Optimization of stocking density and its effects on Climbing Perch (Anabas testudineus) cum spinach (Spinacia oleracea) based aquaponic system


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Authors

  • Chinmayee Subhasmita
  • Manoj Kumar Tripathy
  • Saumyendra Nanda
  • Sushanta Kumar Patra
  • Brundaban Sahu
  • Sandeep Shankar Pattanaik
  • Sangram Keshari Rout

https://doi.org/10.47780/jifsi.53.3-4.2021.122362

Abstract

Aquaponic system combines fish culture with hydroponic system for the enrichment of the land value. A small scale aquaponic system was studied for 60 days to investigate fish and plant growth as well as its economic viability using Anabas testudineus as the fish component and spinach as the hydroponic component. Fishes were stocked with three different stocking densities viz., 200 nos. m-³ (T1), 300 nos. m-³ (T2) and 400 nos. m-³ (T3) in triplicates with spinach as plant component in hydroponic beds @ 40 plants/m². In control (T0) A. testudineus were stocked @ 200 nos. m-³with triplicates, where no aquaponics was done. Among various growth parameters weight gain, weight gain percentage, specific growth rate, daily increment, daily growth index, FER and PER of fish were higher in T1 followed by T2, T3 and least was in T0. Fish survival did not vary with high stocking densities. Hence, yield of the fishes was found to be more in T3. Growth parameters of spinach (Spinacia oleracea) such as plant height, height gain, percentage height gain, leaf length, leaf width, plant growth rate and yield during the RESEARCH PAPER experimental period were higher in T3 followed by T2 and T1. Throughout the culture period, highest growth of spinach was recorded in highest fish density, which might be due to availability of more fish waste and nutrients for plants with higher stocking densities of fish. Economic evaluation of the study showed higher benefit cost ratio in T3 (1.53) where fish was stocked @400 nos. m-³ with spinach @40 m-2 followed by T2 (1.49), T1 (1.14) which may be due to the higher stocking density of fish which helps in more yield of both fish and plant. Thus, an aquaponic system with 400 nos. m-3 of A. testudineus in combination with spinach @ 40 nos. m-² was more economically viable for farming practices. Further research is necessary to optimize highest stocking density of both fish and plant to get maximum profit from intensive aquaponic system with sustainable strategies.

Key words Anabas testudineus; Aquaponic system; Spinacia oleracea; stocking density; B:C

References

1. Ahmed A. A. and Hamad A. E. 2013. Effects of stocking

2. density and feeding levels on growth and survival of

3. Nile tilapia (Oreochromis niloticus L.) fry reared in an

4. earthen pond, Khartoum, Sudan. Journal of Science and

5. Technology, 14(2): 95-103.

6. Ali S. 1982. Studies on the diurnal variation in physico-chemical factors and zooplankton in a fresh water pond.

7. Bangladesh Journal of Fisheries, 2(1): 15-23.

8. Anantharaja, K., Mohapatra, B. C., Pillai, B. R., Kumar, R.,

9. Devaraj, C. and Majhi, D. 2017. Growth and survival of

10. climbing perch, Anabas testudineus in Nutrient Film

11. Technique (NFT) aquaponics system. International

12. Journal of Fisheries and Aquatic Studies, 5(4): 24-29.

13. APHA (American Public Health Association), American Water

14. Works Association and Water Pollution Control Federation. 1995. Standard methods for examination of

15. water and wastewater, 13th Washington USA

16. Cerozi da Silva, B. and Fitzsimmons, K. 2016. Use of Bacillus

17. spp. to enhance phosphorus availability and serve as a

18. plant growth promoter in aquaponics systems. Scientia

19. Horticulturae, 211: 277-282.

20. Delaide, B., Goddek, S., Gott, J., Soyeurt, H. and Jijakli, M. H.

21. Lettuce (Lactuca sativa L. var. Sucrine) growth

22. performance in complemented aquaponic solution outperforms hydroponics. Water, 8(10): 467

