Serum Biochemical Alterations in Sheep Affected by Ovine Pneumonic Pasteurellosis

Abstract

Ovine Pneumonic Pasteurellosis is one of the most economically important infectious disease in sheep. The infection is caused by group of bacteria belonging to family Pasteurellaceae. In present study, blood samples were collected from ten (n=10) pasteurellosis affected sheep after molecular confirmation through PCR assay using 16S rRNA universal primer. Additionally, blood samples were also collected from ten (n=10) apparently healthy sheep to serve as a control group. The overall mean value of biochemical parameters viz. AST, ALT, calcium, phosphorus, magnesium and CPK were 96.11 ± 0.96 IU/L, 62.61 ± 2.77 IU/L, 10.99 ± 0.25 mg/dL, 7.60 ± 0.20 mg/dL, 1.90 ± 0.86 mg/dL and 9.31 ± 0.23 IU/L, respectively, which were obtained from Pasteurellosis affected sheep. Affected sheep exhibited significantly (p<0.01) elevated serum AST and ALT levels as compared to healthy sheep. Serum calcium and magnesium levels were significantly lower (p<0.05 and p<0.01, respectively), while phosphorus levels were significantly higher (p<0.05) in affected sheep. Serum CPK levels were also found higher in affected sheep although this change was non-significant. This study highlights significant alterations in serum biochemical parameters in sheep affected by Pneumonic Pasteurellosis. Elevated AST, ALT and phosphorus, along with reduced calcium and magnesium in affected sheep indicate the systemic impact of the infection. These findings contribute to understanding the patho-physiological effects of the disease and may assist in diagnostic and therapeutic approaches in pasteurellosis affected sheep.