Interspecific hybrid between silver leaf sunflower (Helianthus argophyllus T. and G.) and cultivated sunflower: Cytomorphological characterization of F1 hybrid
INTERSPECIFIC HYBRIDIZATION BETWEEN SILVER LEAF SUNFLOWER AND CULTIVATED SUNFLOWER
93 / 1
Authors
-
H P MEENA
ICAR-Indian Institute of Oilseeds Research, Rajendranagar, Hyderabad-500 030, Telangana
-
H D PUSHPA
ICAR-Indian Institute of Oilseeds Research, Rajendranagar, Hyderabad-500 030, Telangana
-
M SUJATHA
ICAR-Indian Institute of Oilseeds Research, Rajendranagar, Hyderabad-500 030, Telangana
Keywords:
Cytomorphological characterization, Helianthus argophyllus, Interspecific Hybridization, SunflowerAbstract
Hybrid plants were obtained by crossing cultivated sunflower (Helianthus annuus L., 2n=2x=34) line ARM-243B and a wild Helianthus species [H. argophyllus; 2n=2x=34; HEL-153/83 (PI-649865)], using the latter as pollen parent. The wild Helianthus accession was selected for this study because of its short duration and short plant height compared to other accessions of H. argophyllus. Morphological and cytological analyses were carried out to confirm the hybrid nature of the F1 plants. The hybrids exhibited morphological features intermediate to both the parents for few attributes and more related to wild Helianthus species like leaf and stem pubescence, stem hairiness, flower colour, stem size, branching, disc floret pigmentation, plant height, seed size and seed shape etc. A reduction in pollen fertility (87.5%) was recorded in F1 plants as compared to both the parents. Meiotic analysis revealed a mixture of univalents, bivalents, trivalents and quadrivalents in all the pollen mother cells (PMCs) analysed. In addition to bivalents and univalents, a trivalent was also observed in few PMCs, indicating segmental homology between chromosomes. Frequently observed chromosome configurations in diakinensis were 15 II + 1 IV and 13 II + 2 IV. The results suggested that the species H. argophyllus and H. annuus differ by 1-2 translocations and 1-2 inversions. Results show that the wild species is compatible with cultivated sunflower and using H. argophyllus cultivated sunflower can be improved for biotic (downy mildew) and abiotic stresses (drought and salinity).
Downloads
References
Alexander P 1969. Differential staining of aborted and non-aborted pollen. Stain Technology, 44: 117-122.
Bohorova H 1983. Tzitogenetichno prouchvane na mejduvidovi hibridi H. scaberimus (2n=68) x H. annuus (2n=34). Otdalechena hibridizatsia pri slanchogleda. C. BAN, pp. 219-228.
Chandler J M, Jan C C and Beard B H 1986. Chromosome differentiation among the annual Helianthus species. Systematic Botany, 11: 354-371.
Chepurnaya A L, Sherstyuk S V, and Tikhomirov V T 2003. CMS-Rf system for sunflower breeding. Helia, 26:59-65.
Christov M 1988. Hybridization between H. argophyllus T. & G. (2n=34) and H. annuus L (2n=34). Genetics and Breeding, 21(6):478-484.
Dussle CM, Hahn V, Knapp S J and Bauer E 2004. PlArg from Helianthus argophyllus is unlinked to other known downy mildew resistance genes in sunflower. Theoretical and Applied Genetics, 109:1083-1086.
Encheva J and Christov M 2006. Interspecific hybrids between cultural sunflower Helianthus annuus L. and Helianthus salicifoliusL. Morphological and biochemical characterization. Helia, 29(45): 107-116.
Espinasse A, Foueillasar J and Kimber G 1995. Cytogenetical analysis of hybrids between sunflower and four wild relatives. Euphytica, 82: 65-72.
Georgieva-Todorova J 1976. Megduvidovi otnoshenia v roda Helianthus L. Sofia. In Bulgarian.
Georgieva Todorova, J 1984. Interspecific hybridization in the genus Helianthus L. Pflanzenz, 93:265-279.
