Bioactivity-guided isolation and structural characterization of endogenously accreted raphide crystals in Ipomoea aquatica Forssk.
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Authors
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N Chakraborty
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A K Das
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R K Manna
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B K Das
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B Mandal
Abstract
Bio-minerals are one of the secondary plant metabolites that are imperative for their existence. Raphides or calcium oxalate (CaC2O4) crystals are the
most abundantly synthesized bio-minerals in plants which help channelize the excess calcium absorbed. Raphides have been isolated (1.6% dry weight) and purified by chromatographic process from aqueous fraction of Ipomoea aquatica leaves. Direct bio autography followed by agar well diffusion assay reveals the in-vitro antimicrobial property of the crystals against fish pathogen Citrobacter freundii with MIC< 50 ppm. The atomic force microscopy predicts a hydrous inorganic salt displaying average peaks
between 4-5 nm with homogeneity of dispersion. The crystal exhibited moderate level of free radical scavenging activity with IC50 at 0.144 mg/ml. The molecular structure characterization of the crystal by the magnetic resonance and infra-red spectra further confirmed the presence of raphides.
Key words Ipomoea aquatica; Calcium oxalate; Atomic Force Microscopy; Structure characterization; Citrobacter freundii; Antioxidant.
References
Abhirama, B.R. and ShanmugaSundaram, R. 2018. Antiurolithic
and antioxidant activity of ethanol extract of whole￾plant Biophytum sensitivum (Linn.) DC in Ethylene￾Glycol-induced urolithiasis in rats. Pharmacognosy Re￾search, 10(2):181.
Adebiyi, O.E., Olayemi, F.O., Ning-Hua, T. and Guang-Zhi, Z.
In vitro antioxidant activity, total phenolic and
flavonoid contents of ethanol extract of stem and leaf
of Grewia carpinifolia. Journal of Basic and Applied
Sciences, 6(1):10-14.
Baba, S.A. and Malik, S.A. 2015. Determination of total
phenolic and flavonoid content, antimicrobial and
antioxidant activity of a root extract of Arisaema
jacquemontii Blume. Journal of Taibah University for
Science, 9(4):449-454.
Bouropoulos, N., Weiner, S. and Addadi, L.2001. Calcium
oxalate crystals in tomato and tobacco plants:
morphology and in vitro interactions of crystal
associated macromolecules. Chemistry-a European
Journal, 7(9):1881-1888.
Braissant, O., Cailleau, G., Aragno, M. and Verrecchia, E.P. 2004.
Biologically induced mineralization in the tree Milicia
excelsa (Moraceae): its causes and consequences to the
environment. Geobiology, 2(1):59-66.
Çalikan, M., 2000. The metabolism of oxalic acid. Turkish
Journal of Zoology, 24(1):103-106.
Chandra, J. Helan & Shamli. 2015. Antibacterial, Antioxidant
and in Silico Study of Ipomoea aquatica Forsk. Journal of
Pure and Applied Microbiology. 9: 1371-1376.
Choma, I.M. and Grzelak, E.M. 2011. Bioautography detection
in thin-layer chromatography. Journal of
Chromatography A, 1218(19): 2684-2691.
Cie la, L.M., Waksmundzka-Hajnos, M., Wojtunik, K.A. and
Hajnos, M. 2015. Thin-layer chromatography coupled
with biological detection to screen natural mixtures for
potential drug leads. Phytochemistry Letters,
:445-454.
Dahiya, P. and Purkayastha, S. 2012. Phytochemical screening
and antimicrobial activity of some medicinal plants
against multi-drug resistant bacteria from clinical
isolates. Indian Journal of Pharmaceutical Sciences,
(5): 443.
Emelda, A. 2015. Polyphenol total content, IC50 and
antioxidant activities of ethanol extract from some cocoa
(Theobroma cacao) beans in South Sulawesi Indonesia.
Journal of Chemical and Pharmaceutical Research,
(4):1211-1214.
Faheed, F., Mazen, A. and ELMOHSEN, S.A.2013.
Physiological and ultrastructural studies on calcium
oxalate crystal formation in some plants. Turkish
Journal of Botany, 37(1):139-152.
Franceschi, V.R. and Horner, H.T. 1980. Calcium oxalate
crystals in plants. The Botanical Review, 46(4):361-427.
Guo, S., Ward, M.D. and Wesson, J.A. 2002. Direct
visualization of calcium oxalate monohydrate
crystallization and dissolution with atomic force
microscopy and the role of polymeric additives.
Langmuir, 18(11):4284-4291.
