Heat stress induced changes in protein profile of Indian mustard (Brassica juncea L.)
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Authors
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Babita Rani
Department of Chemistry and Biochemistry, 1Department of Genetics and Plant Breeding, CCS Haryana Agricultural University Hisar, 125004 India
Author
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Nisha Kumari
Department of Chemistry and Biochemistry, 1Department of Genetics and Plant Breeding, CCS Haryana Agricultural University Hisar, 125004 India
Author
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Veena Jain
Department of Chemistry and Biochemistry, 1Department of Genetics and Plant Breeding, CCS Haryana Agricultural University Hisar, 125004 India
Author
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Kamal Dhawan
Department of Chemistry and Biochemistry, 1Department of Genetics and Plant Breeding, CCS Haryana Agricultural University Hisar, 125004 India
Author
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Ram Avtar
Department of Chemistry and Biochemistry, 1Department of Genetics and Plant Breeding, CCS Haryana Agricultural University Hisar, 125004 India
Author
Keywords:
Heat shock proteins, heat stress, Indian mustard, SDS-PAGEAbstract
The present investigation was undertaken to study the changes in protein pattern of seedlings of Indian
mustard (Brassica juncea L.) after subjecting to high temperature stress (45±0.5°C) and on revival. Two
thermo-tolerant genotypes including BPR-542-6 and NRCDR-02 and two thermo-susceptible genotypes
including NPJ-119 and RGN-152 were screened at 45±0.5°C on the basis of time taken to 50% seedling
mortality and protein pattern of the seedlings was determined by SDS-PAGE. A major band of 53.12 kDa and
minor bands of 100, 89.12, 74.13, 46.76 and 38.9 kDa in thermo-tolerant genotypes and protein bands of
molecular weight 25.79 and 30.7 kDa in thermo-susceptible genotypes appeared under high temperature
stress which disappeared when the stress was relieved. The newly synthesized proteins might be stress
related proteins (heat shock proteins) which may play active role in imparting thermo-tolerance.
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