Georgieva-Todorova J 1990. Genetic and cytogenetic investigation of the genus Helianthus L. (in Bulgarian). Bulgarian Academy of Science, Sofia.
Heiser Jr C B 1951. Hybridization in the annual sunflowers: Helianthus annuus × H. argophyllus. American Naturalist, 85:65-72.
Horn R, Kusterer B, Lazarescu E, Prüfe M, Ozdemir N and Friedt W 2002. Molecular diversity of CMS sources and fertility restoration in the genus Helianthus. Helia, 25(36): 29-40.
Hristova and Cherbadzi M M 2004. Hybridization of cultural sunflower Helianthus annuus L. with the annual species Helianthus bolanderi Gray, Helianthus neglectus Heiser and Helianthus petiolaris Nutt all. In: Proc. 16th Intl. Sunflower Conf., Fargo, ND, USA, pp. 699-707.
Jan C C and Gulya T J 2006. Registration of a sunflower germplasm resistant to rust, downy mildew, and virus. Crop Science, 46:1829.
Jan C C, Seiler G J, Gulya T J and Feng J 2008. Sunflower germplasm development utilizing wild Helianthus species. Proc. of 17th Intl. Sunflower Conf., Cordoba, Spain, pp. 29-45.
Korell M, Brahm L, Friedt W and Horn R 1996. Interspecific and intergeneric hybridization in sunflower breeding. II. General breeding aspects. Plant Breeding Abstract, 66: 925-931.
Kulshreshtha V B and Gupta P K 1981. Cytogenetic studies in the genus Helianthus L.II. Karyological studies in twelve species. Cytologia, 46:279-289.
Mallik M, Manivannan N and Chandirakala R 2016. Genetic diversity in sunflower (Helianthus annuus L.). Journal of Oilseeds Research, 33(4) : 243-249.
Manjula T and Seetharam A 2000. Use of wild species Helianthus argophyllus in sunflower improvement. Karnataka Journal of Agricultural Sciences, 13(3): 557-563.
Meena H P, Prashant Kumar Soni and Sujatha M 2016a. Characterization of F1 interspecific hybrids between cultivated sunflower and Helianthus argophyllus T. & G. In: Proceedings of National Seminar on 'Breeding of Field Cropsfor Biotic and Abiotic Stresses in Relation to Climate Change' from 28-29 March, 2016, College of Agriculture, VNMKV, Parbhani. Pp. 42.
Meena H P, Sujatha M, Pushpa HD and Varaprasad KS 2016b. Broadening the genetic base of cultivated sunflower (Helianthus annuus L.) in India through prebreeding. In: Proceedings of 19th International Sunflower Conf., Edirne, Turkey, 2016, pp. 504.
Meena H P, Sujatha M, Prashant Kumar Soni, Pushpa H D and Varaprasad K S 2016c. Cytomorphological and molecular characterization of interspecific hybrids between Helianthus annuus and H. argophyllus T. & G. In: Proceeding of 1st International Biodiversity Congress, New Delhi, India, 2016, pp. 165.
Miller J F and Gulya T J 1988. Registration of six downy mildew resistant sunflower germplasm lines. Crop Science, 28:1040-1041.
Miller J F and Gulya T J 1991. Inheritance of resistance to race 4 of downy mildew derived from interspecific crosses in sunflower. Crop science, 31:40-43.
Miller J F, Gulya T J and Seiler G J 2002. Registration of five fertility restorer sunflower germplasms. Crop Science, 42:989-991.
Narkhede M N, Dharmraj V and Meshram L D 1986. Meiotic studiesin interspecific hybrids ofHelianthus. PanjabraoKrishi Vidyapeeth Research Journal, 10: 84-87.
Nikolova L and Christov M 2004. Interspecific hybridization between Helianthus argophyllus and H. annuus. II. Characteristics of some morphological and phytopathological traits in second and further hybrid generations. Proc. 16 th Intl. Sunflower Conf., Fargo, ND USA, pp. 731-736.
Quagliaro G, Vischi M, Tyrka M and Olivieri A M 2001. Identification of wild and cultivated sunflower for breeding purposes by AFLP markers. Journal of Heredity, 92(1): 38-42.
