ANTIMICROBIAL SENSITIVITY OF BACTERIAL FLORA ASSOCIATED WITH SEPTICAEMIC VIBRIOSIS IN TIGER SHRIMP, PENAEUS MONODON
128
Authors
-
S. S. MISHRA
-
M. DHIMAN
-
PRIYANKA DAS
-
P. MALI
-
M. K. DAS
Abstract
Among the groups of microorganisms affecting shrimp culture, vibrios are the best known bacteria responsible for devastating economic losses both in shrimp hatchery and culture farms (Lavilla-Pitogo, 1995). Various Vibrio spp. have been implicated in major mortality cases in juvenile penaeid shrimp(Karunasagar et al., 1994) and involved as primary pathogens in shell disease (Lightner, 1996). Vibrio parahaemolyticus and V. alginolyticus have been isolated from cases of septicaemic vibriosis, black spot and shell
necrosis disease in shrimp (Sinderman and Lightner, 1988). Vibrio spp. are regarded as members of the normal bacterial flora of shrimp, often acting as opportunistic pathogens under stressful conditions inflicting mortalities up to 100% (Lightner, 1996). However, other Vibrio spp., Aeromonas spp. and Flavobacterium spp. have been isolated and shown to be involved in disease
conditions of shrimp (Lavilla-Pitogo, 1995; Sinderman and Lightner, 1988). Again V. parahaemolyticus, a common inhabitant of brackishwater and coastal marine water has been of great public health significance (Joseph
et al., 1982), warranting methods for their diagnosis and control. Another aspect, which demands urgent attention is development and transmission of drug resistant strains of bacteria in aquatic ecosystem. The regular and
continuous use of antibiotics in shrimp and fish culture has been associated with increased incidence of antibiotic resistant bacteria (Abraham et al., 1997; Karunasagar et al., 1994; Mohney et al., 1994). The role of
antimicrobial compounds in controlling infectious diseases in cultured fish and shellfish has been well recognized. A wide range of aqua-drugs are being marketed and used in aquaculture. Detailed comparative
studies of different aqua-drugs to assess their anti-bacterial potential and suitability for aquaculture applications are essential (Hameed and Rao, 1994). This communication reports the sensitivity of selected bacteria isolated from diseased shrimp, Penaeus monodon to antibiotics and commercially available aqua-drugs, commonly used in fish and shrimp
culture.Â
References
Abraham, T. J., Manley, R., Palaniappan, R. and Dhevendran, K. 1997. Pathogenicity and antibiotic sensitivity of luminous Vibrio harveyi isolates from diseased penaeid shrimp. J.Aqua.Trop., 12 (1) : 1-8.
Bauer, A. W., Kirby, W. M. M., Sherris, J. C., and Turck, M. 1966. Antibiotic susceptibility testing by a standardized single disc method. Am. J. Clin. Pathol., 45 : 493-496.
Buchanan, R. E. and Gibbons, N. E. 1988. Burgey’s manual of determinative bacteriology, 8th Ed. Williams and Wilkins Company, Baltimore,M.D.
Cappuccino, J. G and Sherman, N. 1999. Microbiology, A Laboratory Manual. Fourth Ed. Addison-Wesley, Harlow, England, pp.263.
FDA (U. S. Food and Drug Administration), 1986. Aquaculture drug notice by USFDA. Aquacult. Mag., 12 (4) : 4-5.
Felix, S, 2000. Occurrence of pathogenic bacteria in shrimp farming systems of Tamil Nadu. J. Aqua. Trop., 15(4) : 365-369.
Hameed, A. S. S. and Rao, P. V. 1994. Studies on the chemical control of a Vibrio campbelli-like bacterium affecting hatchery reared Penaeus indicus larvae. Aquaculture, 127 : 1-9.
Hameed, A. S. S., Rahaman, K. H., Alagan, A., Yoganandhan, K. 2003. Antibiotic resistance in bacteria isolated from hatchery-reared larvae and post-larvae of Macrobrachium rosenbergii. Aquaculture,
(1/4) : 39-48.
Ho, H. J., Ho, J. M., Heui, C. M., Heon, K. G., Cheon, L. K., Hoon, K. J., Sung, J. T. 2002. The study on fish diseases with reference to bacterial susceptibility to antibiotics in the southern area of Kyeognam. J. Vet. Clinics, 19(1) : 19-22.
Joseph, S. W., Colwell, R. R. and Kaper, J. B. 1982. Vibrio parahaemolyticus and related halophilic vibrios. CRC Crit. Rev. Microbiol, 10 : 77-124.
Karunasagar, I., Pai, R., Malathi, G. R. and Karunasagar, I. 1994. Mass mortality of Penaeus monodon larvae due to antibiotic resistant Vibrio harveyi infection. Aquaculture, 128 : 203-209.
Lavilla-Pitogo, C. R. 1995. Bacterial diseases of penaeid shrimps : an Asian view. pp. 107-121. In M.Shariff, R. P. Subasinghe, and J. R. Arthur (ed), Diseases in Asian Aquaculture II. Fish Health Sec., Asian Fisheries Soc., Manila.
Leano, E. M., Lavilla-Pitogo, C. R., and Paner, M. G. 1998. Bacterial flora in the hepatopancreas of pond reared Penaeus monodon juveniles with luminous vibriosis. Aquaculture, 164 : 367-374.
Lightner, D. V. 1996. A handbook of pathology and diagnostic procedures for diseases of shrimps. Special publication, World Aquacult. Soc. Baton, Rouge, L. A.
Mohney, L. L., Lightner, D. V. and Bell, T. A. 1994. An epizootic of vibriosis in Ecuadorian pond reared Penaeus vannamei Boone (Crustacea : Decapoda). J. World Aquacult. Soc., 25 (1) : 116-125.
Sasmal, D and Abraham, T. J. 2004. Antibiotic sensitivity of bacterial flora associated with diseased prawn Macrobrachium rosenbergii. J. Inland Fish. Soc. India., 36(2) : 68-70.
Sinderman, C. J. and Lightner, D. V. 1988. Disease diagnosis and control in North American Marine Aquaculture, Developments in Aquaculture and Fisheries Science, 2nd ed., Elsevier, Amsterdam.
Thakur, A. B., Vaidya, R. B., Suryawanshi, S. A. 2003. Pathogenicity and antibiotic susceptibility of Vibrio species isolated from moribund shrimps. Indian J. Mar. Sci., 32 (1) : 71-75
Vera, P., Navas, J. I. and Quintero, M. C. 1992. Experimental study of the virulence of three species of Vibrio bacteria in Penaeus japonicus (Bate 1881) juveniles. Aquaculture, 107 : 119-123.
Submitted
Published
Issue
Section
License
In case of publication of the article in the journal, author(s) are required to assign copyright to the Journal of the Inland Fisheries Society of India for its publication in any form/language including all media (print and electronic, or presently unknown), and exclusive right to use the matter for the life of the work (no time restriction on re use of matter).