DETECTION OF WSSV AND VIBRIO PARAHAEMOLYTICUS IN PENAEUS MONODON (FABRICIUS) USING DNA DOT-BLOT HYBRIDIZATION TECHNIQUE
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Authors
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S S Mishra
CIFRI, Barrackpore
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Manoj P BRAHMANE
Central Inland Fisheries Research Institute, Barrackpore
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Praveen Maurye
Central Inland Fisheries Research Institute, Barrackpore, Kolkata - 700 120
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P Mali
Central Inland Fisheries Research Institute, Barrackpore, Kolkata - 700 120
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Manas Kumar Das
Central Inland Fisheries Research Institute, Barrackpore, Kolkata - 700 120
Abstract
Penaeus monodon, samples collected from different shrimp farms located in North 24 Parganas, West Bengal, were analyzed for detection of White Spot Syndrome Virus (WSSV) and Vibrio parahaemolyticus. Affected shrimp exhibited reddish body discoloration with necrosis of appendages. Minute white spots or patches on carapace and body cuticle were also visible. WSSV and V. parahaemolyticus DNA were amplified using PCR and the PCR amplified products were used as DNA probes. DNA dot blot hybridization using alkaline-phosphatase (AP) labelled DNA probes, specific for WSSV and V. parahaemolyticus detected DNA in tissue samples.
References
Adams, A. 1991. Detection of Vibrio parahaemolyticus biotype alginolyticus in penaeid shrimp using an amplified enzyme immunoassay. Aquaculture, 93 :101- 108.
Azad., I. S., Shekhar, M. S., Mishra, S. S., Santiago, T. C. and Rao, L. H. 2002. Detection of WSV specificity to different tissues of tiger shrimp (Penaeus monodon), from east-coast of India, by polymerase chain reaction and histopathology. J. Aqua. Trop., 17 (3) : 175-184.
Buchanan, R. E. and Gibbons,N. E. 1988. Burgey’s manual of determinative bacteriology, 8th Ed. Williams and Wilkins Company, Baltimore, M.D.
Chang, P. S., Chen, H. C. and Wang,Y. C. 1998. Detection of white spot syndrome associated baculovirus in experimentally infected wild shrimp, crab and lobsters by in situ hybridization. Aquaculture, 164 : 233-242.
Chen, D., Hanna, P. J., Altman, K., Smith, A., Moon, P. and Hammond, L. S. 1992. Development of monoclonal antibodies that identify Vibrio species commonly isolated from infections of humans, fish and shellfish.
Appl. Environ. Micro., 58 : 3694-3700.
Chen, Z. J., Wang, S. S. and Shih, H. H. 2002. An assay for quantification of white spot syndrome virus using a capture ELISA. J. Fish Dis., 25 : 249-251.
Chou, H. Y., Huang, C. Y., Wang, C. H., Chiang, H. C., Lo, C. F., 1995. Pathogenecity of a baculovirus infection causing white spot syndrome in cultured shrimp in Taiwan. Dis. Aquat. Org., 23 : 165-173.
Hameed, A. S. S., Anilkumar, M., Raj, M. L. S. and Jayakumar, K. 1998. Studies on the pathogenicity of systemic ectodermal and mesodermal baculovirus and its detection in shrimp by immunological methods.
Aquaculture, 160 : 31-45.
Hameed, A. S. S. and Rao, P. V., 1994. Studies on the chemical control of a Vibrio campbelli-like bacterium affecting hatchery reared Penaeus indicus larvae. Aquaculture, 127 : 1-9.
Inouye, K., Miwa, S., Oseko, N., Nakano, H., Kimura, T., Momoyama, K. and Hiraoka, M. 1994. Mass mortalities of cultured kuruma shrimp, Penaeus japonicus in Japan in 1993: Electronmicroscopic evidence of the causative virus. Fish Pathol. 29 : 149-158.
Jayasree, J., Janakiram, R. and Madhavi, R. 2000. Characteristics, pathogenecity and antibiotic sensitivity of bacterial isolates from white spot diseased shrimp. Asian Fish. Sci., 13 : 327-334.
Kaper, J. B., Campen, K. R., Seidler, R. J., Baldini, M. H., and Falkow, S. 1984. Cloning of the thermostable direct or kanagawa phenomenon associated hemolysin in Vibrio parahaemolyticus. Infect. Immun. 45 : 290-292.
Karunasagar, I. and Karunasagar, I. 1996. Practical manual, International workshop on Molecular methods for rapid detection of seafood associated pathogens. University of Agril. Sci, Mangalore, India. 57 p.
Karunasagar, I., Otta, S. K. and Karunasagar, I. 1997. Histopathology and bacteriological study of white spot syndrome of Penaeus monodon along the West Coast of India. Aquaculture, 153 : 9-13.