Quresh Z, Jan C C and Gulya T J 1993. Resistance to sunflower rust and its inheritance in wild sunflower species. Plant Breeding, 110:297-306.
Radwan O, Bouzidi M F, Nicolas P and Mouzeyar S 2004. Development of PCR markers for the Pl5/Pl8 locus for resistance to Plasmopara halstedii in sunflower, Helianthus annuus L. from complete CC-NBS-LRR sequences. Theoretical and Applied Genetics, 109:176-185.
Rauf S 2008. Breeding sunflower (Helianthus annuus L.) for drought tolerance. Communications in Biometry and Crop Science, 3(1): 29-44.
Rogers C E, and Thompson T E 1980. Helianthus resistance to the sunflower beetle (Coleoptera: Chrysomelidae). Journal of the Kansas Entomological Society, 53(4): 727-730.
Rogers C E, Gershenzon J, Ohno N, Mabry T J, Stipanovic R D and Kreitner G L 1987. Terpenes of wild sunflowers (Helianthus): an effective mechanismagainst seed predation by larvae of the sunflower moth, Homoeosoma electellum (Lepidoptera: Pyralidae). Environmental Entomology, 16:586-592.
Saciperov F 1916. Opit skreshchivania dvuh form podsolnechnika H. annuus x H.argophyllus. Tr. Prikl. Bot. I Selekcii, 9(3):18-26.
Schilling E E and Heiser C B 1981. Intrageneric classification of Helianthus (Compositae). Taxon, 30:393-403.
Seiler G J 1991. Registration of 12 downy mildew tolerant interspecific sunflower germplasm lines derived from wild annual species. Crop Science, 31:1714-1716.
Seiler G J 1992. Utilization of wild sunflower species for the improvement of cultivated sunflower. Field Crops Research, 30:195-230.
Seiler G J, Gulya T J and Marek L F 2007. Re-collection of Helianthus argophyllus, source of the P1Arg gene for downy mildew resistance, surviving for 25 years on Daytona Beach, Florida.
Slabaugh M B, Yu J K, Tang S, Heesacker A, Hu X, Lu G, Bidney D, Han F and Knappn S J 2003. Haplotyping and mapping a large cluster of downy mildew resistance gene candidates in sunflower using multilocus intron fragment length polymorphisms. Plant Biotechnology Journal, 1:167-185.
Smith 1991. Flora Vitiensis Nova: A New Flora of Fiji. Vol. 5. Pacific Tropical Botanical Garden.
Sujatha M and Lakshminarayana M 2007. Resistance to Spodoptera litura (Fabr.) in Helianthus species and backcross derived inbred lines from crosses involving diploid species. Euphytica, 155:205-213.
Tavoljanski N, Yesaev A, Yakutkin V, Akhtulova E and Tikhomirov V 2002. Using the collection of wild species in sunflower breeding. Helia, 25:65-77.
Valkova D and Christov M 2004. Characterization of F1 plants obtained from crosses between cultivated sunflower and wild annual Helianthus annuus. In: Proceedings of 16th Intl. Sunflower Conf., Fargo, ND, USA, Pp 747-750.
Velasco L, Pérez Vich B, Jan C C and Fernández-Martínez J M 2007. Inheritance of resistance to broomrape (Orobanche Cumana Wallr.) race F in a sunflower line derived from wild sunflower species. Plant Breeding, 126:67-71.
Vishnu J E, Goud I S, Prabakaran A J, Kulkarni Vand Govindappa M R 2015. Studies on cross-compatibility and characterization of interspecific hybrid between cultivated and wild diploid sunflower. M.Sc. Thesis, University of Agricultural Sciences, Raichur, Karnataka, India.
Whelan E D P 1978. Hybridization between annual and perennial diploid species of Helianthus. Canadian Journal of Genetics and Cytology, 20: 523-530.
Whelan E D P and Dorrell D G 1980. Interspecific hybrids between Helianthus maximiliani Schrad. and H. annuus L. Effect of backcrossing on meiosis, anther morphology and seed characteristics. Crop Science, 20:29-34.
