Exploring the Acaricidal Potential of Calotropis procera and Lantana camara Extracts Against Rhipicephalus microplus Ticks

Aniruddha Singh  Niranjan; Jitendra Singh  Tiwari; Mukesh Kumar  Srivastava; Sonika Verma; Daya Shanks

doi:10.56093/aaz.v64i2.166301

Authors

Anuruddha Singh Niranjan Uttar Pradesh Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go-Anusandhan Sansthan, Mathura 281001,
Jitendra Singh Tiwari Uttar Pradesh Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go-Anusandhan Sansthan, Mathura 281001,
Mukesh Kumar Srivastava Uttar Pradesh Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go-Anusandhan Sansthan, Mathura 281001,
Sonika Verma Uttar Pradesh Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go-Anusandhan Sansthan, Mathura 281001,
Daya Shanks Uttar Pradesh Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go-Anusandhan Sansthan, Mathura 281001,

https://doi.org/10.56093/aaz.v64i2.166301

Keywords:

Rhipicephalus microplus, Calotropis procera, Acaricide, Lantana camara, Larval Packet Test, Adult Immersion Test

Abstract

Plant-based alternative control measures for tick management are gaining significant importance due to the increasing resistance of ticks to conventional chemical acaricides and the environmental concerns associated with synthetic pesticides. Natural plant extracts, rich in bioactive compounds such as alkaloids, flavonoids, and terpenoids, offer a sustainable and eco-friendly solution for tick control. This study investigates the acaricidal properties of aqueous and ethanolic extracts of Calotropis procera and Lantana camara against Rhipicephalus microplus ticks. The efficacy of these herbal extracts was evaluated using the Adult Immersion Test (AIT) and Larval Packet Test (LPT). The highest inhibition of oviposition (IO%) was observed for the ethanolic extract of C.procera at 100 mg mL-1 concentration (100%), while the lowest was recorded for the aqueous extract of L. camara at 6.25 mg mL-1 (17.33%). Maximum larval mortality (75.99%) was
observed for the ethanolic extract of C. procera at 100 mg mL-1, and the lowest mortality (14.11%) was seen for the aqueous extract of L. camara at 6.25 mg mL-1. The LC50 (mg mL-1) value of aqueous and ethanolic extracts of L. camara and C. procera in AIT were recorded as 78.46, 58.82, 31.57 and 28.00, respectively. Likewise, the LC50 (mg mL-1) value of aqueous and ethanolic extracts of L. camara and C. procera in LPT were recorded as 406.27, 81.02, 106.88 and 32.26, respectively. These findings indicate that C. procera has significant potential as an eco- friendly alternative in integrated pest management strategies, offering a promising approach to reduce the economic burden of ticks and tick-borne diseases while promoting safer and more sustainable agricultural practices

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References

Abdel-Hady, N.M., Abdel-Halim, A.S. and Al-Ghadban, A.M., 2005. Chemical composition and insecticidal activity of the volatile oils of leaves and flowers of Lantana camara L. cultivated in Egypt. Journal of the Egyptian Society of Parasitology, 35(2), pp.687-698.

Adehan, S.B., Biguezoton, A., Adakal, H., Dossa, F., Dougnon, T.J., Youssao, E., Sessou, P., Aboh, A.B., Youssao, A.K.I., Assogba, N. and Mensah, G.A., 2016. Acaricidal activity of ethanolic and volatile extracts of the leaves of selected plants used in veterinary pharmacopeia on the larvae of Rhipicephalus microplus in Benin. Alexandria Journal of Veterinary Sciences, 49(1), pp.1-11.

Al‐Rajhy, D.H., Alahmed, A.M., Hussein, H.I. and Kheir, S.M., 2003. Acaricidal effects of cardiac glycosides, azadirachtin and neem oil against the camel tick, Hyalomma dromedarii (Acari: Ixodidae). Pest Management Science: formerly Pesticide Science, 59(11), pp.1250-1254.