23. Eissa, I., El-Lamie, M., Hassan, M. and El Sharksy, A. 2015.

24. Impact of aquaponic system on water quality and health

25. status of Nile tilapia Oreochromis niloticus. Suez Canal

26. Veterinary Medical Journal SCVMJ, 20 (2): 191-206.

27. El-Saidy, D. M. and Hussein E. E. S. 2015. Effects of stocking

28. density and water exchange rates on growth performances, production traits, feed utilization and body

29. composition of mono-sex male Nile tilapia,

30. Oreochromis niloticus (L.) cultured in concrete tanks,

31. International Journal of Aquaculture, 5(3): 1-13.

32. FAO 2011. The state of food insecurity in the world. How does

33. international price volatility affect domestic

34. economies and food security, Food and Agricultural

35. Organization, Rome, Italy.

36. Feldlite, M. and Milstein, A., 2000. Effect of density on

37. survival and growth of cyprinid fish fry. Aquaculture

38. International, 7(6): 399-411.

39. Graber, A. and Junge, R. 2009. Aquaponic systems: Nutrient

40. recycling from fish wastewater by vegetable

41. production. Desalination, 246(1-3): 147-156.

42. Hu, Z., Lee, J. W., Chandran, K., Kim, S., Brotto, A. C. and

43. Khanal, S. K. 2015. Effect of plant species on nitrogen

44. recovery in aquaponics. Bioresource technology, 188:

45. -98.

46. Hussain, T., Verma, A. K., Tiwari, V. K., Prakash, C., Rathore,

47. G., Shete, A.P. and Saharan, N. 2015. Effect of water

48. flow rates on growth of Cyprinus carpio var. koi

49. (Cyprinus carpio L., 1758) and spinach plant in

50. aquaponic system. Aquaculture international, 23(1):

51. -384.

52. Jha, P. and Barat. S. 2005. The effect of stocking density on

53. growth, survival rate, and number of marketable fish

54. produced of koi carps, Cyprinus carpio var. Koi in

55. concrete tanks. Journal of Applied Aquaculture, 17(3):

56. -102.

57. Kamble, P.N., Giri, S.P., Mane, R.S. and Tiwana, A. 2015.

58. Estimation of chlorophyll content in young and adult

59. leaves of some selected plants. Universal Journal of

60. Environmental Research and Technology, 5(6):

61. -310.

62. Lawson, T. B. 1995. Recirculating aquaculture systems.

63. Fundamentals of Aquacultural Engineering, Springer,

64. Boston, pp. 192-247.

65. Losordo, T. M., Masser, M. And Rakocy, J. E. 1992.

66. Recirculating aquaculture tank production systems: An

67. overview of critical considerations. SRAC Publications,

68. : 1-5.

69. Love, D. C., Fry, J. P., Li, X., Hill, E. S., Genello, L., Semmens,

70. K. and Thompson, R. E. 2015. Commercial aquaponics

71. production and profitability: Findings from an

72. international survey. Aquaculture, 435: 67-74.

73. Masser, M. P., Rakocy, J. and Losordo, T. M. 1999. Recirculating aquaculture tank production systems: Management

74. of recirculating system. SRAC Publication, p. 452.

75. Mukuve, F. M and Fenner, R. A. 2015. The influence of water,

76. land, energy and soil-nutrient resource interactions on

77. the food system in Uganda. Food Policy, 51: 24-37.

78. Nijhof, M. and Bovendeur, J. 1990. Fixed film nitrification

79. characteristics in sea-water recirculation fish culture

80. systems. Aquaculture, 87(2): 133-143.

81. Papoutsoglou, S. E., Papaparaskeva-Papoutsoglou, E. and

82. Alexis, M. N. 1987. Effect of density on growth rate

83. and production of rainbow trout (Salmo gairdneri) over

84. a full rearing period. Aquaculture, 66(1): 9-17.

85. Patil, P. A., Dube, K., Verma, A. K., Chadha, N. K., Sundaray, J.

86. K. and Jayasankar, P. 2019. Growth performance of

87. goldfish, Carassius auratus and basil, Ocimum basilicum

88. in media bed aquaponics. Indian Journal of Fisheries,

89. : 112-118.

