Phytochemical screening for antimicrobial and antioxidant activity of periwinkle – Manish Kapoor, Jyoti Rani, Ajit Kumar and Sanchita Ghosh

Authors

  • Manish Kapoor

Abstract

To perform preliminary phytochemical screening, investigated total flavonoids, phenolic, antioxidant and to evaluate antimicrobial potential of Catharanthus roseus, chemical composition of the various crude extracts from the leaves was determined by GC-MS analysis. The dried leaves of C. roseus were powdered and subjected to extraction using three different solvents. All prepared extracts were then subjected to detect flavonoid and phenolic contents as per Folin-Ciocalteu and aluminium chloride methods, respectively. Various assays like SOD, POD, CAT, ABTS and DPPH were used to analyse in vitro antioxidant activity. All plant extracts were further used to determine antimicrobial activity against five selected bacterial strains. Each of extract was subjected further to GC-MS. Preliminary phytochemical analysis showed that the maximum phytoconstituents were found in methanolic extract. The lowest IC50 value was exhibited by methanolic extract followed by ethanolic extract. The activity levels of antioxidant enzymes of the plant extracts were found to be satisfactory. The antimicrobial potential of the plant extracts was found to be positively correlated with the total phenolics and flavonoid contents. Fifteen different phytoconstituents in methanolic extract, seven in ethanolic extract, while minimum five compounds were identified in the petroleum ether extract by GC-MS. Some of these compounds are known to possess antimicrobial and antioxidant properties.

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Published

2021-12-31

How to Cite

Kapoor, M. (2021). Phytochemical screening for antimicrobial and antioxidant activity of periwinkle – Manish Kapoor, Jyoti Rani, Ajit Kumar and Sanchita Ghosh. Indian Journal of Horticulture, 78(4). Retrieved from https://epubs.icar.org.in/index.php/IJH/article/view/121258

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Articles