Baxter, G.D. and Barker, S.C., 1999. Isolation of a cDNA for an octopamine-like, G-protein coupled receptor from the cattle tick, Boophilus microplus. Insect Biochemistry and Molecular Biology, 29(5), pp.461-467.

Chen, A.C., He, H. and Davey, R.B., 2007. Mutations in a putative octopamine receptor gene in amitraz-resistant cattle ticks. Veterinary Parasitology, 148(3-4), pp.379-383.

Chungsamarnyart, N. and Jansawan, W., 1996. Acaricidal activity of peel oil of Citrus spp. on Boophilus microplus. Agriculture and Natural Resources, 30(1), pp.112-117.

Dua, V.K., Gupta, N.C., Pandey, A.C. and Sharma, V.P., 1996. Repellency of Lantana camara (Verbenaceae) flowers against Aedes mosquitoes. Journal of the American Mosquito Control Association, 12(3), pp.406-408.

Elimam, A.M., Elmalik, K.H. and Ali, F.S., 2009. Efficacy of leaves extract of Calotropis procera Ait. (Asclepiadaceae) in controlling Anopheles arabiensis and Culex quinquefasciatus mosquitoes. Saudi Journal of Biological Sciences, 16(2), pp.95-100.

FAO, 1984. Acaricide resistance: Test kit Instructions for use. World Acaricide Resistance Reference Centre, WARCC, Berlin.

Furman, D.P. and Loomis, E.C., 1984. The Ticks of California (Acari: Ixodida). University of California Publications, Bulletin of the California Insect Survey, Vol. 25. University of California Press, California, pp.1-239.

Ghisalberti, E.L., 2000. Lantana camara L. (Verbenaceae). Fitoterapia, 71(5), pp.467-486.

Ghosh, S., Tiwari, S.S., Srivastava, S., Sharma, A.K., Kumar, S., Ray, D.D. and Rawat, A.K.S., 2013. Acaricidal properties of Ricinus communis leaf extracts against organophosphate and pyrethroids resistant Rhipicephalus (Boophilus) microplus. Veterinary Parasitology, 192(1-3), pp.259-267.

Goncalves, K., Toigo, E., Ascoli, B., von Poser, G. and Ribeiro, V.S., 2007. Effects of solvents and surfactant agents on the female and larvae of cattle tick Boophilus microplus. Parasitology Research, 100, pp.1267-1270.

Hassan, M.Z., Khan, S.A., Hussain, M.A. and Ashraf, Z., 2011. Pharmacological properties of Lantana camara: a review. Journal of Ethnopharmacology, 137(1), pp.127-145.

Jain, S.C., Sharma, R., Kain, R. and Sharma, R.A., 1996. Antimicrobial activity of Calotropis procera. Fitoterapia, 67, pp.275–277.

Kumar, S., Paul, S., Sharma, A.K., Kumar, R., Tewari, S.S., Chaudhuri, P., Ray, D.D., Rawat, A.K.S. and Ghosh, S., 2011. Diazinon resistant status in Rhipicephalus (Boophilus) microplus collected from different agro-climatic regions of India. Veterinary Parasitology, 181(2-4), pp.274-281.

Mebrahtu, T., Mekonnen, S. and Asmare, K., 2019. Acaricidal activity of Calotropis procera against Rhipicephalus microplus. Tropical Animal Health and Production, 51(5), pp.1057-1063.

Singh, K., Rath, S.S. and Ghosh, S., 2020. Impact of ticks and tick-borne diseases on the livestock sector in India: an overview. Asian Journal of Veterinary Research, 10(1), pp.1-9.

Sudha, G., Sangeetha, P. and Indhu, M., 2018. Evaluation of antimicrobial and acaricidal activities of Calotropis procera leaf extracts. Asian Pacific Journal of Tropical Biomedicine, 8(1), pp.15-20.

Zaman, M.A., Iqbal, Z., Abbas, R.Z., Khan, M.N., Muhammad, G., Younus, M. and Ahmed, S., 2012. In vitro and in vivo acaricidal activity of a herbal extract. Veterinary Parasitology, 186(3-4), pp.431-436.