Genome editing without the hassles of tissue culture: Hope for editing plants recalcitrant to in vitro manipulations
GENOME EDITING : HOPE FOR EDITING PLANTS RECALCITRANT TO IN VITRO MANIPULATIONS
104 / 3
Authors
-
PAPA RAO VAIKUNTAPU
1 ICAR-Indian Institute of Oilseeds Research, Rajendranagar, Hyderabad-500 030, Telangana 2 ICAR-Directorate of Groundnut Research, Junagadh-362 001, Gujarat
-
V DINESH KUMAR
1 ICAR-Indian Institute of Oilseeds Research, Rajendranagar, Hyderabad-500 030, Telangana 2 ICAR-Directorate of Groundnut Research, Junagadh-362 001, Gujarat
-
M SUJATHA
1 ICAR-Indian Institute of Oilseeds Research, Rajendranagar, Hyderabad-500 030, Telangana 2 ICAR-Directorate of Groundnut Research, Junagadh-362 001, Gujarat
-
T RADHAKRISHNAN
1 ICAR-Indian Institute of Oilseeds Research, Rajendranagar, Hyderabad-500 030, Telangana 2 ICAR-Directorate of Groundnut Research, Junagadh-362 001, Gujarat
Keywords:
Biotic/abiotic stress, CRISPR/Cas9, Genome editing, Mobile sgRNA, Oilseed crops, Plant tissue cultureAbstract
Genome editing has become a breakthrough technology this decade to preciselymodify the genome in short time. This technology has paved way for manipulating the traits of agronomic importance with ease in many crop plants. Even though plant transformation is necessary in the first step, the final product is non-transgenic; thus, attractive for social acceptance. Lack of robust transformation procedures is a limitation to use the genome editing in some crop plants. It becomes even harder when the frequency of precise editing effected by the reagents is low. Recent reports show that genome editing can be performed bypassing the tissue culture procedures; therefore, offer hope for its use in crops that are unresponsive. We briefly discuss these breakthrough techniques here.
Downloads
References
Ali Z, Ayman E, Shakila A, and Magdy M M 2018. Pea early-browning virus-mediated genome editing via the CRISPR/Cas9 system in Nicotiana benthamiana and Arabidopsis. Virus Research, 244: 333-37. DOI: https://doi.org/10.1016/j.virusres.2017.10.009
Altpeter F et al 2016. Advancing crop transformation in the era of genome editing. Plant Cell, 28(7): 1510-20.
Clough S J and Andrew F B 1998. Floral Dip: A simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana. The Plant Journal, 16(6): 735-43. DOI: https://doi.org/10.1046/j.1365-313x.1998.00343.x
Cody W B, Herman B S, and Mirkov T E 2017. Multiplexed gene editing and protein overexpression using a tobacco mosaic virus viral vector. Plant Physiology, 175(1): 23-35. DOI: https://doi.org/10.1104/pp.17.00411
Ellison E E, Nagalakshmi U, Gamo M E, Huang P, Dinesh-Kumar S, Voytas D F 2020. Multiplexed heritable gene editing using RNA viruses and mobile single guide RNAs. Nature Plants, 6(6): 620-624. DOI: https://doi.org/10.1038/s41477-020-0670-y
Gao Q, Xu W, Yan T, Fang X, Cao Q, Zhang Z, Ding Z, Wang Y, Wang X 2019. Rescue of a plant cytorhabdovirus as versatile expression platforms for planthopper and cereal genomic studies. New Phytologist, 223(4): 2120-33. DOI: https://doi.org/10.1111/nph.15889
Jinek M, Chylinski K, Fonfara I, Hauer M, Doudna J A, Charpentier E 2012. A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity. Science, 337(6096): 816-821. DOI: https://doi.org/10.1126/science.1225829
Lowe K et al. 2016. Morphogenic regulators baby boom and wuschel improve monocot transformation. The Plant Cell, 28(9): 1998-2015. DOI: https://doi.org/10.1105/tpc.16.00124
Maher M F, Nasti R A, Vollbrecht M, Starker C G, Clark M D, Voytas D F 2020. Plant gene editing through de novo induction of meristems. Nature Biotechnology, 38(1): 84-89. DOI: https://doi.org/10.1038/s41587-019-0337-2
Manghwar H, Keith L, Xianlong Z, and Shuangxia J 2019. CRISPR/Cas system: Recent advances and future prospects for genome editing. Trends in Plant Science, 24(12): 1102-25. DOI: https://doi.org/10.1016/j.tplants.2019.09.006
Nelson-Vasilchik K, Hague J, Mookkan M, Zhang Z J, Kausch A 2018. Transformation of recalcitrant sorghum varieties facilitated by Baby Boom and Wuschel2. Current Protocols in Plant Biology, 3(4): e20076. DOI: https://doi.org/10.1002/cppb.20076
Notaguchi M, Tetsuya H, and Takamasa S 2015. Identification of mRNAs that move over long distances using an RNA-seq analysis of Arabidopsis/Nicotiana benthamiana heterografts. Plant and Cell Physiology, 56(2): 311-321. DOI: https://doi.org/10.1093/pcp/pcu210
Subedi U, Ozga J A, Chen G, Foroud N A, Singer S D 2020. CRISPR/Cas-mediated genome editing for the improvement of oilseed crop productivity. Critical Reviews in Plant Sciences, 39(3): 195-221. DOI: https://doi.org/10.1080/07352689.2020.1782568
Zhang D et al. 2020. Genome editing with the CRISPR?Cas System: An art, ethics and global regulatory perspective. Plant Biotechnology Journal, 18(8): 1651-1669. DOI: https://doi.org/10.1111/pbi.13383
Zhu H, Chao L and Caixia G 2020. Applications of CRISPR-Cas in agriculture and plant biotechnology. Nature Reviews Molecular Cell Biology, http://www.nature.com/articles/ s41580-020-00288-9.
