Human IgG-crossreactive sarcoplasmic peptides in the freshwater catfish Rita rita
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Authors
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Santosh Kumar Gupta
-
Sasmita Mohanty
-
Arabinda Mahanty
-
Bimal Prasanna Mohanty
Head of Division (FREM)
Principal Scientist
ICAR-CIFRI, Barrackpore
Abstract
The prevalence and magnitude of clinical
allergy caused by crossreacting proteins and
panallergens appears to be increasing and reflects an
increase in allergen sensitization. Fish is reported to be
one of the most common causes of food allergic
reactions. In general, clinically manifested food allergy
is regarded as IgE mediated. However, there are some
cases (~ 10%) in which IgE can not be proved. The
present investigation was undertaken to identify the
IgG-cross-reactive sarcoplasmic peptides in the Indian
freshwater catfish Rita rita and to study the effect of
boiling/ processing on their immunoreactivity. The
epaxial (white) muscle proteins were extracted in PBS
under three different conditions, viz. 'raw' (or uncooked,
UC), 'boiled' (or cooked, C) and 'cooked with spices' as
a normal ethnic preparation (CS). The protein profiles
were analyzed by SDS PAGE and 2-D PAGE. Titer of
human IgG-crossreactive sarcoplasmic peptides was
determined by ELISA and cross-reactive proteins/
peptides were identified by immunoblot. By silver
staining the SDS-polyacrylamide gel, ~23 protein bands
were visualized in the protein extract (UC). Four
'thermostable' or 'boiling resistant' bands, viz. 55, 45, 23,
& 20 kDa, were observed of which, the 23 kDa
(pI 5.5-5.9), followed by 45kDa (pI 5.1-5.5) were the major
ones. Muscle protein-specific IgG titers in the human
sera, from a randomly selected population (n = 18), that
included both fish-eating and fish-non-eating
individuals varied from 1/1000 to 1/8000. Variable
numbers of immunoreactive proteins were observed with different sera samples on immunoblot. In 'C' and 'CS' samples also immunoreactive bands were seen. There are cross-reactive proteins among the fish
sarcoplasmic peptides that are recognized by human IgG and some of them are 'boiling resistant' and retain the immunoreactivity even after boiling and complex processing reactions that might account, partly, for the non-IgE-mediated food allergy. We could identify a 41kDa boiling-resistant sarcoplasmic peptide (pI 5.9-6.6), which appears to be a major IgG-crossreactive peptide in the freshwater catfish Rita rita.
Keywords IgG-crossreactive, peptides, Fish food, allergy, hypersensitivity, catfish
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