Effect of graded concentration of organic zinc (zinc glycinate) on skin quality, hematological and serum biochemical constituents in broiler chicken

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  • K SRIDHAR PhD student, College of Veterinary Science, Rajendranagar, Hyderabad, Telangana 500 030 India
  • D NAGALAKSHMI Professor and Head, Department of Animal Nutrition, College of Veterinary Science, Rajendranagar, Hyderabad, Telangana 500 030 India
  • S V RAMA RAO Principal Scientist, Directorate of Poultry Research. College of Veterinary Science, Rajendranagar, Hyderabad, Telangana 500 030 India



Hematology, Serum biochemical constituents, Skin quality, Zinc glycinate


Day-old chicks (120) were randomly distributed to 4 dietary groups with 6 replicate in each and raised for 42 days under uniform managemental conditions to determine the effect of supplementing organic zinc (zinc glycinate, Zn-gly) at lower levels on skin quality, haematological and serum biochemical constituents. Dietary treatments consisted of adding 30, 20 and 10 ppm Zn from Zinc glycinate and 40 ppm Zn (NRC 1994) from ZnSO4 (inorganic) (control) to a corn-soybean meal basal diet prepared as per NRC (1994) nutritional requirements, except Zn. Feed and water was provided ad-libitum. Haematological and serum biochemical constituents were estimated in blood collected at 35 d. At the end of experiment, representative samples of skin were collected from slaughtered birds for histological examination. Haematological constituents (WBC, RBC, Hb concentration, PCV, MCV and lymphocyte concentration), serum total protein, albumin and glucose concentrations did not vary with concentration (10–40 ppm) and source (ZnSO4 or Zn-gly) of Zn supplementation in diet. The cholesterol and albumin to globulin ratio was lowest (P<0.05), while globulin, Zn concentrations and alkaline phosphatase (ALP) activity in serum was highest (P<0.05) at 30 ppm Zn supplementation from Zn- gly compared to other groups and these attributes were comparable between 20 ppm Zn as Zn-gly and 40 ppm Zn as ZnSO4. An increased epithelial cell layer and collagen content was observed with 30 ppm Zn supplementation as Zn-gly compared to other dietary treatments. While epithelium cell layers and collagen content was moderate with addition of 40 ppm Zn as ZnSO4 or 20 ppm Zn as Zn-gly. The skin of birds supplemented with 10 ppm Zn from Zn-gly had few epithelial layers and low amount of collagen. Higher ALP activity indicating higher bioavailability, better skin quality and globulin concentration in serum was observed in birds fed 30 ppm Zn from Zn-gly compared to 40 ppm Zn supplemented from inorganic source. Reducing the dietary Zn supplementation by 50% (20 ppm) from organic Zn had comparable skin quality, serum ALP activity and globulin concentration as 100% (40ppm) supplementation from inorganic source.


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Al-Daraji H J and Amen M H M. 2011. Effect of dietary zinc on certain blood traits of broiler breeder chickens. International Journal of Poultry Research 10: 807–13. DOI: https://doi.org/10.3923/ijps.2011.807.813

Ao T, Pierce J L, Power R, Pescatore A J, Cantor A H, Dawson K A and Ford M J. 2009. Effect of different forms of zinc and copper on the performance and tissue mineral content of chicks. Poultry Science 88: 2171–75. DOI: https://doi.org/10.3382/ps.2009-00117

Ao T, Pierce J L, Power R, Pescatore A J, Cantor A H, Dawson K A, Ford M J and Paul M. 2011. Effects of feeding different concentration and forms of zinc on the performance and tissue mineral status of broiler chicks. British Poultry Science 52: 466–71. DOI: https://doi.org/10.1080/00071668.2011.588198

Arenza J S, Hathi S D, Singh B and Verma P N. 1977. Status of some microminerals in neonatal buffalo calves and their mothers. Indian Journal of Dairy Science 30: 255.

Baroni A, Buommino E, De Gregorio V, Ruocco E, Ruocco V and Wolf R. 2012. Structure and function of the epidermis related to barrier properties. Clinics in dermatology 30 (3): 257–62. DOI: https://doi.org/10.1016/j.clindermatol.2011.08.007

Bolkent S, Yanardag R, Bolkent S, Mutlu O, Yildirim S, Kangawa K, Minegishi Y and Suzuki H. 2006. The effect of zinc supplementation on ghrelin-immunoreactive cells and lipid parameters in gastrointestinal tissue of streptozotocin-induced female diabetic rats. Molecular and Cellular Biochemistry 286: 77–85. DOI: https://doi.org/10.1007/s11010-005-9095-1

Bhowmik D and Chiranjib K P. 2010. A potential medicinal importance of zinc in human health and chronic. International Journal of Pharmacology 1: 05–11.

