Determination of mustard seeds endophytic bacterial population and species composition by development of new method
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Authors
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Tamali Sinha
Institute of Advanced Study in Science and Technology, Guwahati- 781035, Assam, India
Author
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Narayan C Talukdar
Institute of Advanced Study in Science and Technology, Guwahati- 781035, Assam, India
Author
Keywords:
Bacteria, diversity, endophytes, mustard, oilseed, 16S rRNA sequencingAbstract
Endophytic bacteria (EB) population and species diversity in seed of oilseeds crops remain unexplored. This study was
conducted to overcome the difficulty imposed in serial dilution method employed for determination of mustard seed EB.
The EB population in centrifuged pellet of per gram surface sterilized seeds (SSS) of 4 mustard varieties ranged from 4.11-
5.16 log Cfu. Addition of known concentrations (20-100 μl) of surfactant (Tween 20) to crushed SSS and water suspension
removed the problem of colony development on nutrient agar and by this method EB population ranged 3.08- 3.37 Cfu/
g seed. The method successfully quantified seed EB of peanut (3.60 log Cfu/g) and sunflower (3.93 log Cfu/g). 16S rRNA
based identification showed that Bacillus species of phyla Firmicutes were abundant in mustard and will facilitate further
studies on seed EB role in mustard crop. Overall, our study provides knowledge and information on bacterial diversity
in mature mustard seeds.
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