A native whole cell lysate antigen (WCLA) based ELISA for the sero-detection of surra in Indian cattle


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Authors

  • VIKRANT SUDAN Department of Parasitology, College of Veterinary Sciences and Animal Husbandry, UP Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan, Mathura
  • A K TEWARI Principal Scientist, Division of Parasitology, ICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 India
  • HARKIRAT SINGH Department of Veterinary Parasitology, College of Veterinsaary Science, GADVASU, Ludhiana

https://doi.org/10.56093/ijans.v85i6.49299

Keywords:

ELISA, Native whole cell lysate antigen (WCLA), Trypanosoma evansi

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References

Atarhouch T, Rami M, Bendahman M N and Dakkak A. 2003. Camel trypanosomosis in Morocco 1: results of a first epidemiological survey. Veterinary Parasitology 111: 277–86. DOI: https://doi.org/10.1016/S0304-4017(02)00382-5

Kundu K, Tewari A K, Kurup S P, Baidya S, Rao J R and Joshi P. 2013. Sero-surveillance for surra in cattle using native surface glycoprotein antigen from Trypanosoma evansi. Veterinary Parasitology http://dx.doi.org/10.1016/j.vetpar.2013.04.013 DOI: https://doi.org/10.1016/j.vetpar.2013.04.013

Lowry O H, Rosebrough N J, Farr A L and Randall R J. 1951. Protein measurement with the Folin-Phenol reagents. Journal of Biological Chemistry 193: 265–75. DOI: https://doi.org/10.1016/S0021-9258(19)52451-6

Luckins A G. 1977. Detection of antibodies in trypanosomeinfectedcattle by means of microplate enzyme-linked immunosorbent assay. Tropical Animal Health and Production 9: 53–62. DOI: https://doi.org/10.1007/BF02297393

Monzon C M, Mancebo O A and Russo A M. 2003. Antibody levels inindirect ELISA test in Trypanosoma evansi infected horses following treatment with Quinapyramine sulphate. Veterinary Parasitology 111: 59–63. DOI: https://doi.org/10.1016/S0304-4017(02)00331-X

OIE. 2004. Trypanosoma evansi infection (Surra). OIE Manual of Standards for Diagnostic Tests and Vaccines for Terrestrial Animals. World Organisation of Animal Health (Ed.), Paris, France (Chapter 2.1.17.).

Sudan V, Jaiswal A K, Parashar and Shanker D. 2014. A nested polymerase chain reaction based assay for sensitive diagnosis of Trypanosoma evansi infection in water buffaloes. Indian Journal of Animal Sciences 84 (12): 26–30.

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Submitted

2015-06-11

Published

2015-06-11

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Short-Communication

How to Cite

SUDAN, V., TEWARI, A. K., & SINGH, H. (2015). A native whole cell lysate antigen (WCLA) based ELISA for the sero-detection of surra in Indian cattle. The Indian Journal of Animal Sciences, 85(6), 601–603. https://doi.org/10.56093/ijans.v85i6.49299
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