Bioactivity-guided isolation and structural characterization of endogenously accreted raphide crystals in Ipomoea aquatica Forssk.
215 / 0
Authors
-
N Chakraborty
-
A K Das
-
R K Manna
-
B K Das
-
B Mandal
Abstract
Bio-minerals are one of the secondary plant metabolites that are imperative for their existence. Raphides or calcium oxalate (CaC2O4) crystals are the
most abundantly synthesized bio-minerals in plants which help channelize the excess calcium absorbed. Raphides have been isolated (1.6% dry weight) and purified by chromatographic process from aqueous fraction of Ipomoea aquatica leaves. Direct bio autography followed by agar well diffusion assay reveals the in-vitro antimicrobial property of the crystals against fish pathogen Citrobacter freundii with MIC< 50 ppm. The atomic force microscopy predicts a hydrous inorganic salt displaying average peaks
between 4-5 nm with homogeneity of dispersion. The crystal exhibited moderate level of free radical scavenging activity with IC50 at 0.144 mg/ml. The molecular structure characterization of the crystal by the magnetic resonance and infra-red spectra further confirmed the presence of raphides.
Key words Ipomoea aquatica; Calcium oxalate; Atomic Force Microscopy; Structure characterization; Citrobacter freundii; Antioxidant.
References
1. Abhirama, B.R. and ShanmugaSundaram, R. 2018. Antiurolithic
2. and antioxidant activity of ethanol extract of whole￾plant Biophytum sensitivum (Linn.) DC in Ethylene￾Glycol-induced urolithiasis in rats. Pharmacognosy Re￾search, 10(2):181.
3. Adebiyi, O.E., Olayemi, F.O., Ning-Hua, T. and Guang-Zhi, Z.
4. In vitro antioxidant activity, total phenolic and
5. flavonoid contents of ethanol extract of stem and leaf
6. of Grewia carpinifolia. Journal of Basic and Applied
7. Sciences, 6(1):10-14.
8. Baba, S.A. and Malik, S.A. 2015. Determination of total
9. phenolic and flavonoid content, antimicrobial and
10. antioxidant activity of a root extract of Arisaema
11. jacquemontii Blume. Journal of Taibah University for
12. Science, 9(4):449-454.
13. Bouropoulos, N., Weiner, S. and Addadi, L.2001. Calcium
14. oxalate crystals in tomato and tobacco plants:
15. morphology and in vitro interactions of crystal
16. associated macromolecules. Chemistry-a European
17. Journal, 7(9):1881-1888.
18. Braissant, O., Cailleau, G., Aragno, M. and Verrecchia, E.P. 2004.
19. Biologically induced mineralization in the tree Milicia
20. excelsa (Moraceae): its causes and consequences to the
21. environment. Geobiology, 2(1):59-66.
22. Çalikan, M., 2000. The metabolism of oxalic acid. Turkish
23. Journal of Zoology, 24(1):103-106.
24. Chandra, J. Helan & Shamli. 2015. Antibacterial, Antioxidant
25. and in Silico Study of Ipomoea aquatica Forsk. Journal of
26. Pure and Applied Microbiology. 9: 1371-1376.
27. Choma, I.M. and Grzelak, E.M. 2011. Bioautography detection
28. in thin-layer chromatography. Journal of
29. Chromatography A, 1218(19): 2684-2691.
30. Cie la, L.M., Waksmundzka-Hajnos, M., Wojtunik, K.A. and
31. Hajnos, M. 2015. Thin-layer chromatography coupled
32. with biological detection to screen natural mixtures for
33. potential drug leads. Phytochemistry Letters,
34. :445-454.
35. Dahiya, P. and Purkayastha, S. 2012. Phytochemical screening
36. and antimicrobial activity of some medicinal plants
37. against multi-drug resistant bacteria from clinical
38. isolates. Indian Journal of Pharmaceutical Sciences,
39. (5): 443.
40. Emelda, A. 2015. Polyphenol total content, IC50 and
41. antioxidant activities of ethanol extract from some cocoa
42. (Theobroma cacao) beans in South Sulawesi Indonesia.
43. Journal of Chemical and Pharmaceutical Research,
44. (4):1211-1214.
45. Faheed, F., Mazen, A. and ELMOHSEN, S.A.2013.
46. Physiological and ultrastructural studies on calcium
47. oxalate crystal formation in some plants. Turkish
48. Journal of Botany, 37(1):139-152.
49. Franceschi, V.R. and Horner, H.T. 1980. Calcium oxalate
50. crystals in plants. The Botanical Review, 46(4):361-427.
51. Guo, S., Ward, M.D. and Wesson, J.A. 2002. Direct
52. visualization of calcium oxalate monohydrate
53. crystallization and dissolution with atomic force
54. microscopy and the role of polymeric additives.
55. Langmuir, 18(11):4284-4291.
56. Hartmann, T. 2008. The lost origin of chemical ecology in the
57. late 19th century. Proceedings of the National Academy
58. of Sciences, 105(12):4541-4546.
