Effect of Herbal Feed Additives and Sulfate Supplementation on Hematology, Biochemical, and Antioxidant Status of Cattle Calves


Keywords:
Antioxidant, Calves, Herbal feed additives, Immunity, SulfateAbstract
The present study was undertaken to evaluate the effect of herbal feed additives and sulfate supplementation on the hematology, biochemical, antioxidant status and immune response of growing cattle calves. Twenty-four growing cattle calves (12 male Haryana, 8 male Sahiwal, 4 female Sahiwal calves) of 4 to 16 months of age were distributed into four groups with six animals each in a randomized block design. Control (C) group was fed on basal diet without any feed additive, T1 group was fed on basal diet with herbal feed additive (Fennel + Clove oleoresin; CLO; @ 1.0% of DMI), T2 group was supplemented with sulfate @ 0.075% of DMI, and group T3 was fed on basal diet with herbal feed additive (Fennel + CLO; @ 1.0% of DMI) and sulfate @ 0.075% of DMI. The basal diet consists of 40% concentrate and 60% wheat straw daily. Body weight and dry matter intake were recorded fortnightly. Overall body weight, DMI (kg/day), TDN intake (g/kg W0.75), and DCP intake (g/kg W0.75) were similar in the treatment as well as control group. Hematological parameters like blood hemoglobin concentration and PCV percentage were not impacted by herbal feed additive and sulfate supplementation. Plasma glucose, triacylglycerol, and cholesterol concentration were found non-significant, plasma total protein, Plasma albumin, plasma globulin and PUN concentration were also not affected (P>0.05). Alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), plasma bilirubin, plasma creatinine were found normal in range and not significantly different (P>0.05) between the groups. Total immunoglobulin level and antioxidant activity remain similar in all the groups (P>0.05). It may be concluded that supplementation of herbal feed additive (Fennel + CLO; @ 1.0% of DMI) may be used as rumen modifiers without any adverse effect in the cattle calves.
References
Aminzare, M., Hashemi, M., Ansarian, E., Bimakr, M., Hassanzad, A.H., Mehrasbi, M.R., Daneshamooz, S., Raeisi, M., Jannat, B. and Afshari, A. 2019. Using natural antioxidants in meat and meat products as preservatives: A review. Advances in Animal and Veterinary Sciences. 7(5): 417-426.
Anantasook, N., Wanapat, M. and Cherdthong, A. 2014. Manipulation of ruminal fermentation and methane production by supplementation of rain tree pod meal containing tannins and saponins in growing dairy steers. Journal of Animal Physiology and Animal Nutrition. 98(1): 50-55.
Benzie, I.F.F. and Strain, J.J. 1999. Ferric reducing antioxidant power assay: Direct measure of total antioxidant activity of biological fluids and modified version for simultaneous measurement of total antioxidant power and ascorbic acid concentration. Methods in Enzymology. 299: 15-27.
Bombik, T., Bombik, E., Frankowska, A., Trawińska, B. and Saba, L., 2012. Effect of herbal extracts on some haematological parameters of calves during rearing. Journal of Veterinary Research. 56(4): 655-658.
Cunha, C.S., Marcondes, M.I., Veloso, C.M., Mantovani, H.C., Pereira, L.G.R., Tomich, T.R., Dill‐McFarland, K.A. and Suen, G. 2019. Compositional and structural dynamics of the ruminal microbiota in dairy heifers and its relationship to methane production. Journal of the Science of Food and Agriculture. 99(1): 210-218.
Dey, A., Paul, S. S., Dahiya, S S. and Punia, B. S. 2016. Garlic oil supplementation: effects on in vitro methanogenesis, House rumen fermentation and gas production in buffaloes, In: Sreekumar, D., Jacob, N., Mahender, M., Rajanna, N. (Eds.), International Livestock Conference and Expo (INDIGENOUS) and 23rd Annual Convention Indian Society of Animal Production and Management (ISAPM) Prof. Jayashankar Telangana State Agricultural University, Hyderabad, Telangana, 41.
Johnson, D.E., Hill, T.M., Ward, G.M., Johnson, K.A., Branine, M.E., Carmean, B.R. and Lodman, D.W. 1993. Principle factors varying methane emissions from ruminants and other animals. In: Khalil, M.A.K. (Ed.), Atmospheric Methane: Sources, Sinks, and Role in Global Change. NATO AD1 Series, Springer-Verlag, Berlin, Germany, 113.
Johnson, K.A. and Johnson, D.E. 1995. Methane emissions from cattle. Journal of Animal Science, 73(8): 2483-2492.
Kumar, K. 2017. Effects of feed additives rich in essential oils on rumen fermentation, methanogenesis and nutrient utilization in buffalo, ICAR- Central Institute for Research on Buffaloes, M.V.Sc. thesis submitted to Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, India.
Kumar, K., Dey, A., Rose, M.K. and Dahiya, S.S. 2022. Impact of dietary phytogenic composite feed additives on immune response, antioxidant status, methane production, growth performance and nutrient utilization of buffalo (Bubalus bubalis) calves. Antioxidants, 11(2): 325.
Lakhani, N., Kamra, D.N., Lakhani, P. and Alhussien, M.N. 2019. Immune status and haemato-biochemical profile of buffalo calves supplemented with phytogenic feed additives rich in tannins, saponins and essential oils. Tropical animal health and production. 51(3): 565-73.
McEwan, A.D., Fisher, E.W., Selman, I.E. and Penhale W.J., 1970. A turbidity test for the estimation of immune globulin levels in neonatal calf serum. Clinica Chimica Acta, 27(1): 155-163.
Oremland, R. S. and Taylor, B. F. 1978. Sulfate reduction and methanogenesis in marine sediments. Geochim. Cosmochim. Acta. 42: 209-214.
Passetti, L. C., Passetti, R. A. and McAllister, T. A. 2021. Effect of essential oil blends and a nonionic surfactant on rumen fermentation, anti-oxidative status, and growth performance of lambs. Translational Animal Science 5(3):118.
SPSS. 2010. Statistical packages for Social Sciences, Version 20, SPSS Inc., Illinois, USA.
Yadav, P., Choudhary, S., Arora, N., Yadav, M.K., Kumawat, A. and Choudhary, S.D. 2017. Effect of feeding minerals supplementation on growth performance and nutrient utilization in Gir calves: A comparative research study. Ruminant Science, 6(2): 341-344.
Downloads
Submitted
Published
Issue
Section
License
Copyright remains with the society and author jointly. However, material can be used for research, teaching and to achieve goals of the society.