Morphological and molecular characterization of anamorph associated with gray mold of castor (Ricinus communis L.)
CHARACTERIZATION OF ANAMORPH ASSOCIATED WITH CASTOR GRAY MOLD
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Authors
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C YAMUNA
Prof. Jayashankar Telangana State Agricultural University, Rajendranagar, Hyderabad-500 030, Telangana State
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P KISHORE VARMA
Prof. Jayashankar Telangana State Agricultural University, Rajendranagar, Hyderabad-500 030, Telangana State
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R D PRASAD
Prof. Jayashankar Telangana State Agricultural University, Rajendranagar, Hyderabad-500 030, Telangana State
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K VIJAYA LAKSHMI
Prof. Jayashankar Telangana State Agricultural University, Rajendranagar, Hyderabad-500 030, Telangana State
Keywords:
Amphobotrys ricini, Botryotinia ricini, Castor gray mold, Molecular identificationAbstract
Graymold of castor caused by the anamorph ofBotryotinia ricini isthe most important disease and causessevere losses when there are heavy and continuous rains during capsule formation stage. In the present investigation, the anamorph of B. ricini was characterized based on morphological and molecular studies. The size of conidia and conidiophores were recorded from the culture grown on oat meal enriched medium for morphological characterization. Cultural characters like colony colour, growth pattern and sclerotial production were studied on six growth media.Based on microscopic observations, the anamorph wasidentified as Amphobotrysricini. Further, the identity was confirmed by amplifying fungal DNA using two universal Internal Transcribed Spacer (ITS) primers, ITS1 and ITS4 in a polymerase chain reaction. A single 0.5 kb band was amplified and amplified region is sequenced and analyzed in a standard Gen Bank BLAST search for fungal species identification. BLAST search has shown 99% similarity of the query sequence with Amphobotrys ricini. The results of this study confirmed that A. ricini is the anamorphic state associated with castor gray mold in Hyderabad region of Telangana state.
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References
Aswani Kumar G 2001. Management of gray mold disease in castor (Ricinus communis L.). M Sc Thesis, Acharya N G Ranga Agriculture University, Rajendranagar, Hyderabad.
Bheemaraju A 1999. Biology and management of grey mold of castor (Ricinus communis L.). M Sc Thesis, Acharya N G Ranga Agriculture University, Rajendranagar, Hyderabad.
Buchwald N F 1949. Studies in the Scletoriniaceae: I. Taxonomy of the Sclerotiniaceae. Kongelige Veterinær-og Landbohøjskole, Aarsskrift, 32: 1-116.
Hennebert G L 1973. Botrytis and Botrytis-like genera. Persoonia,7: 183-204.
Jarvis W R 1977. Botryotinia and Botrytis species: Taxonomy,Physiology and Pathogenicity, A guide to the Literature, Monograph No. 15, Canada Department of Agriculture,Ottawa, Canada.
Jin- Hyeuk Kwon, Mi-Geone Cheon, OkheeChoi and Jinwoo Kim2011. First report of Botrytis cinerea as postharvest pathogenof bluebertry in Korea. The Korean Society of Mycology, 39:52-53.
Khazaeli P, Zamanizadeh H, Morid and Bayat H 2010.Morphological and molecular identification of Botrytis cinerea, causal agent of gray mold in rose greenhouses in central regions of Iran. International Journal of Agricultural Science and Research, 1: 19-24.
Mirzaei S, Goltapeh E M and Shams-bakhsh M 2007. Taxonomical studies on the genus Botrytis in Iran. Journal of Agricultural Technology, 3: 65-76.
Moses G J and Reddy R R 1989. Grey rot of castor in AndhraPradesh. Journal of Research ANGRAU, 17: 74-75.
Nielsen K, Justesen A F, Jensen D F and Yohalem D S 2001.Universally primed polymerase chain reaction alleles and internal transcribed spacer restriction fragment lengthpolymorphisms distinguish two subgroups in Botrytis aclada distinct from B. byssoidea. Phytopathology, 91: 527-533.
Sambrook J, Fritsch E F and Maniatis T 1989. Molecular Cloning: a Laboratory Manual, 2nd edn.,Cold Spring Harbor LaboratoryPress, Cold Spring Harbor, New York.
Soares D J 2012. Gray mold of castor: A review. In: Plant Pathology. Cumagan C J R (ed), In Tech, Rijeka, pp. 219-240.
Sung-Kee H, Wan-Gyu K, Weon-Dae C and Hong-Gi K 2001. Occurrence of gray mold in castor bean caused by Botrytis cinerea and Amphobotrysricini in Korea. ThePlantPathology Journal, 17: 357-360.
White T J, Bruns T, Lee S and Taylor J 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: PCR Protocols: A Guide to Methods and Applications, Innis M A, Gelfand D H, Sninsky J J, White T J (eds), Academic Press, New York, USA, pp. 315-322.
