OCCURRENCE OF NITROGEN FIXING AEROBIC BACTERIA AND AZOTOBACTER SPP. IN A FRESHWATER LAKE AND A POND AT MUMBAI
157 / 9
Abstract
Biological nitrogen fixation is an important process in nitrogen cycle in aquatic environment. In the present study, enumeration of nitrogen-fixing bacteria and effect of physico-chemical parameters of water and soil from Powai lake and Aarey fishpond, Mumbai, on their distribution has been studied. Five species of nitrogen-fixing Azotobacter (Azotobacter chroococcum, A. vinelandii, A. beijerinckii, A. nigricans and A. armeniacus) were characterized from water and soil. Higher number of nitrogen-fixing Azotobacter population could be observed in soil than in water. The nitrogen-fixing capacity of A. chroococcum, isolated from soil was higher than the nitrogen-fixing capacity of A. vinelandii and A. nigricans isolated from water.
References
APHA, 1998. Standard methods for the examination of water and waste water 20th Edn. American Public Health Association, Washington D.C.
Baneljea, S. M. 1967. Water quality and soil condition of fish ponds in some states of India in relation to fish production. Indian J. Fish., 14 : 115-141.
Barat, S. 1986. Microbiological investigation of nitrogen cycle and water in the air breathing catfish farming and polyculture system. Ph. D. thesis, University of Ka1yani, West Bengal, India. 227 p.
Burris, R H. and Wilson, P. W. 1946. Comparison of the metabolism of ammonia and molecular nitrogen in Azotobacter. J. BioI. Chern., 165: 595-598.
Dash, B. 1993. Biological nitrogen fixation in freshwater fishpond ecosystem with reference to hydrobiological condition. Ph. D. thesis, Orissa University of Agriculture and Technology, Bhubaneswar, India.
EI-Samra, M. I. and Olah, 1. 1980. Magnitude and pattern of nitrogen fixation in fishpond. Aquacultura Hungarica (Szarvas), 2: 94-104.
Guerinot, M. L. and Colwell, R. A. 1985. Enumaration, isolation and characterization of nitrogen fixing bacteria from sea water. Appl. Emliron. Microbiol., 50 :350-355.
Jana, B. B. and Roy, S. K. 1986. Seasonal and spatial distribution pattern of nitrogen fixing bacteria in fish ponds under different management system. Hydrobiologia, 137 : 45-54.
Jensen, H. L. 1954. The Azotobacteriaceae. Bact. Rev., 18 (4) : 195-214.
Jensen, V. 1981. Heterotrophic microorganism. pp 30-56. In Broughton W. J. (ed.), Nitrogen fixation, Ecology, Vol. 1, Clarendon Press, Oxford.
Kawai, A. and Sugahara, 1. 1971. Microbiological studies on nitrogen fixation in aquatic environment 1. On the distribution of niirogen fixing bacieria in freshwaier region. Buli. Jap. Soc. Sci. Fish., 37 : 740-746.
Kawai, A., Sugiyama, M. and Sugahara, 1. 1971.
Microbiological studies on nitrogen fixation in aquatic environment IV. Bull. Jap. Soc. Sci. Fish., 37: 986-991.
Krieg, N. R. and Holt, L. G. 1984. Bergey’s Manual of Systematic Bacteriology. Vol. 1. Lippincott Williams and Wilkins, Baltimore. pp 219-233.
Paerl, H. W., Crocker, K. W. and Profert, L. E. 1987.
Limitation of nitrogen fixation in coastal marine waters: relative importance of molybdenum, iron, phosphorous and organic matter availability. Limnol. Oceanogr., 32 : 525-536.
Pandey, P. K. 1997. Nitrogenous biofertilization with Azolla in freshwater fishpond ecosystem. Ph.D. thesis, Central Institute of Fisheries Education, Mumbai, India. 456 p.
Rao, N. S. 1979. Recent advances in biological nitrogen fixation. Oxford and IBH Publishing Company, New Delhi, India. 486 p.
Snedecor, G. W. and Cochran, W. G. 1989. Statistical methods. 8th ed., Iowa State University Press, Ames, Iowa, U.S. A. 505 p.
Stewart, W. D. P., Fitzgerald, G. P. and Burris, R H. 1978. Acetylene reduction by nitrogen fixing blue green algae. Arch. Microbiol., 62: 336-348.
Sugahara, L. and Kawai, A. 1971. Microbiological studies on nitrogen fixation in aquatic environment V. Modification of acetylene method for the measurement of in situ ratio of nitrogen fixation. Bull. Jap. Soc. Sci. Fish., 37: 1988-1992.
Terzaghi, B. E. and Terzaghi, E. 1986. Azotobacter biology, biochemistry and molecular biology, pp. 127-141. In Broughton, W. J. and Phuler, A. (eds.), Nitrogen Fixation, Vol 4, Molecular Biology, Claredon Press,Oxford. Thompson, J. P. and Skerman, V. B. D. 1979.
Azotobacteriaceae : The Taxonomy and Ecology of Aerobic Nitrogen Fixing Bacteria. Academic Press, London. 417 p.
Tripathy, P. P. and Ayyappan, S. 2006. Evaluation of Azotobacter sp. and. Azospirillum sp. as biofertilizer in freshwater fishpond ecosystems. World J. Microbiol. Biotechnol. 21 (8-9): 1339-1343.
Downloads
Submitted
Published
Issue
Section
License
In case of publication of the article in the journal, author(s) are required to assign copyright to the Journal of the Inland Fisheries Society of India for its publication in any form/language including all media (print and electronic, or presently unknown), and exclusive right to use the matter for the life of the work (no time restriction on re use of matter).