59. Horner, H.T. and Wagner, B.L. 1995. Calcium oxalate
60. formation in higher plants. In Calcium oxalate in
61. biological systems: 53-72.
62. Horner, H.T. and Whitmoyer, R.E. 1972. Raphide crystal cell
63. development in leaves of Psychotria punctata (Rubiaceae).
64. Journal of Cell Science, 11(2):339-355.
65. Huang, D.J.,Chen, H.J.,Lin, C.D. and Lin,Y.H. 2005.
66. Antioxidant and antiproliferative activities of water
67. spinach (Ipomoea aquatica Forsk) constituents.
68. Botanical bulletin of Academia Sinica, 46: 99-106.
69. Junior, G.B., dos Santos, A.C., de Freitas Souza, C., Baldissera,
70. M.D., dos Santos Moreira, K.L., da Veiga, M.L., de Vargas,
71. A.P.C., da Cunha, M.A. and Baldisserotto, B. 2018.
72. Citrobacter freundii infection in silver catfish (Rhamdia
73. quelen): Hematological and histological alterations.
74. Microbial pathogenesis, 125:276-280.
75. Konno, K., Inoue, T. A. and Nakamura, M. 2014. Synergistic
76. defensive function of raphides and protease through the
77. needle effect. Public Library of Science (PLOS) one,
78. (3).
79. Konyar, S.T., Öztürk, N. and Dane, F. 2014. Occurrence, types
80. and distribution of calcium oxalate crystals in leaves and
81. stems of some species of poisonous plants. Botanical
82. Studies, 55(1):32.
83. Korth, K.L., Doege, S.J., Park, S.-H., Goggin, F.L., Wang, Q.,
84. Gomez, S. K., Liu G., Jia L., and Nakata, P.A. 2006.
85. Medicago truncatula mutants demonstrate the role of plant
86. calcium oxalate crystals as an effective defense against
87. chewing insects. Plant Physiology. 141: 188-195.
88. McNair, J.B.1932. The interrelation between substances in
89. plants: essential oils and resins, cyanogen and oxalate.
90. American Journal of Botany, 19(3):255-272.
91. Nakata, P.A.2003. Advances in our understanding of calcium
92. oxalate crystal formation and function in plants. Plant
93. Science, 164(6):901-909.
94. Parekh, J. and Chanda, V.S. 2007. In vitro Antimicrobial
95. Activity and Phytochemical Analysis of Some Indian
96. Medicinal Plants. Turkish Journal of Biology, 31:53-58.
97. Rehman, J., Khan, I.U. and Asghar, M.N. 2013. Antioxidant
98. activity and GC-MS analysis of Grewia optiva E3.
99. Journal of Biotechnology and Pharmaceutical Research,
100. (1):14-21.
101. Stahl, E. 1919.The physiology and biology of excretion. Flora,
102. : 1-192.
103. Subashini, S. and Kumar, K. S. 2017. Physicochemical
104. characteristics of calcium oxalate crystals in Spinacia
105. oleracea L. Indian journal of Biochemistry and
106. Biophysics, 54:156-163.
107. Suleiman, M.M., McGaw, L.I., Naidoo, V. and Eloff, J. 2010.
108. Detection of antimicrobial compounds by
109. bioautography of different extracts of leaves of selected
110. South African tree species. African Journal of Traditional,
111. Complementary and Alternative Medicines, 7(1).
112. Thi, A.N.T., Noseda, B., Samapundo, S., Nguyen, B.L., Broekaert,
113. K., Rasschaert, G., Heyndrickx, M. and Devlieghere, F.
114. Microbial ecology of Vietnamese Tra fish
115. (Pangasius hypophthalmus) fillets during processing.
116. International Journal of Food Microbiology,
117. (2):144-152.
118. Tilton, V.R. and Horner Jr, H.T.1980. Calcium oxalate raphide
119. crystals and crystalliferous idioblasts in the carpels of
120. Ornithogalum caudatum. Annals of Botany,
121. (5):533-539.
122. Tooulakou, G., Giannopoulos, A., Nikolopoulos, D., Bresta, P.,
123. Dotsika, E., Orkoula, M.G., Kontoyiannis, C.G., Fasseas,
124. C., Liakopoulos, G., Klapa, M.I. and Karabourniotis, G.
125. " Alarm photosynthesis": calcium oxalate crystals
126. as an internal CO2 source in plants. Plant Physiology,
127.
128. Webb, M.A.1999. Cell-mediated crystallization of calcium
129. oxalate in plants. The Plant Cell, 11(4):751-761.
130. Zindler-Frank, E. 1976. Oxalate biosynthesis in relation to
131. photosynthetic pathway and plant productivity-a
132. survey. Zeitschrift für Pflanzenphysiologie,
133. (1):1-13.
Downloads
Published
Issue
Section
License
In case of publication of the article in the journal, author(s) are required to assign copyright to the Journal of the Inland Fisheries Society of India for its publication in any form/language including all media (print and electronic, or presently unknown), and exclusive right to use the matter for the life of the work (no time restriction on re use of matter).