Hartmann, T. 2008. The lost origin of chemical ecology in the
late 19th century. Proceedings of the National Academy
of Sciences, 105(12):4541-4546.
Horner, H.T. and Wagner, B.L. 1995. Calcium oxalate
formation in higher plants. In Calcium oxalate in
biological systems: 53-72.
Horner, H.T. and Whitmoyer, R.E. 1972. Raphide crystal cell
development in leaves of Psychotria punctata (Rubiaceae).
Journal of Cell Science, 11(2):339-355.
Huang, D.J.,Chen, H.J.,Lin, C.D. and Lin,Y.H. 2005.
Antioxidant and antiproliferative activities of water
spinach (Ipomoea aquatica Forsk) constituents.
Botanical bulletin of Academia Sinica, 46: 99-106.
Junior, G.B., dos Santos, A.C., de Freitas Souza, C., Baldissera,
M.D., dos Santos Moreira, K.L., da Veiga, M.L., de Vargas,
A.P.C., da Cunha, M.A. and Baldisserotto, B. 2018.
Citrobacter freundii infection in silver catfish (Rhamdia
quelen): Hematological and histological alterations.
Microbial pathogenesis, 125:276-280.
Konno, K., Inoue, T. A. and Nakamura, M. 2014. Synergistic
defensive function of raphides and protease through the
needle effect. Public Library of Science (PLOS) one,
(3).
Konyar, S.T., Öztürk, N. and Dane, F. 2014. Occurrence, types
and distribution of calcium oxalate crystals in leaves and
stems of some species of poisonous plants. Botanical
Studies, 55(1):32.
Korth, K.L., Doege, S.J., Park, S.-H., Goggin, F.L., Wang, Q.,
Gomez, S. K., Liu G., Jia L., and Nakata, P.A. 2006.
Medicago truncatula mutants demonstrate the role of plant
calcium oxalate crystals as an effective defense against
chewing insects. Plant Physiology. 141: 188-195.
McNair, J.B.1932. The interrelation between substances in
plants: essential oils and resins, cyanogen and oxalate.
American Journal of Botany, 19(3):255-272.
Nakata, P.A.2003. Advances in our understanding of calcium
oxalate crystal formation and function in plants. Plant
Science, 164(6):901-909.
Parekh, J. and Chanda, V.S. 2007. In vitro Antimicrobial
Activity and Phytochemical Analysis of Some Indian
Medicinal Plants. Turkish Journal of Biology, 31:53-58.
Rehman, J., Khan, I.U. and Asghar, M.N. 2013. Antioxidant
activity and GC-MS analysis of Grewia optiva E3.
Journal of Biotechnology and Pharmaceutical Research,
(1):14-21.
Stahl, E. 1919.The physiology and biology of excretion. Flora,
: 1-192.
Subashini, S. and Kumar, K. S. 2017. Physicochemical
characteristics of calcium oxalate crystals in Spinacia
oleracea L. Indian journal of Biochemistry and
Biophysics, 54:156-163.
Suleiman, M.M., McGaw, L.I., Naidoo, V. and Eloff, J. 2010.
Detection of antimicrobial compounds by
bioautography of different extracts of leaves of selected
South African tree species. African Journal of Traditional,
Complementary and Alternative Medicines, 7(1).
Thi, A.N.T., Noseda, B., Samapundo, S., Nguyen, B.L., Broekaert,
K., Rasschaert, G., Heyndrickx, M. and Devlieghere, F.
Microbial ecology of Vietnamese Tra fish
(Pangasius hypophthalmus) fillets during processing.
International Journal of Food Microbiology,
(2):144-152.
Tilton, V.R. and Horner Jr, H.T.1980. Calcium oxalate raphide
crystals and crystalliferous idioblasts in the carpels of
Ornithogalum caudatum. Annals of Botany,
(5):533-539.
Tooulakou, G., Giannopoulos, A., Nikolopoulos, D., Bresta, P.,
Dotsika, E., Orkoula, M.G., Kontoyiannis, C.G., Fasseas,
C., Liakopoulos, G., Klapa, M.I. and Karabourniotis, G.
" Alarm photosynthesis": calcium oxalate crystals
as an internal CO2 source in plants. Plant Physiology,
Webb, M.A.1999. Cell-mediated crystallization of calcium
oxalate in plants. The Plant Cell, 11(4):751-761.
Zindler-Frank, E. 1976. Oxalate biosynthesis in relation to
photosynthetic pathway and plant productivity-a
survey. Zeitschrift für Pflanzenphysiologie,
(1):1-13.
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