Improving Cold Tolerance in Labeo rohita: Effects of Job's Tears (Coix lacryma-jobi L.) as a Natural Feed Additive
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Authors
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Chandan Debnath
ICAR Research Complex for NEH Region
Keywords:
Aquaculture stress mitigation, Coix lacryma-jobi, Cold tolerance, Feed additive, Indian major carpAbstract
This study investigated the effects of Job's tears (Coix lacryma-jobi L.) as a feed additive on cold tolerance in Labeo rohita (initial weight: 15.2 ± 0.3 g), marking the first evaluation of its health benefits in fish or aquaculture. Three doses (low: 0.5%, medium: 1%, and high: 2% of diet) were tested over an 8-week period, with fish fed to apparent satiation twice daily. Fish were subjected to a cold stress test (150C), and physiological, biochemical, and molecular parameters were assessed. Blood samples were collected from the caudal vein of anesthetized fish at 0, 12, and 24 hours during the cold stress test. The medium dose (1%) showed the most significant improvements in cold tolerance, evidenced by enhanced survival rates, reduced plasma cortisol levels, and upregulation of cold-tolerant genes. These novel findings suggest that Job's tears could be an effective feed additive for improving cold tolerance in L. rohita aquaculture, opening new avenues for natural stress mitigation in fish farming in subtropical and temperate regions.
References
Devaraj, R., Jeepipalli, S. and Xu, B. 2020. Phytochemistry and health promoting effects of Coix lacryma-jobi - A critical review. Food Bioscience. 34: 100537.
Dong, Y., Zhang, H., Mei, J. and Xie, J. 2023. Effect of different stunning methods on antioxidant status, myofibrillar protein oxidation, and gelation properties of large yellow croaker during postmortem. Food Chemistry: X. 18: 100709.
FAO. 2022. The state of world fisheries and aquaculture 2022: Towards blue transformation. FAO.
Gond, H., Aslam, S., Ks, S., Acharya, S., Kumar, S. and Mishra, S. 2024. Job's tears (Coix lacryma-jobi): a medicinal plant of India. Zenodo. 10.5281/zenodo.10811091.
Herrera, M., Mancera, J. M. and Costas, B. 2019. The Use of Dietary additives in fish stress mitigation: comparative endocrine and physiological responses. Frontiers in Endocrinology. 10: 447.
Hsia, S. M., Yeh, C. L., Kuo, Y. H., Wang, P. S. and Chiang, W. 2007. Effects of adlay (Coix lachryma-jobi L. var. ma-yuen Stapf.) hull extracts on the secretion of progesterone and estradiol in vivo and in vitro. Experimental Biology and Medicine. 232: 1181-1194.
Iqbal, T., Elahi, A., Wijns, W. and Shahzad, A. 2023. Cortisol detection methods for stress monitoring in connected health. Health Sciences Review. 6: 100079.
Kausar, R. and Salim, M. 2006. Effect of water temperature on the growth performance and feed conversion ratio of Labeo rohita. Pakistan Veterinary Journal. 26: 105-108.
Kuebutornye, F., Roy, K., Folorunso, E. A. and Mraz, J. 2023. Plant-based feed additives in Cyprinus carpio aquaculture. Reviews in Aquaculture. 16.
Laxmisha, K. M., Semwal, D. P., Gupta, V., Katral, A., Bisht, I. S., Mehta, P. S., Arya, M., Bhardwaj, R. and Bhatt, K. C. 2022. Nutritional profiling and GIS-based grid mapping of Job's tears (Coix lacryma-jobi L.) germplasm. Applied Food Research. 2: 100169.
Manosroi, A., Sainakham, M., Chankhampan, C., Manosroi, W. and Manosroi, J. 2016. In vitro anti-cancer activities of Job's tears (Coix lachryma-jobi Linn.) extracts on human colon adenocarcinoma. Saudi Journal of Biological Sciences. 23: 248-256.
Mohammady, E. Y., Soaudy, M. R., Elashry Mohamed, A., EL-Erian, M. M. A., Farag, A., Badr, A. M. M., Bassuony, N. I., Ragaza, J. A., El-Haroun, E. R. and Hassaan, M. S. 2022. Can dietary phytogenic mixture improve performance for growth, digestive enzyme activity, blood parameters, and antioxidant and related gene expressions of Nile tilapia, Oreochromis niloticus? Animal Feed Science and Technology. 290: 115369.
Mridul, M. M. I., Zeehad, M. S. K., Aziz, D., Salin, K. R., Hurwood, D. A. and Rahi, M. L. 2024. Temperature induced biological alterations in the major carp, Rohu (Labeo rohita): Assessing potential effects of climate change on aquaculture production. Aquaculture Reports. 35: 101954.
Sánchez-Velázquez, J., Peña-Herrejón, G. A. and Aguirre-Becerra, H. 2024. Fish responses to alternative feeding ingredients under abiotic chronic stress. Animals. 14: 765.
Wang, J., Deng, L., Chen, M., Che, Y., Li, L., Zhu, L., Chen, G. and Feng, T. 2024. Phytogenic feed additives as natural antibiotic alternatives in animal health and production: A review of the literature of the last decade. Animal Nutrition. 17: 244-264.
Xu, H., Ferosekhan, S., Turkmen, S., Afonso, J. M., Zamorano, M. J. and Izquierdo, M. 2021. High broodstock fads2 expression combined with nutritional programming through broodstock diet improves the use of low fishmeal and low fish oil diets in gilthead seabream (Sparus aurata) progeny. Aquaculture. 535: 736321.
Zhang, X., Sun, Z., Wang, Y., Cao, Y., Wang, G. and Cao, F. 2022. Enhancement of growth, antioxidative status, nonspecific immunity, and disease resistance in gibel carp (Carassius auratus) in response to dietary Flos populi extract. Fish Physiology and Biochemistry. 48: 67-83.
Zheng, J. L., Luo, Z., Zhuo, M. Q. 2014. Dietary l-carnitine supplementation increases lipid deposition in the liver and muscle of yellow catfish (Pelteobagrus fulvidraco) through changes in lipid metabolism. British Journal of Nutrition. 112: 698-708.
Zhong, P., Peng, J., Bian, Z. and Huang, H. 2021. The role of cold inducible RNA-binding protein in cardiac physiology and diseases. Frontiers in Pharmacology. 12: 610792.
Zhou, S. M., Tao, Z., Shen, C., Qian, D., Wang, C. L., Zhou, Q. C. and Jin, S. 2018. β-actin gene expression is variable among individuals and not suitable for normalizing mRNA levels in Portunus trituberculatus. Fish & Shellfish Immunology. 81: 338-342.
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