90. Quagrainie, K. K., Flores, R. M. V., Kim, H. J. and McClain, V.

91. Economic analysis of aquaponics and

92. hydroponics production in the US Midwest. Journal of

93. Applied Aquaculture, 30(1): 1-14

94. Rakocy, J. E., Bailey, D. S., Shultz, R. C. and Thoman, E. S.

95. Update on tilapia and vegetable production in

96. the UVI aquaponic system. In: New dimensions on

97. farmed Tilapia: proceedings of the sixth international

98. symposium on Tilapia in Aquaculture, pp. 12-16.

99. Rakocy, J. E., Masser, M. P. and Losordo, T. M. 2006.

100. Recirculating aquaculture tank production systems:

101. Aquaponics-integrating fish and plant culture, SRAC

102. Publication No. 454.

103. Sarkar, U. K., Deepak, P. K., Kapoor, D., Negi, R. S., Paul, S. K.

104. and Singh, S. 2005. Captive breeding of climbing perch

105. Anabas testudineus (Bloch, 1792) with Wova-FH for

106. conservation and aquaculture. Aquaculture Research,

107. (10): 941-945.

108. Shelton, W. L., Smitherman, R. O. and Jensen, G. L. 1981.

109. Density related growth of grass carp, Ctenopharyngodon

110. idella (Val.) in managed small impoundments in

111. Alabama. Journal of Fish Biology, 18(1): 45-51.

112. Shoko, A. P., Limbu, S. M., Mrosso, H. D. J., Mkenda, A. F. and

113. Mgaya, Y. D. 2016. Effect of stocking density on

114. growth, production and economic benefits of mixed sex

115. Nile tilapia (Oreochromis niloticus) and African

116. sharptooth catfish (Clarias gariepinus) in polyculture

117. and monoculture. Aquaculture Research, 47(1): 36-50.

118. Stone, N. M. and Thomforde, H. K. 2004. Understanding your

119. fish pond water analysis report, Cooperative

120. Extension Program, University of Arkansas at Pine

121. Bluff, US Department of Agriculture, pp. 1-4.

122. Swingle, H. S. 1967. Standardization of chemical analysis for

123. waters and pond muds, FAO fish. Rep, 4(44): 397-421.

124. Tripathy, M. K., Sahu, J. P. and Panigrahy, R. C. 2017.

125. Observation on the growth performance of goldfish

126. (Carassius auratus) reared in aquariums with different

127. levels of probiotics. Journal of Experimental Zoology.

128. India, 20(2): 711-716

129. Tripathy, M. K., Sahu, J. P. and Panigrahy, R. C. 2017.

130. Influence of probiotic on the relative condition factor

131. and relative growth co-efficient of Carassius auratus.

132. Journal of Applied Zoological Researches, 28(2):

133. -202

134. Van Rijn, J. and Rivera, G. 1990. Aerobic and anaerobic

135. biofiltration in an aquaculture unit-nitrite accumulation

136. as a result of nitrification and denitrification.

137. Aquacultural engineering, 9(4): 217-234.

138. Zhang, S. Y., Li, G., Wu, H. B., Liu, X. G., Yao, Y. H., Tao, L.

139. and Liu, H. 2011. An integrated recirculating

140. aquaculture system (RAS) for land-based fish farming:

141. The effects on water quality and fish production.

142. Aquacultural Engineering, 45(3): 93-102.

Published

2026-09-01

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Articles

How to Cite

Subhasmita, C., Tripathy, M. K., Nanda, S., Patra, S. K., Sahu, B., Pattanaik, S. S., & Rout, S. K. (2026). Optimization of stocking density and its effects on Climbing Perch (Anabas testudineus) cum spinach (Spinacia oleracea) based aquaponic system. Journal of the Inland Fisheries Society of India, 53(3-4), 143-150. https://doi.org/10.47780/jifsi.53.3-4.2021.122362