Isolation of lignocellulolytic bacteria from oil palm mesocarp waste
ISOLATION OF LIGNOCELLULOLYTIC BACTERIA FROM OIL PALM MESOCARP WASTE
84 / 2
Authors
-
P VEERA LAKSHMI
Sri Parasakthi College for Women, Manonmaniam Sundaranar University, Courtallam-627 802, Tamil Nadu
-
K PARANI
Sri Parasakthi College for Women, Manonmaniam Sundaranar University, Courtallam-627 802, Tamil Nadu
Keywords:
Bacterial isolates, Laccase, Lignocellulolytic index, Oil palm mesocarp wasteAbstract
In the present investigation, the bacterial strains were isolated from oil palm mesocarp waste dumped soil by serial dilution and pour plate method. The highest lignocellulolytic index was produced by bacterial isolate OPMW 6 (1.5 mm) followed by OPMW 5 (1.2 mm), OPMW 1 (0.83 mm), OPMW3 and OPMW 4 (0.71 mm). Changes in methylene blue color supplemented in nutrient broth from blue to light green or yellow were observed by three bacterial isolates (OPMW1, OPMW 3 and OPMW5), and rest of the bacterial isolates recorded less decolourization as the cultures were incubated between 24 and 48 hours respectively with highest percentage of dye decolourization produced by bacterial isolate i.e. OPMW5 (83.3%) and lowest rate of decolourization by bacterial isolate OPMW 6 (13.54%) as inoculated in nutrient broth supplemented with methylene blue dye. Utilization of gallic acid as supplemented in minimal salt media recorded positive reaction
for 4 bacterial isolates (OPMW 2, OPMW 5, OPMW 6 and OPMW 8) by the formation of dark colouration and addition ofa-naphthol in MSM inoculated broth culturesshowed formation of purple blue colour due to production of laccase activity. Among the 4 best isolates having the highest indices, two isolates of bacteria (OPMW5 and OPMW6) had the best lignocellulolytic activity. Based on enzyme biosynthesis ability, the OPMW5 and OPMW6 were selected as the best isolates as it can synthesie cellulase and ligninase enzymes. These enzymes are known to accelerate the degradation of oil palm mesocarp fibre substrate.
Downloads
References
Ariana C K P 2017. Isolation and characterization of lignocellulolytic microbes from oil palm empty fruit bunches (EFB). Journal of Environment and Sustainability, 1(1): 1-9.
Atlas R M and Bartha R 1986. Microbial Ecology: Fundamentals and Applications, Menlo Park:Benjamin-Cummings Pub. Co.
Bholay A D 2012. Bacterial lignin peroxidise: A tool for biobleaching and biodegradation of industrial effluents. Journal of Environment Resource Technology, 2(1): 58-64.
Chen Y, Chai L, Tang C, Yang Z, Zheng Y, Shi Y and Zhang H 2012. Kraft lignin biodegradation by Novosphingobium sp. B-7 and analysis of the degradation process. Bioresource Technology, 123: 682.
Gusma W 1999. Pengaruh pemberian mikroorganisme selulolitik dan kotoran ayam terhadap dekomposisi tandan kosong kelapa sawit, Universitas Sumatera Utara.
Kumar P, Barret D M, Delwiche M J and Stroeve P 2009. Methods for pretreatment of lignocellulosic biomass for efficient hydrolysis and biofuel production. Industrial and Engineering Chemistry Research, 48(8): 3713-3729.
Pointing S B 1999. Qualitative methods for the determination of lignocellulolytic enzyme production by tropical fungi. Fungal Diversity, 2.
Rahman S H A, Choudhury J P, Ahmad A L and Kamaruddin A H 2006. Optimization studies on acid hydrolysis of oil palm empty fruit bunch fiber for production of xylose. Bioresource Technology, 98(3): 554-559.
Sulaiman F and Abdullah N 2011. Optimization conditions for maximising pyrolysis liquids of oil palm empty fruit bunches. Energy, 36: 2352-2359.
Suriadikarta D A, Simanungkalit R D M, Saraswati R, Setyorini D and Hartatik W2006. Pupuk organik dan pupk hayati.bogor: Balai besar litbang sumbar daya lahan pertanian.
Thormann M N, Currah R S and Bayley S E 2002. The relative ability of fungi from Sphagnam fuscum to decompose selected carbon substrates. Canadian Journal of Microbiology, 48(3): 204-211.
Wahyuni M 2008. Laju Dekomposisi Aerob dan Mutu Kompos Tandan Kelapa Sawit dengan Penambahan Mikroorganisme Selulolitik, Amandemen dan Limbah Cair Pabrik Kelapa Sawit, Universitas Sumatera Utara.
Zverlov V V, Holl W and Schwarz W H 2003. Enzymes for digestion of cellulose and other polysaccharides in the gut of longhorn beetle larvae, Rhangium inquister L., (Coleoptera: Cerambycidae). International Biodeterioration and Biodegration, 51(3): 175-179.
