Differential expression profiles of Toll like receptors 20 and 21 in Heteropneustes fossilis in response to infection with Aeromonas hydrophila
81 / 0
Authors
-
Aftabuddin Md
-
Tandrima Mitra
-
Arpita Basu
Abstract
The innate immunity serves as the first line of defence against invasion of pathogens in fish. Toll-like receptors (TLRs) play a pivotal role in the innate
immunity by specific pathogen-associated molecules that activate immune responses. In the present study, analysis of TLR 20 and TLR 21 in different tissues of healthy catfish, Heteropneustes fossilis showed that the patterns of the gene expression were in the order of spleen > intestine > head kidney > gill and gill > head kidney > liver > spleen > intestine, respectively. Relative transcription of TLR 20 and TLR 21 in different tissues of the fish against bacterial infection investigated by semi- quantitative RT-PCR showed that expression of both TLR 20 and TLR 21 were up-regulated in spleen and intestine whereas that of TLR 21 expression was down-regulated in kidney, gill and liver relative to control fish. Findings of the study suggested that spleen and intestine showed significant alteration in both TLR 20 and TLR 21 expression. Expression of TLR 21 found to be more sensitive than TLR 20 against bacterial infection in fish.
Key words Heteropneustes fossilis, Aeromonas hydrophila, TLR 20, TLR 21, Gene expression, Semi-quantitative PCR.
References
Akira, S., and Takeda, K. 2004. Toll-like receptor signalling. National Review on Immunology. 4: 499-511
Altschul, S. F., Gish, W., Miller, W., Myers, E. W. and Lipman, D.J. 1990. Basic local alignment search tool. Journal of Molecular Biology. 215: 403-410
Banerjee, S., Mitra, T., Purohit, G. K., Mohanty, S., and Mohanty. B. P. 2015. Immunomodulatory effect of arsenic on cytokine and HSP gene expression in Labeo rohita fingerlings. Fish Shellfish Immunology.
(1): 43-49
Baoprasertkul, P., Xu, P., Peatman, E., Kucuktas, H. and Liu, Z. 2007. Divergent Toll-like receptors in catfish (Ictalurus punctatus): TLR5S, TLR20, TLR21. Fish and Shellfish Immunology. 23: 1218-1230
Brownlie, R., Zhu, J., Allan, B., Mutwiri, G.K., Babiuk, L.A., Potter A. and Griebel, P. 2009. Chicken TLR21 acts as a functional homologue to mammalian TLR9 in the recognition of CpG oligodeoxynucleotides. Molecular Immunology. 246: 3163-3170
Chaturvedi, A. and Pierce. S. K. 2009. How location governs Toll like receptor signalling. Traffic. 10(6): 621-628
Danilo, P., Marleen, S., Inge, R.F., Nico, T., Huub, F.J.S., Herman, P.S., Maria, F. and Geert F.W. 2014.
Identification and functional characterization of nonmammalian Toll-like receptor 20. Immunogenetics.66:123-141
FishBase http://www.fishbase.org/summary/4885 Hawke, J.P., Kent, M., Rogge, M., Baumgartner, W., Wiles, J., Shelley, J., Savolainen, L.C., Wagner, R., Murray, K., and Peterson. T.S. 2013. Edwardsiellosis caused by Edwardsiella ictaluri in laboratory populations of Zebrafish Danio rerio. Journal of Aquatic Animal Healt., 25(3): 171-183
Kawasaki, T. and Kawai, T. 2014. Toll-like receptor signalling pathways. Frontier Immunology. 25(5):461
Lai, Rui-Fang., Jakovlic, I., Liu, H., Zhan, Fan-Bin., Wei, J. and Wang, Wei-Min. 2016. Molecular characterization and immunological response analysis of Toll-like receptors from the blunt snout bream (Megalobrama amblycephala). Developmental and Comparative
Immunology. 67:471-475
Mogensen. T.H. 2009. Pathogen recognition and inflammatory signaling in innate immune defences. Clinical Microbiology Review. 22(2): 240-273
Park, H., Huxley-Jones, J., Boot-Handford, R., Bishop, P., Attwood, T. and Bella, J. 2008. LRRCE: a leucine-rich repeat cysteine capping motif unique to the chordate lineage. BMC Genomics. 9: 599
Rauta, P. R., Samanta, M., Dash, H. R., Nayak, B. and Das, S. 2014. Toll-like receptors (TLRs) in aquatic animals: signaling pathways, expressions and immune responses. Immunology Letters. 158(2): 14-24
Wang, P., Zhao, C., Wang, C., Fan, S., Yan, L. and Qiu. L. 2018.
TLR 3 gene in Japanese sea perch (Lateolabrax japonicus): Molecular cloning, characterization and expression analysis after bacterial infection. Fish and Shellfish Immunolog. 76: 347-354
Zhao, C., Fu, M., Wang, C., Jiao, Z. and Qiu, L. 2016. RNA Seq analysis of immune-relevant genes in Lateolabrax japonicus during Vibrio anguillarum infection. Fish Shell￾fish Immunology. 52(370): 57-64
Zhao, F., Li, Yan-Wei., Pan, Hou-Jun., Shi, Cun-Bin., Luo, Xiao-Chun, Li, An-Xing. and Wu, Shu-Qin. 2013.
Expression profiles of toll-like receptors in channel catfish (Ictalurus punctatus) after infection with Ichthyophthirius multifiliis. Fish and Shellfish Immunology. 35: 993-997
Downloads
Submitted
Published
Issue
Section
License
In case of publication of the article in the journal, author(s) are required to assign copyright to the Journal of the Inland Fisheries Society of India for its publication in any form/language including all media (print and electronic, or presently unknown), and exclusive right to use the matter for the life of the work (no time restriction on re use of matter).