Marker assisted selection for developing superior breeding lines for white rust resistance in Indian mustard (Brassica juncea) using IP markers


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Authors

  • Disha Kamboj Department of Molecular Biology, Biotechnology and Bioinformatics, Chaudhary Charan Singh Haryana Agricultural University, Hisar-125004, Haryana, India Author
  • Neelam R Yadav Department of Molecular Biology, Biotechnology and Bioinformatics, Chaudhary Charan Singh Haryana Agricultural University, Hisar-125004, Haryana, India Author
  • Neelam R Yadav Department of Molecular Biology, Biotechnology and Bioinformatics, Chaudhary Charan Singh Haryana Agricultural University, Hisar-125004, Haryana, India Author
  • Ram Avtar Department of Genetics and Plant Breeding, Chaudhary Charan Singh Haryana Agricultural University, Hisar-125005, Haryana, India Author

https://doi.org/10.56093/job.v14i2.

Keywords:

CAPS, Indian mustard, marker assisted selection, IP, simple sequence repeat

Abstract

White rust caused by Albugo candida is one of the significant diseases for yield reduction in rapeseed-mustard.
Molecular marker technology in combination with the traditional breeding method offers precise selection in crop
improvement programme. RH0749 a commercial and susceptible variety of Indian mustard was crossed with resistant
donors (Bio-YSR and BEC-144) for improving white rust resistance. BC1F1, BC2F1 populations were evaluated for various
yield attributes and analysed at molecular level to undertake marker-assisted selection (MAS). Intron polymorphism and
cleaved amplified polymorphic sequence markers were used for forward selection. Three IP markers in RH0749 × Bio-YSR
cross and two IP markers in RH0749 × BEC-144 cross showed polymorphism. Three hundred and forty-eight SSRs were
used for background selection and 72 SSR markers were found polymorphic in RH0749 × Bio-YSR cross while in RH0749
× BEC-144 cross, only 43 SSR markers were found polymorphic. The promising progenies (9 in BC2F1 for RH0749 × Bio-
YSR and 8 in BC2F1 for RH0749 × BEC-144 cross) were selected based on molecular, morphological and disease data
which can be further used in Indian mustard improvement programme for white rust resistance.

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Submitted

2024-10-10

Published

2026-04-09

Issue

Vol. 14 No. 2 (2023)

Section

Articles

How to Cite

Marker assisted selection for developing superior breeding lines for white rust resistance in Indian mustard (Brassica juncea) using IP markers (Disha Kamboj, Neelam R Yadav, Neelam R Yadav, & Ram Avtar, Trans.). (2026). Journal of Oilseed Brassica, 14(2), 135-141. https://doi.org/10.56093/job.v14i2.