Burns R B. 1983. Antibody production suppressed in the domestic fowl (Gallus domesticus) by zinc deficiency. Avian Pathology 12: 141–46. DOI: https://doi.org/10.1080/03079458308436156

Close W H. 1999 Organic minerals for pigs: An update pp 51–60 in biotechnology in feed industry. Alltech’s 15th annual symposium. Nottingham Univ.Press.UK.

Cooper G R and Mc Daniel V. 1970. Assay methods. Standard Methods for Clinical Chemistry. 159 –70. (Ed.) Mc Donald R P. John Wiley and Sons, New York.

Costa M A G. Maio/Junho. 2005. O papel do zinco e docromo no desempenho das porcas. Porkworld (ano 4, no. 26): 58.

Coulston L and Dandona P. 1980. Insulin like effects of zinc on adipocytes. Diabetes 29: 665–67. DOI: https://doi.org/10.2337/diabetes.29.8.665

Dardenne M. 2002. Zinc and immune function. European Journal of Clinical Nutrition 56: S20–3. DOI: https://doi.org/10.1038/sj.ejcn.1601479

Duncan D B. 1955. Multiple ‘F’ test, Biometrics 1: 142.

Feng J, Ma W Q, Niu H H, Wu X M, Wang Y and Feng J. 2010. Effects of zinc glycine chelate on growth, hematological, and immunological characteristics in broilers. Biololgical Trace Elements Research 133: 203–11. DOI: https://doi.org/10.1007/s12011-009-8431-9

Gallo R L and Hooper L V. 2012. Epithelial antimicrobial defence of the skin and intestine. Nature Reviews Immunology 12 (7): 503–16. DOI: https://doi.org/10.1038/nri3228

Gustafsson E J. 1976. Improved specificity of serum albumin determination and estimation of acute phase of reactants by use of the bromocresol green. Quinchemisty 22: 616–22. DOI: https://doi.org/10.1093/clinchem/22.5.616

Idowu O M O, Ajuwon R O, Oso A O and Akiniloye O A. 2011. Effects of zinc supplementation on laying performance, serum chemistry and Zn residue in tibia, bone, liver, excreta and egg shell of laying hens. International Journal of Poultry Science 10: 225–30. DOI: https://doi.org/10.3923/ijps.2011.225.230

Jahanian R, Moghaddam H N and Rezaei A. 2008. Improved broiler chick performance by dietary supplementation of organic zinc sources. Asian-Australasian Journal of Animal Sciences 21: 1384–54. DOI: https://doi.org/10.5713/ajas.2008.70699

Kind P R and King E J. 1954. Estimation of plasma phosphatase by determination of hydrolyzed phenol with amino-antipyrine. Journal of Clinical Pathology 7: 322–26. DOI: https://doi.org/10.1136/jcp.7.4.322

Kratzer F H and Vohra P. 1986. Chelate Nutrition. CRC, Boca Raton.

Ledoux D R, Henry P R, Ammerman C B, Rao P V and Miles R D. 1991. Estimation of the relative bioavailability of inorganic copper sources for chicks using tissue uptake of copper. Journal of Animal Science 69: 215–22. DOI: https://doi.org/10.2527/1991.691215x

May J M and Contoreggi C S. 1982. The mechanism of insulin like effects of ionic zinc. Journal of Biological Chemistry 257: 4362–68. DOI: https://doi.org/10.1016/S0021-9258(18)34730-6

Mc Dowell L R. 2003. Zinc. Minerals in Animal and Human Nutrition. pp. 357–96. Elsevier Science, Amsterdam, the Netherlands. DOI: https://doi.org/10.1016/B978-0-444-51367-0.50015-5

Miller J K and Cragle R G. 1965. Gastrointestinal sites of absorption and endogenous secretion of zinc in dairy cattle. Journal of Dairy Science 48: 370–73. DOI: https://doi.org/10.3168/jds.S0022-0302(65)88231-5

National Research Council. 1994. Nutrient requirements of poultry, 9th edition, National Academy Press, Washington.