Lavilla-Pitogo, C. R., 1995. Bacterial diseases of penaeid shrimps : an Asian view, pp. 107-121. In Shariff, M., Subasinghe, R. P. and Arthur, J. R. (ed), Diseases in Asian Aquaculture II. Fish Health Sec., Asian
Fisheries Soc., Manila
Lightner, D. V. 1996. A handbook of pathology and diagnostic procedures for diseases of shrimps. Special publication, World Aquacult. Soc. Baton, Rouge, L. A.
Lightner, D. V. and Redman, R. M. 1998. Shrimp disease
and current diagnostic methods. Aquaculture, 164 : 201-220.
Lo. C. F., Leu, J. A., Ho, C. H., Chen, C. H., Peng, S. E., Chen, Y. F., Chou, C. M., Yeh, P. Y., Huang, C. J., Chou, H. Y., Wang, C. H. and Kou, G. H. 1996. Detection of baculovirus associated with white spot syndrome (WSBV) in penaeid shrimp using polymerase chain reaction. Dis. Aquat.Org., 25 : 133-141.
Lu, C. C., Tang, K. F. J., Kou, G. H and Chen, S. N. 1993. Development of a Penaeus monodon-type baculovirus (MBV) DNA probe by polymerase chain reaction and sequence analysis. J. Fish Dis., 16 : 551-559.
Manohar, M. B., Sundaraj, A., Selvaraj, D., Shella, P. R., Chidabaram, P., Mohan, A. C. and Ravisankar, B. 1996. An outbreak of SEMBV and MBV infection in cultured Penaeus monodon in Tamil Nadu, Indian J. Fish. 43 : 403 - 406.
Mishra, S. S. 1998. Use of dot immunoassay for rapid detection of pathogenic bacteria Vibrio alginolyticus and Aeromonas hydrophila from shrimps and fishes. Indian J. Mar. Sci., 27 : 222-226.
Mishra, S. S. 2002. Recent advances in shrimp disease diagnosis, pp 151-157. In Ayyappan, S., Jena, J. K., and Joseph, M. M. (ed), The Fifth Indian Fisheries Forum Proceedings, AFSIB, Mangalore and AoA,
Bhubaneswar, India.
Mishra, S. S. and Shekhar, M. S. 1997. Enzyme-linked immunosorbent assay and dot immunoassay for detection of Vibrio spp. in tiger shrimp, Penaeus monodon. Indian J. Fish., 44(4) : 369-376.
Mishra, S. S., Panigrahi, A. and Mishra, S. K. 2001. Towards development of diagnostic kit based on nitrocellulose enzyme immunoassay for rapid detection of WSSV in shrimp. Fishing Chimes, 21(2) : 19-21.
Mishra, S. S., Shekhar, M. S., Mishra, S. K. and Thiagarajam, V. 2002. Development of gene probe for rapid detection of white spot syndrome virus in shrimp using dot-blot hybridization technique: scope for field
application. Fishing Chimes., 22 : 44-48.
Mialhe, E., Boulo, V., Bachere, E., Hervio, D., Cousin, K., Noel, D., Noel, T., Ohresser, M., Deuff, Le. R. M., Despress, B. and Gendreau, S. 1992. Development of new methodologies for diagnosis of infectious diseases
in molluscs and shrimp. Aquaculture, 107 : 155- 164.
Shekhar, M. S. and Mishra, S. S. 1998. Rapid detection of white spot virus in penaeid shrimp by PCR technique. Fishing Chimes, 18 : 30-31.
Shekhar, M. S., and Mishra, S. S. 2000. Detection of Vibrio parahaemolyticus from shrimp by polymerase chain reaction. pp. 231-233. In Natarajan, P. and Jayaprakas, V. (ed), Advances in Aquaculture, Univ. Kerala, Trivandrum, India.
Takahashi, Y., Itami, T., Kondo. M., Maeda. M., Fuji. R., Tomonga, S., Supamattaya, K., Boonayaratpalin, S.1994. Electronmicroscopic evidence of bacilliform virus infection in kuruma shrimp (Penaeus japonicus). Fish Pathol., 29 : 121-125.
Takahashi, Y., Itami, T., Maeda, M., Suzuki, N., Kasorchandra, J., Supamattaya, K., Khongprudit, R., Boonayaratpalin, S., Kondo, M., Kawai, K., Kasuda, R., Horono, I. and Aoki, T. 1996. Polymerase chain reaction (PCR) amplification of bacilliform virus (RV-PJ) DNA in Penaeus japonicus, Bate and Systemic ectodermal and mesodermal baculovirus (SEMBV) DNA in Penaeus monodon (Fabricious). J. Fish Dis., 19 : 399-403.
Wongteerasupaya, C., Vickers, J. E., Sriurairatana, S., Nash, G. L., Akarajamorn, A., Bonsaeng, V., Panyiem, S., Tassanakajon, A., Withachumnarnkul, B. and Flegel, T. W. 1995. A non-occluded systemic baculovirus that occurs in cells of ectodermal and mesodermal origin
and causes high mortality in the black tiger prawn, Penaeus monodon. Dis. Aquat. Org., 21 : 69 - 77.
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