A native whole cell lysate antigen (WCLA) based ELISA for the sero-detection of surra in Indian cattle

Abstract views: 119 / PDF downloads: 15


  • VIKRANT SUDAN Department of Parasitology, College of Veterinary Sciences and Animal Husbandry, UP Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan, Mathura
  • A K TEWARI Principal Scientist, Division of Parasitology, ICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 India
  • HARKIRAT SINGH Department of Veterinary Parasitology, College of Veterinsaary Science, GADVASU, Ludhiana



ELISA, Native whole cell lysate antigen (WCLA), Trypanosoma evansi


Download data is not yet available.


Atarhouch T, Rami M, Bendahman M N and Dakkak A. 2003. Camel trypanosomosis in Morocco 1: results of a first epidemiological survey. Veterinary Parasitology 111: 277–86. DOI: https://doi.org/10.1016/S0304-4017(02)00382-5

Kundu K, Tewari A K, Kurup S P, Baidya S, Rao J R and Joshi P. 2013. Sero-surveillance for surra in cattle using native surface glycoprotein antigen from Trypanosoma evansi. Veterinary Parasitology http://dx.doi.org/10.1016/j.vetpar.2013.04.013 DOI: https://doi.org/10.1016/j.vetpar.2013.04.013

Lowry O H, Rosebrough N J, Farr A L and Randall R J. 1951. Protein measurement with the Folin-Phenol reagents. Journal of Biological Chemistry 193: 265–75. DOI: https://doi.org/10.1016/S0021-9258(19)52451-6

Luckins A G. 1977. Detection of antibodies in trypanosomeinfectedcattle by means of microplate enzyme-linked immunosorbent assay. Tropical Animal Health and Production 9: 53–62. DOI: https://doi.org/10.1007/BF02297393

Monzon C M, Mancebo O A and Russo A M. 2003. Antibody levels inindirect ELISA test in Trypanosoma evansi infected horses following treatment with Quinapyramine sulphate. Veterinary Parasitology 111: 59–63. DOI: https://doi.org/10.1016/S0304-4017(02)00331-X

OIE. 2004. Trypanosoma evansi infection (Surra). OIE Manual of Standards for Diagnostic Tests and Vaccines for Terrestrial Animals. World Organisation of Animal Health (Ed.), Paris, France (Chapter 2.1.17.).

Sudan V, Jaiswal A K, Parashar and Shanker D. 2014. A nested polymerase chain reaction based assay for sensitive diagnosis of Trypanosoma evansi infection in water buffaloes. Indian Journal of Animal Sciences 84 (12): 26–30.









How to Cite

SUDAN, V., TEWARI, A. K., & SINGH, H. (2015). A native whole cell lysate antigen (WCLA) based ELISA for the sero-detection of surra in Indian cattle. The Indian Journal of Animal Sciences, 85(6), 601–603. https://doi.org/10.56093/ijans.v85i6.49299