Screening for okra enation leaf curl disease resistance in wild okra (Abelmoschus moschatus ssp. moschatus) germplasm of India


Abstract views: 223 / PDF downloads: 75

Authors

  • POOJA KUMARI ICAR-National Bureau of Plant Genetic Resources, New Delhi 110 012, India
  • S P SINGH ICAR-National Bureau of Plant Genetic Resources, New Delhi 110 012, India
  • K K GANGOPADHYAY ICAR-National Bureau of Plant Genetic Resources, New Delhi 110 012, India
  • V C CHALAM ICAR-National Bureau of Plant Genetic Resources, New Delhi 110 012, India
  • S C DUBEY ICAR-National Bureau of Plant Genetic Resources, New Delhi 110 012, India
  • PRAGYA RANJAN ICAR-National Bureau of Plant Genetic Resources, New Delhi 110 012, India

https://doi.org/10.56093/ijas.v91i10.117513

Keywords:

Abelmoschus moschatus ssp. moschatus, DNA-β, Field screening, OELCuV, OELCuD, Whitefly

Abstract

Okra enation leaf curl disease (OELCuD) caused by Okra enation leaf curl virus (OELCuV) is a whitefly (Bemisia tabaci) transmitted viral disease of okra which deteriorates vegetable quality and reduces yield. The OELCuV was confirmed based on the amplicon of 1.3 Kb of beta satellite (DNA-β) molecule of the virus. Field screening of wild okra (Abelmoschus moschatus ssp. moschatus) accessions was carried out for OELCuD resistance during kharif 2017-19 at experimental Farm of ICAR-National Bureau of Plant Genetic Resources, Pusa, New Delhi. Out of 76 wild okra accessions tested, 10 accessions, viz. EC360586, EC360794, EC360830, EC360900, EC359730, EC359836, EC359870, EC360351, EC361111 and EC361171 showed resistant (R) reaction in kharif 2017, whereas in kharif 2019, only four accessions, viz. EC360794, EC360586, EC360830 and EC361171 exhibited R reaction and remaining six accessions were moderately resistant (MR). In the first year, average percent disease index (PDI) was 14.15 and overall PDI ranged from 3.70 to 52.86. The range of PDI was 4.53-56.40 during the second year with an average PDI value 18.04. Apart from PDI determination, whitefly population was also monitored in both the years mainly showed moderate preference. The prominent four accessions of okra, viz. EC360794, EC360586, EC360830 and EC361171 could be utilized in resistance breeding programmes against OELCuV.

Downloads

Download data is not yet available.

References

Briddon R W, Bull S E, Mansoor S, Amin I and Markham P G. 2002. Universal primers for the PCR-mediated amplification of DNA β. Molecular Biotechnology 20(3): 315–18.

Cao B, Jian-jun L, Yong W and Guo-ju C. 2009. Inheritance and identification of SCAR marker linked to bacterial wilt-resistance in eggplant. African Journal of Biotechnology 8(20): 5201–07. 78

Doyle J J and Doyle J L. 1990. Isolation of plant DNA from fresh tissue. Focus 12(13): 39–40.

Lazarowitz S G and Shepherd R J. 1992. Geminiviruses: genome structure and gene function. Critical Reviews in Plant Sciences 11(4): 327–49.

Manoharan V, Gopalan M, Ramalkrishnan C, Rangasami P and Shanmugavelu K G. 1982. Evaluation of preference of thrips (Scirtothrips dorsalis H.) on chilli accessions. South Indian Horticulture 30(2): 155.

Naveed A, Khan A A and Khan I A. 2009. Generation mean analysis of water stress tolerance in okra (Abelmoschus esculentus L.). Pakistan Journal of Botany 41(1): 195–205.

Pasupathi, E, Murugan M, Harish S and Chinnaiah C. 2019. Screening of okra germplasm for resistance to whitefly, Bemisia tabaci and okra enation leaf curl virus (OELCV) under field conditions. Journal of Pharmacognosy and Phytochemistry 8(5): 2306–13.

Sanwal S K, Singh M, Singh B and Naik P S. 2014. Resistance to yellow vein mosaic virus and okra enation leaf curl virus: challenges and future strategies. Current Science 106(11): 1470.

Seal S E, Jeger M J and Van den Bosch F. 2006. Begomovirus evolution and disease management. Advances in Virus Research 67: 297–316.

Singh S J. 1996. Assessment of losses in okra due to enation leaf curl virus. Indian Journal of Virology 12(1): 51–53.

Singh B, Rai M, Kalloo G, Satpathy S and Pandey K K. 2007. Wild taxa of okra (Abelmoschus species): reservoir of genes for resistance to biotic stresses. Acta Horticulturae 752: 323–28. doi: 10.17660/ActaHortic.2007.752.55

Singh B, Sanwal S K, Rai M and Rai A B. 2009. Sources of biotic stress resistance in vegetable crops: A review. Vegetable Science 36(2): 133–46.

Singh B, Singh P M, Sanwal S K and Pal A K. 2014. Standardization of cost effective hybridization technique for hybrid seed production in okra (Abelmoschus esculentus). Indian Journal of Agricultural Sciences 84(9): 1111–14.

Downloads

Submitted

2021-11-02

Published

2021-11-02

Issue

Section

Articles

How to Cite

KUMARI, P., SINGH, S. P., GANGOPADHYAY, K. K., CHALAM, V. C., DUBEY, S. C., & RANJAN, P. (2021). Screening for okra enation leaf curl disease resistance in wild okra (Abelmoschus moschatus ssp. moschatus) germplasm of India. The Indian Journal of Agricultural Sciences, 91(10), 1487–1494. https://doi.org/10.56093/ijas.v91i10.117513
Citation