Effect of stearic acid on in-vitro formation of sheep oocytes

MOHAMED FARMAN; S NANDI; V GIRISH KUMAR; SHIV KUMAR TRIPATHI; P S P GUPTA

doi:10.56093/ijans.v85i6.49291

Authors

MOHAMED FARMAN MVSc Scholar, KVAFSU
S NANDI Principal Scientist, National Institute of Animal Nutrition and Physiology (NIANP), Bengaluru 560 030 India
V GIRISH KUMAR Professor and Head, Department of Biochemistry, KVAFSU
SHIV KUMAR TRIPATHI JRF, National Institute of Animal Nutrition and Physiology (NIANP), Bengaluru 560 030 India
P S P GUPTA Principal Scientist, National Institute of Animal Nutrition and Physiology (NIANP), Bengaluru 560 030 India

https://doi.org/10.56093/ijans.v85i6.49291

Keywords:

IVF, Metabolic stressors, NEFA, Stearic acid

Abstract

The present study was undertaken to study the effect of stearic acid (SA), a non esterified fatty acid (NEFA) on oocyte development of ewes (Ovis aries). Sheep oocytes were matured in vitro in the presence of different concentration of stearic acid (0 μM, 10 μM, 20 μM, and 30 μM) for 24h. After in vitro maturation, oocytes were in vitro inseminated with cauda epidydimus sperm processed with BO medium with concentration of 2 million sperms/ml and cultured on oviductal cell culture for 8 days. Oocytes were evaluated for cleavage and fertilization rates and after 7–8 days post insemination zygotes were evaluated for moruale /blastocyst stages. The maturation, cleavage and morulae/ blastocyst production rates were significantly lowered in media containing 20 μM stearic acid. Increment of stearic acid to 30 μM in media further reduced the maturation, cleavage and morulae/ blastocyst production. In conclusion, the metabolic stressors NEFA (SA) impaired the maturation, viability, cleavage and embryo production rates at the level 20 μM in ewes.

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References

Aardema H, Vos P L, Lolicato F, Roelen B A, Knijn H M, Vaandrager A B, Helms J B and Gadella B M. 2011. Oleic Acid Prevents Detrimental Effects of Saturated Fatty Acids on Bovine Oocyte Developmental Competence. Biology of Reproduction 85 (1): 62–69. DOI: https://doi.org/10.1095/biolreprod.110.088815

Brantmeier S A, Grummer R R and Ax R L. 1987. Concentrations of high density lipoproteins vary among follicular sizes in the bovine. Journal of Dairy Science 70 (10): 2145–49. DOI: https://doi.org/10.3168/jds.S0022-0302(87)80266-7

Chung B H, Tallis G A, Cho B H, Segrest J P and Henkin Y. 1995. Lipolysis-induced partitioning of free fatty acids to lipoproteins: effect on the biological properties of free fatty acids. Journal of Lipid Research 36 (9): 1956–70. DOI: https://doi.org/10.1016/S0022-2275(20)41114-9

Comin A, Gerin D, Cappa A, Marchi V, Renaville R, Motta M, Fazzini U and Prandi A. 2002. The effect of an acute energy deficit on the hormone profile of dominant follicles in dairy cows. Theriogenology 58 (5): 899–910. DOI: https://doi.org/10.1016/S0093-691X(02)00922-6

Desmet K, Van Hoeck V, Merckx E, Sirard M A, Bols P E J and Leroy J L M R. The effect of non-esterified fatty acids during in vitro culture on dnmethylation of bovine blastocysts. 30th Scientific Meeting of the European Embryo Transfer Association September 12th - 13th 2014, Dresden, Germany

Gupta P S P, Nandi S, Ravindranatha B M and Sarma P V. 2002. Trypan bluestaining to differentiate live and dead buffalo oocytes and its effect on embryo development in vitro. Buffalo Journal 18: 321–29.

Homa S T and Brown C A. 1992. Changes in linoleic acid during follicular development and inhibition of spontaneous breakdown of germinal vesicles in cumulus-free bovine oocytes. Journal of Reproduction and Fertility 94 (1): 153– 60. DOI: https://doi.org/10.1530/jrf.0.0940153

Jorritsma R, Cesar M L, Hermans J T, Kruitwagen C L, Vos P L and Kruip T A. 2004. Effects of non-esterified fatty acids on bovine granulosa cells and developmental potential of oocytes in vitro. Animal Reproduction Science 81 (3–4): 225–35. DOI: https://doi.org/10.1016/j.anireprosci.2003.10.005

Jorritsma R, de Groot M W, Vos P L, Kruip T A, Wensing T and Noordhuizen J P. 2003. Acute fasting in heifers as a model for assessing the relationship between plasma and follicular fluid NEFA concentrations. Theriogenology 60 (1): 151–61. DOI: https://doi.org/10.1016/S0093-691X(02)01358-4

Kim J Y, Kinoshita M, Ohnishi M and Fukui Y. 2001. Lipid and fatty acid analysis of fresh and frozen-thawed immature and in vitro matured bovine oocytes. Reproduction 122 (1): 131– 38. DOI: https://doi.org/10.1530/rep.0.1220131

