Characterization of diverse sesame (Sesamum indicum L.) panel through morphological and molecular marker analysis
CHARACTERIZATION OF SESAME PANEL THROUGH MORPHOLOGICAL AND MOLECULAR MARKER
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Authors
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VIVEK KUMAR
Bihar Agricultural University, Sabour, Bhagalpur-813 210, Bihar
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SIMA SINHA
Bihar Agricultural University, Sabour, Bhagalpur-813 210, Bihar
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SWETA SINHA
Bihar Agricultural University, Sabour, Bhagalpur-813 210, Bihar
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S N SINGH
Bihar Agricultural University, Sabour, Bhagalpur-813 210, Bihar
Keywords:
Cluster distance, Genetic divergence, Multivariant analysis, Sesame, SSRAbstract
Sesame is one of the most important and ancient oilseeds crop with high and good quality oils content. Present Investigation was carried out for the assessment of the genetic diversity using 33 sesame genotypes through morphologically and 21 SSR markers. In the morphological diversity analysis, nine clusters were observed with 33 genotypes using all phenological, yield and its contributing attributes. Among the 9 clusters, cluster II had maximum number of genotypes (14) followed by cluster III (6), cluster IV (5), cluster I (3), cluster V, VI, VII, VIII and IX had one genotype to each cluster. The highest inter-cluster distance was observed between cluster VI and I (386.70), which indicates maximum diversity between the genotypes of these clusters, could be further used in crossing programme. However, SSR markers grouped 33 genotypes into six clusters at 0.51 dissimilarity index. In which cluster V had maximum (7) genotypes and cluster VI had minimum (3) genotypes. The results of genetic diversity tallied with the morphological diversity, such as BRT-04 are the farthest from BRT-08, BRT-09 and BRT-10, however BRT-08, BRT-09 and BRT-10 are in the same cluster in both morphological and molecular diversity analysis. The UPGMA dendrogramwas constructed using Jaccard's dissimilarity coefficients based on SSR markers score on thirty-three genotypes. Polymorphism information content (PIC) value varied between 0.15 and 0.36 with an average of 0.27. The genotypes from the distinct clusters can be utilized for hybridization programme to recover heterotic pools.
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References
Abate M and Mekib F 2015. Assessment of genetic variability and character association in Ethopian low-altitude sesame (SesamumindicumL.) genotypes. Journal of Advanced Studies in Agricultural, Biological and Environmental Science, 2(3): 55-66.
Badri J, Yepuri V, Ghanta A, Siva S and Siddiq E A 2014. Development of microsatellite markers in sesame (Sesamum indicum L.). Turkish Journal of Agriculture and Forestry, 38(5): 603-614.
Bamrotiya M M, Patel J B, Ribadiya K H and Chetariya C P 2016. Genetic divergence analysis in sesame (Sesamum indicum L.). AGRES-An International e-Journal, 5(3): 228-234.
Begum S, Islam M A, Husna A, Hafiz T B and Ratna M 2011. Genetic diversity analysis in sesame (S. indicum L.). SAARC Journal of Agriculture, 9(2): 65-71.
Bhat KV, Babrekar P P and Lakhanpaul S 1999. Study of genetic diversity in Indian and exotic sesame (Sesamum indicum L.) germplasmusing randomamplified polymorphic DNA(RAPD) markers. Euphytica, 110(1): 21-33.
Bhattacharjee M, Prakash S H, Roy S, Begum T and Dasgupta T 2018. DNA fingerprinting of CUMS 17 (Suprava): a newly developed variety of sesame. International Journal of Pure & Applied Bioscience, 6(5): 161-166.
Bhattacharyya U, Pandey SK, Dasgupta T 2014. Identification of EST-SSRs and FDM in sesame (Sesamum indicum L.) through data mining. Scholarly Journal of Agricultural Science, 4: 60-69.
Botstein D, White R L, Skolnick M and Davis R W 1980. Construction of a genetic linkage map in man using restriction fragment length polymorphisms. American Journal of Human Genetics, 32: 314-331.
Doyle J J and Doyle J L 1990. Isolation of plant DNA from fresh tissue. Focus, 12: 13-15. Ercan A G, Taskin M and Turgut K 2004. Analysis of genetic diversity in Turkish sesame (Sesamum indicum L.) populations using RAPD markers. Genetic Resources and Crop Evolution, 51(6): 599-607.
Gogoi L R, Singh S K and Sarma R N 2018. Assessment of genetic diversity in indigenous sesame genotypes. International Journal of Current Microbiology and Applied Sciences, 7(6): 1509-1520.
Iqbal A, Patil P K, Akhtar R, Begum T and Dasgupta T 2018. Diversity in sesame accessions. International Journal of Agriculture, Environment and Biotechnology, 11(5): 725-731.
Jadhav R S and Mohrir M N 2013. Genetic divergence analysis in sesame (Sesamum indicum L.). Electronic Journal of Plant Breeding, 4(1): 1090-1092.
