Characterization of F1 hybrids developed from the crosses between Brassica rapa and Brassica fruticulosa: A step towards the development of synthetic transient amphidiploids

Abstract

 In the present study, the F₁ hybrids were developed from the crosses between Brassica rapa and Brassica fruticulosa  through wide hybridization. Morphological, molecular, cytological and fertility analyses were done to identify the true 
hybrids. Further amphidiplodization can be done for the development of synthetic amphidiploid. Most of the F 1
 progenies were sterile when analyzed for pollen viability, and the pollen was not globular. The mitotic analysis of 
hybrids showed 2n=18 chromosomes.  MS-70 SSR primer was identied as the polymorphic marker among the parents 
from a set of fifty-three SSR primers used for hybridity confirmation. Also, the morphometric characters of the F₁ hybrids 
 were analyzed, and some showed the intermediate plant type from both the parents, viz. type of petiole, leaf edge, leaf 
waxiness, leaf colour, leaf hairiness, flower colour, and the anther type in the flowers are rudimentary which are visible 
very easily. On the basis of these analyses total of 34 F₁ plants out of 35 plants were identied as true hybrids.  These true hybrids will be further amphidiplodized using colchicine and used for the alien introgression line development program.