DIGESTIVE AND METABOLIC RESPONSES TOWARDS DIET OPTIMIZATION IN LABEO ROHITA
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Authors
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D. DEBNATH
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A. K. PAL
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N. P. SAHU
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S. YENGKOKPAM
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K. SUKUMARAN
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K. K. JAIN
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K. BARUAH
Abstract
Digestive and metabolic responses of Labeo rohita (av. wt. 1.07–1.14 g) to different dietary crude protein (CP) levels ( 25, 30, 35, 40 and 45%) were studied to optimize a practical diet formulation for this species. Soybean meal and fish meal were served as the main protein sources, whereas corn flour and rice polish as major carbohydrate sources. After 45 days of feeding, trypsin and chymotrypsin activities were lower in 25% CP group. Alkaline phosphatase and trypsin activities were maximized (p<0.05) in 35% CP group. Amylase activity was not influenced by the diet composition. Hexokinase (HK) activity in the muscle of fish fed 25 to 35% CP was significantly higher (p<0.05) than the other two groups. Liver also recorded a higher HK activity in low protein fed groups. Similarly, muscle and liver pyruvate kinase activities were significantly higher in 25 and 30% CP fed groups. Malate dehydrogenase activity in muscle was highest at 30% CP group. Muscle aspartate amino-transferase activity was found to be higher in low protein fed groups ( 25 and 30%). Metabolites showed significant change corresponding to the dietary composition. Glucose and glycogen levels matched well with the dietary carbohydrate levels. Muscle and plasma pyruvic acid contents increased as the dietary protein increased, whereas liver pyruvic acid showed opposite trend. Protein fractions in plasma (total protein, albumin and globulin) showed maximum values in 30% CP group.
Key words: Crude protein, digestive enzymes, metabolic enzymes, metabolites, Labeo rohita
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