Leroy J L, Van Soom A, Opsomer G and Bols P E. 2008. The consequences of metabolic changes in high-yielding dairy cows on oocyte and embryo quality. Animal 2 (8): 1120–27. DOI: https://doi.org/10.1017/S1751731108002383

Leroy J L, Vanholder T, Delanghe J R, Opsomer G, Van Soom A, Bols P E, Dewulf J and de Kruif A. 2004. Metabolic changes in follicular fluid of the dominant follicle in high-yielding dairy cows early post partum. Theriogenology 62 (6): 1131–43. DOI: https://doi.org/10.1016/j.theriogenology.2003.12.017

Leroy J L, Vanholder T, Mateusen B, Christophe A, Opsomer G, de Kruif A, Genicot G and Van Soom A. 2005. Non-esterified fatty acids in follicular fluid of dairy cows and their effect on developmental capacity of bovine oocytes in vitro. Reproduction 130 (4): 485–95. DOI: https://doi.org/10.1530/rep.1.00735

Lu Z H, Mu Y M, Wang B A, Li X L, Lu J M, Li J Y, Pan C Y, Yanase T and Nawata H. 2003. Saturated free fatty acids, palmitic acid and stearic acid, induce apoptosis by stimulation of ceramide generation in rat testicular Leydig cell. Biochemical Biophysical Research Communication 303 (4): 1002–07. DOI: https://doi.org/10.1016/S0006-291X(03)00449-2

Nandi S, Ravindranatha B M, Gupta P S and Sarma P V. 2002. Timing of sequential changes in cumulus cells and first polar body extrusion during in vitro maturation of buffalo oocytes. Theriogenology 57 (3): 1151–59. DOI: https://doi.org/10.1016/S0093-691X(01)00709-9

Nandi S, Pavana Shree U S and Girish V K. 2013. Metabolic stressors in ovine and caprine sera and ovarian follicular fluid. Applied Cell Biology 2: 110–13.

Roth Z, Arav A, Bor A, Zeron Y, Braw-Tal R and Wolfenson D. 2001. Improvement of quality of oocytes collected in the autumn by enhanced removal of impaired follicles from previously heat-stressed cows. Reproduction 122 (5): 737– 44. DOI: https://doi.org/10.1530/rep.0.1220737

Tanghe S, Van Soom A, Nauwynck H, Coryn M and de Kruif A. 2002. Minireview: Functions of the cumulus oophorus during oocyte maturation, ovulation, and fertilization. Molecular Reproduction and Development 61 (3): 414–24. DOI: https://doi.org/10.1002/mrd.10102

Ulloth J E, Casiano C A and De Leon M. 2003. Palmitic and stearic fatty acids induce caspase-dependent and -independent cell death in nerve growth factor differentiated PC12 cells. Journal of Neurochemistry 84 (4): 655–68. DOI: https://doi.org/10.1046/j.1471-4159.2003.01571.x

Van Hoeck V, Sturmey R G, Bermejo-Alvarez P, Rizos D, Gutierrez-Adan A, Leese H J, Bols P E J and Leroy J L M R. 2011. Elevated non-esterified fatty acid concentrations during bovine oocyte maturation compromise early embryo physiology. PLoS ONE 6(8): e23183. doi:10.1371/ journal.pone.0023183. DOI: https://doi.org/10.1371/journal.pone.0023183

Van Hoeck V, Rizos D, Gutierrez-Adan A, Pintelon J, Jorssen E, I. Dufort C , Sirard M A, Verlaet A, Hermans N, Bols P E J and Leroy J L M R. 2013. Interaction between differential gene expression profile and phenotype in bovine blastocysts originating from oocytes exposed to elevated non-esterified fatty acid concentrations. Reproduction, Fertility and Development. http://dx.doi.org/10.1071/RD13263 DOI: https://doi.org/10.1071/RD13263

van Knegsel A T M, van den Brand H, Dijkstra J and Kemp B. 2007. Effects of dietary energy source on energy balance, metabolites and reproduction variables in dairy cows in early lactation. Theriogenology 68: S274–S80. DOI: https://doi.org/10.1016/j.theriogenology.2007.04.043

Vanholder T, Leroy J L, Soom A V, Opsomer G, Maes D, Coryn M and de Kruif A. 2005. Effect of non-esterified fatty acids on bovine granulosa cell steroidogenesis and proliferation in vitro. Animal Reproduction Science 87 (1–2): 33–44. DOI: https://doi.org/10.1016/j.anireprosci.2004.09.006

Wehrman M E, Welsh T H, Jr. and Williams G L. 1991. Dietinduced hyperlipidemia in cattle modifies the intrafollicular cholesterol environment, modulates ovarian follicular dynamics, and hastens the onset of postpartum luteal activity. Biology of Reproduction 45 (3): 514–22. DOI: https://doi.org/10.1095/biolreprod45.3.514

Wu D and Cederbaum A I. 2000. Ethanol and arachidonic acid produce toxicity in hepatocytes from pyrazole-treated rats with high levels of CYP2E1. Molecular and Cellular Biochemistry 204 (1–2): 157–67.

Yao J K, Ryan R J and Dyck P J. 1980. The porcine ovarian follicle. VI. Comparison of fatty acid composition of serum and follicular fluid at different developmental stages. Biology of Reproduction 22 (2): 141–47. DOI: https://doi.org/10.1095/biolreprod22.2.141