Kim D H, Zur G, Danin-Poleg Y, Lee S W, Shim K B, Kang C W and Kashi Y 2002. Genetic relationships ofsesame germplasm collection as revealed by intersimple sequence repeats. Plant Breeding, 121(3): 259-262.
Kumar H, Kaur G and Banga S 2012. Molecular characterization and assessment of genetic diversity in sesame (Sesamum indicum L.) germplasmcollection using ISSR markers. Journal of Crop Improvement, 26(4): 540-557.
Kumhar S R and Rajani B 2021. Genetic analysis and diversity studies in sesame (Sesamum indicum L.). Journal of Oilseeds Research, 38(4): 329-336.
Kumhar S R and Solanki Z S 2009. Genetic diversity and variability in sesame (Sesamum indicum L.). Journal of Oilseeds Research, 26: 162-164.
Kumhar S R, ChoudharyB R and Paroha S 2013. Genetic diversity analysis for seed yield and quality characters in sesame (Sesamum indicum L.). Journal of Oilseeds Research, 30(2): 171-173.
Kumar V, Sinha S, Satyendra, Sinha S, Singh R S and Singh S N 2022. Assessment of genetic variability, correlation, path analysis in sesame (Sesamum indicum L.). Electronic Journal f Plant Breeding, 13(1): 208-215.
Laurentin H E and Karlovsky P 2006. Genetic relationship and diversity in a sesame (Sesamum indicum L.) germplasm collection using amplified fragment length polymorphism (AFLP). BMC Genetics, 7: 10.
Laurentin H and Karlovsky P 2007. AFLP fingerprinting ofsesame (Sesamum indicum L.) cultivars: identification, genetic relationship and comparison of AFLP informativeness parameters. Genetic Resources and Crop Evolution, 54(7):1437-1446.
Narayan R and Murugan S 2013. Studies on variability in sesame (Sesamum indicum L.). International Journal of Agricultural Research, 2(11): 52-55.
Pandey S K, Das A, Rai P and Dasgupta T 2015. Morphological and genetic diversity assessment of sesame Sesamum indicum L. accessions differing in origin. Physiology and Molecular Biology of Plants, 21: 519-529.
Parameshwarappa S G, Palakshappa M G and Shinde G G 2012. Studies on genetic divergence for yield and yield attributing traits in sesame (Sesamum indicum L.) germplasm. International Journal of Agricultural Sciences, 8(2): 441-444.
Rajitha D, Srikanth T, Padmaja D and Kiran Babu T 2021. Genetic variability and heritability studies among genotypes of sesame (Sesamum indicum L.). Journal of Oilseeds Research, 38(1): 43-48.
Rao C R 1952. Advanced statistical methods in biometrical research. John Wiley and Sons Inc., New York. pp. 390.
Rohlf F J 1998. NTSYS-pc Numerical Taxonomy and Multivariate Analysis System Version 2.0 User Guide. Applied Biostatistics Inc., Setauket, New York, pp. 37.
Salazar B, Laurentin H, Davila M, Castillo M A 2006. Reliability of the RAPD technique for germplasm analysis of sesame (Sesamum indicum L.) from Venezuela. Interciencia, 31(6): 456-460.
Singh A, Bisen R and Tiwari A 2018. Genetic variability and character association in sesame (Sesamum indicum L.) genotypes. International Journal of Current Microbiology Applied Sciences, 7(11): 2407-2415.
Soundharya B, Hemalatha V, Shobha rani T, Edukondalu B 2017. Genetic divergence studies in sesame (Sesamum indicum L.) genotypes. International Journal of Current Microbiology and Applied Sciences, 6(9): 2615-2619.
SpandanaB, PrathapRV, John PG, Anuradha G, Sivaramakrishnan S (2012) Development and characterization of microsatellite markers (SSR) in sesame (Sesamum indicum L.) species. Applied Biotechemistry Biotechnology, 168: 1594-1607.
Swathy V, Premnath A, Viswanathan P L, Raveendran M and Manonmani S. 2018. Determination of genetic divergence based onmorphological traits in sesame (Sesamum indicum L.). Electronic Journal of Plant Breeding, 9(2): 747-752.
Tripathi A, Bisen R, Ahirwal R P, Paroha S, Sahu R, Ranganatha A R G 2014. Study on genetic divergence in sesame (Sesamum indicum L.) germplasm based on morphological and quality traits. Bioscan, 8(4): 1387-1391.
Zhang Y X, Sun J A, Zhang X R, Wang L H and Che Z 2011. Analysis on genetic diversity and genetic basis of the main sesame cultivars released in China. Agricultural Sciences in China, 10(4): 509-518.
Zhang Y X, Zhang X R, Che Z, Wang L H, Wei W L and Li D H 2012. Genetic diversity assessment ofsesame core collection in China by phenotype and molecular markers and extraction of a mini-core collection. BMC Genetics, 13: 102.
