Callus induction studies in various explants in different genotypes of sesame (Sesamum indicum L.)
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CALLUS INDUCTION STUDIES IN VARIOUS EXPLANTS IN DIFFERENT GENOTYPES OF SESAME
Authors
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S RAGHU DEEPIKA
College of Agriculture, Acharya N.G. Ranga Agricultural University, Rajendranagar-500 030, Hyderabad
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N A ANSARI
College of Agriculture, Acharya N.G. Ranga Agricultural University, Rajendranagar-500 030, Hyderabad
Keywords:
Callus induction, Explants, Genotypes, Hormones, SesameAbstract
The goal of this study was to develop a suitable protocol for callus induction in sesame, Sesamum indicum which can be further used for regeneration studies. The experiments were carried out using three explants viz., cotyledonary leaf, hypocotyls and primary leaf at different hormonal combinations and concentrations to study callus induction. There was significant difference in the response of explants to different hormone treatments although there is no genotypic difference with regard to explants. Results showed that callus induction was significantly influenced by explants type. Hypocotyls responded well for callus induction in most of the genotypes. The callus induction frequency was significantly reduced (36.5%) when 0.2 mg BA was used along with 0.2 mg/l
NAA. Replacing this hormonal combination with 2.0mg/l BA and 0.2 mg/l NAA greatly improved the callus induction frequency (51.4%).
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References
Armstrong C L and Phillips R L 1988. Genetic and cytogenetic variation in plants regenerated from organogenic and friable, embryogenic tissue cultures of maize. Crop Science, 28: 363-369.
FAO 2012. Production Crops : Sesame Seeds. Food and Agriculture Organization of the United Nations.
Jeyamary R and Jayabalan N 1997. Influence of growth regulators on somatic embryogenesis in sesame. Plant Cell, Tissue and Organ Culture, 49: 67-70.
Lazzeri P A, Hildebrand D F and Collins G B 1987. Soyabean somatic embryogenesis: Effect of hormone and culture manipulation. Plant Cell, Tissue and Organ Culture, 10: 197-208.
Ram R, Catlin D, Romero J and Cowley C 1990. Sesame: New approaches for crop improvement. In: Janick J and Simon J E (Ed.) Advances in New Crops. Timber Press, Portland, pp. 225-228.
Rao K R and Vaidyanath K 1997. Induction of multiple shoots from seedling shoots tips of different varieties of sesamum. Indian Journal of Plant Physiology, 2: 257-261.
Sharma M and Pareek L K 1998. Direct shoot bud differentiation from different explants of in vitro regenerated shoots in sesame. Journal of Phytological Research, 11: 161-163.
Sudhakar D and Rangaswamy S R 1989. Sesame biotechnology: Embryo culture in Sesamum indicum L. Oil Crops Newsletter, 6: 48-50.
Xu Z Q, Jia J F and Hu Z D 1997. Somatic embryogenesis in Sesamum indicum L.cv. nigrum. Journal of Plant Physiology, 150(6): 755-758.
Younghee K 2001. Effects of BA, NAA, 2,4-D and AgNO3 treatments on the callus induction and shoot regeneration from hypocotyl and cotyledon of sesame (Sesamum indicum L.). Journal of the Korean Society for Horticultural Science, 42(1): 70-74.
