
long storage ability, ease of application and being safe for
the host make them promising bio-control agent against R.
microplus and H. savignyi. This may be evaluated further in
field conditions in different seasons and temperatures. Future
research may be directed towards emerging technologies of
ticks control without acaricide uses
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References
Akhurst, R. and Boemare, N.E. 1990. Biology and taxonomy of Xenorhabdus, In: Entomopathogenic Nematodes in Biological Control (Eds. R. Gaugler and H.K. Kaya ), pp. 75-87. Boca Raton, CRC Press.
Boemare, N.E., Akhurst, R.J. and Mourant, R.G. 1993. DNA relatedness between Xenorhabdus spp. (Enterobacteriacae) symbiotic bacteria of entomopathogenic nematodes, and a proposal to transfer Xenorhabdus luminescens to a new genus, Photorhabdus gen. novo. The International Journal of Systematic and Evolutionary Microbiology 43: 249-255.
Esteve-Gasent, M.D., Rodriguez-Vivas, R.I., Medina, R.F., Ellis, D., Schwartz, A. and Cortes Garcia, B. 2020. Research on integrated management for cattle fever ticks and bovine babesiosis in the United States and Mexico: current status and opportunities for binational coordination. Pathogens 9:871. doi: 10.3390/pathogens 9110871.
FAO (2004) Ticks: Acaricide resistance, diagnosis, management and prevention. In: Resistance Management and Integrated Parasite Control in Ruminants: Guidelines Module 1. Animal Production and Health Division, Food and Agriculture Organization of the United Nations, Rome. 25-77.
Fernanedz-salas, A., Alonoso-diaz, M.A., Alonoso-morale, R.A., Lezama-gutierrez, R., Rodriguez-rodroguez, J.C. and Cervantes-chavez, J.A. 2017. Acaricidal activity of Metarhizium manisopliae isolated from paddocks in the Mexican tropics against two populations of the cattle tick Rhipicephalus microplus. Medical and Veterinary Entomology 31: 36-43.
Georgis, R.H., Kaya, H.K. and Gaugler, R. 1991. Effect of steinernematid and heterorhabditid nematodes on non-target arthopods. Environmental Entomology 20: 815-822.
Ghosh, S., Azhahianambi, P. and Yadav, M.P. 2007. Upcoming and future strategies of tick control: a review. Journal of Vector Borne Diseases 44: 79-89.
Grisi, L., Leite, R.C., Martins, J.R.S., Barros, A.T.M., Andreotti, R., Cançado, P.D. and Villela, H.S. 2014. Reassessment of the potential economic impact of cattle parasites in Brazil. Brazilian Journal of Veterinary Parasitology 23:150-156.
Hassanain, M.A., Derbala, A.A., Abdel-barry, N.A., El-Sherif, M.A. and El-Sadawy, H.A.E. 1999. Biological control of ticks (Argasidae) by entomopathogenic nematodes. Egyptian Journal of Biological Pest Control 7: 41–46.
Kaur, D., Jaiswal, K., Mishra, S. 2016. Methods of Tick Control: Conventional and Novel Approaches. European Journal of Pharmaceutical and Medical Research 3: 110–115.
Kocan, K.M., Blouin, E.F., Pidherney, M.S., Claypool, P.L., Samish, M. and Glazer, I. 1998. Entomopathogenic nematodes as a potential biological control method for ticks. Annals of the New York Academy of Sciences June 29; 849: 355-364.
Kocan, K.M., Pidherney, M.S., Blouin, E.F., Claypool, P.L., Samish, M. and Glazer, I. 1998. Interaction of entomopathogenic nematodes (Steiner nematidae) with selected species of ixodid ticks (Acari: Ixodidae). Journal of Medical Entomology 35: 514-520.
Kumar, A.S. and Nagarajan, K. 2013. A study on prevalence status of infection among cattle population of Kancheepuram and in and around Chennai districts of Tamil Nadu. International Journal of Food, Agriculture and Veterinary Science 3: 155-157.
Maru, A.K., Kachhawaha, S., Siddiqui, A.U. and Sharma, S.K. 2011. Use of entomopathogenic nematode Steinernema carpocapsae (STSLU) for the biological control of cattle ticks Rhipicephalus microplus. Veterinary Practice 12 (2): 233-235.
Mondal, D.B., Sarma, K. and Saravanan, M. 2013. Upcoming of the integrated tick control program of ruminants with special emphasis on livestock farming system in India. Ticks and Tick-borne Diseases 4: 1-10.
Monteiro, C.M.D., Prata, M.C.D., Furlong, J., Faza, A.P., Mendes, A.S., Andalo, V. and Moino, A. 2010. Heterorhabditis amazonensis (Rhabditidae: Heterorhabditidae), strain RSC-5, for biological control of the cattle tick Rhipicephalus (Boophilus) microplus (Acari: Ixodidae). The Journal Parasitology Research 106: 821-826.
Perez de Leon, A.A., Mitchell, R.D., Mille, R.J. and Lohmeyer, K.H. 2021. Advances in integrated tick management research for area-wide mitigation of tick-borne disease burden. In Area-Wide Integrated Pest Management: Development and Field Application; Pereira, R, Vreysen, MJB, Eds. CRC Press: Boca Raton, FL, USA, pp. 251–274.
Samish, M., Alekseev, E. and Glazer, I. 1999. Efficacy of entomopathogenic nematode strains against engorged Boophilus annulatus females (Acari: Ixodidae) under simulated field conditions. Journal of Medical Entomology 36 (6): 727-732.
Samish, M., Alekseev, E. and Glazer, I. 2000. Mortality rate of adult ticks due to infection by entomopathogenic nematodes. Journal of Parasitology 86 (4): 679-84.
Samish, M., Gindin, G., Alekseev, E. and Glazer, I. 2001. Pathogenicity of entomopathogenic fungi to different developmental stages of Rhipicephalus sanguineous. The Journal of Parasitology 87: 1355-1359.
Seebeck, R.M., Springell, P.H.O. and Kelly, J.C. 1971. Alterations in the host metabolism by the specific and anorectic effects of the cattle tick (Boophilus microplus) in food intake and body weight growth. Australian Journal of Biological Sciences 24: 373-380.
Shapiro, I., Gouge, D.H. and Koppeenhfer, A.M. 2002. Factors affecting commercial success: case study in cotton, turf and citrus. In: Entomopathogenic Nematology (Ed. R. Gaugler), pp. 333-356. CABI, New York.
Zangi, G. 2003. Tick, Control by Means of Entomopathogenic Nematodes and Fungi, M.Sc. Thesis, The Hebrew University of Jerusalem, Israel.
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