DEVELOPMENT OF EIGHT EST-DERIVED MICROSATELLITE MARKERS IN INDIAN SNOW TROUT (SCHIZOTHORAX RICHARDSONII GRAY 1832)
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Authors
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A. BARAT
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S. SHARMA
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R. MATURA
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P. C. MAHANTA
Abstract
Indian snow trout, Schizothorax richardsonii is distributed in fast flowing torrential snow-fed streams, rivers and lakes in various altitudes of the Himalayas. Developed fifteen microsatellite markers from approximately 32923-expressed sequence tags (ESTs) of common carp, Cyprinus carpio and evaluated eight of them in 42 unrelated specimens of S. richardsonii. The number of alleles per locus ranged from 3 to 6. The observed heterozygosity (Ho ) ranged from 0.099-0.937 and the expected heterozygosity (He ) ranged from 0.675-0.755. Because of high level of polymorphism the microsatellite markers reported here would be useful to study population genetics and linkage mapping of Indian snow trout.
Keywords: Indian snow trout, Schizothorax richardsonii, expressed sequence tag (EST), microsatellite markers
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