Parák T and Straková E. 2011. Zinc as a feed supplement and its impact on plasma cholesterol concentrations in breeding cocks. Acta Veterinaria Brno 80: 281–85. DOI: https://doi.org/10.2754/avb201180030281

Rashtchizadeh N, Ettehad S, Disilvestro R A and Mahdavi R. 2008. Antiatherogenic effects of zinc are associated with copper in iron-overloaded hypercholesterolemic rabbits. Nutritional Research 28: 98–105. DOI: https://doi.org/10.1016/j.nutres.2007.12.004

Reinhold J G. 1953. Standard Methods of Clinical Chemistry C, (Ed.) Rynner, M, New York, Academic Press pp 88.

Reiterer G, Macdonald R, Browning J D, Morrow J, Matveev S V, Daugherty A, Smart E, Toborek M and Hennig B. 2005. Zinc deficiency increases plasma lipids and atherosclerotic markers in LDL-receptor-deficient mice. Journal of Nutrition 135: 2114–18. DOI: https://doi.org/10.1093/jn/135.9.2114

Revy P S, Jondreville C, Dourmad J Y, Guinotte F and Nys Y. 2002. Bioavailability of two sources of zinc in weanling pigs. Animal Research 51: 315–26. DOI: https://doi.org/10.1051/animres:2002028

Rossi P, Rutz F, Amciuti M A, Rech J L and Zauk N H F. 2007. Influence of graded levels of organic zinc on growth performance and carcass traits of broilers. Journal of Applied Poultry Research 16: 219–25. DOI: https://doi.org/10.1093/japr/16.2.219

Sahin K, Smith M O, Onderci M, Sahin N, Gursu M F and Kucuk O. 2005. Supplementation of zinc from organic or inorganic source improves performance and antioxidant status of heat-distressed quail. Poultry Science 84: 882–87. DOI: https://doi.org/10.1093/ps/84.6.882

Salim H M, Lee H R, Jo C, Lee S K and Lee B D. 2011. Supplementation of graded levels of organic zinc in the diets of female broilers: effects on performance and carcass quality. British Poultry Science 52: 606–12. DOI: https://doi.org/10.1080/00071668.2011.616485

Shisheva A, Gefel D and Shechter Y. 1992. Insulin like effects of zinc ion in vitro and in vivo: preferential effects on desensitized adipocytes and induction of normoglycemia in streptozocininduced rats. Diabetes 41: 982–88. DOI: https://doi.org/10.2337/diabetes.41.8.982

Snedecor G W and Cochran W G. 1994. Statistical methods, 8th ed., (Iowa State University Press, Ames, Iowa, USA).

Someya Y, Ichinose T, Sachiko Nomura, Kawashima Yu, Sugiyama M, Tachiyashiki K and Imaizumi K. 2007. Effects of zinc deficiency on the number of white blood cells in rats. The FASEB Journal 21: A719. DOI: https://doi.org/10.1096/fasebj.21.5.A719-a

Wang Y, Tang J W, Ma W Q, Feng J and Feng J. 2010. Dietary zinc glycine chelate on growth performance, tissue mineral concentration, and serum enzyme activity in weanling piglets. Biological Trace Element Research 133: 325–34. DOI: https://doi.org/10.1007/s12011-009-8437-3

Wybenga D R, Pileggi V J, Dirstine P H and Di Giorgio J. 1970. Direct manual determination of serum total cholesterol with a single stable reagent. Clinical Chemistry 16: 980–84. DOI: https://doi.org/10.1093/clinchem/16.12.980

Zhao J, Shirley R B, Vazque-Anon M, Dibner J, Richards J D, Fishe P, Hampton T, Christensen K D, Allard J P and Giesen A F. 2010. Effects of chelated trace minerals on growth performance, breast meat yield, and footpad health in commercial meat broilers. Journal of Applied Poultry Research 19: 365–72. DOI: https://doi.org/10.3382/japr.2009-00020









How to Cite

SRIDHAR, K., NAGALAKSHMI, D., & RAO, S. V. R. (2015). Effect of graded concentration of organic zinc (zinc glycinate) on skin quality, hematological and serum biochemical constituents in broiler chicken. The Indian Journal of Animal Sciences, 85(6), 643–648. https://doi.org/10.56093/ijans.v85i6.49335